James Van Alstine
GE Healthcare
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Publication
Featured researches published by James Van Alstine.
Journal of Chromatography A | 2008
Kristian Becker; James Van Alstine; Leif Bülow
A multifunctional peptide tag (HYDHYD) consisting of histidine, tyrosine and aspartate residues was fused to the N-terminal ends of green fluorescent protein (GFP), lactate dehydrogenase (LDH) and human hemoglobin (Hb), proteins which were subjected to ion-exchange chromatography (IEC), aqueous two-phase system partition, immobilized metal-ion affinity chromatography (IMAC), and hydrophobic interaction chromatography (HIC). Tagged GFP was retained significantly longer (>1 column volume) in both HIC and IEC. It exhibited 3x greater partition in favor of the hydrophobic phase in a two-phase system and 96% could be bound to an IMAC column which did not bind native GFP.
Chemical Engineering Science | 2006
Conan J. Fee; James Van Alstine
Biotechnology and Bioengineering | 2007
Anders Ljunglöf; Karol Lacki; Jay Mueller; Chithkala Harinarayan; Robert van Reis; Robert L. Fahrner; James Van Alstine
Methods of biochemical analysis | 2011
Conan J. Fee; James Van Alstine
Archive | 2009
Mattias Algotsson; Hans Berg; Åsa Bjurling; Christian Kaisermayer; Bjorn Noren; Nicholas Thevenin; James Van Alstine
Archive | 2004
James Van Alstine; Jan Bergstrom; Henrik Ihre; Tobias Soderman
Archive | 2008
Hans O. Johansson; James Van Alstine; Rolf Hjorth; Karol Lacki; Emmanuel Macedo; Gunnar Malmquist; Jamil Shanagar
Archive | 2012
James Van Alstine; Mikael Berg; Johanna Kjörning; Jamil Shanagar
Archive | 2008
Rolf Hjorth; Karol Lacki; Emmanuel Macedo; Gunnar Malmquist; Jamil Shanagar; James Van Alstine
Archive | 2007
Gregory Daryll Goddard; Mohan Mark Amaratunga; Cyndy D. Sanberg; James Van Alstine; Oleg Kuzmenok
