Jan Buček

Zdeněk Lukáš; Pavel Dráber; Jan Buček; Eduarda Dráberová; Svatopluk Doležel

The expression of vimentin and the phosphorylated variant of high molecular weight neurofilament protein (NF-H) was studied in developing human fetal dorsal root ganglia and spinal cord. The technique used for examination of cryosections was double-label fluorescence with monoclonal antibodies. Both proteins were present in the nerve fibres inside the ganglia of 6- and 8-week-old embryos. During further development the expression of vimentin continued to increase in the satellite cells, but was found to be decreasing in the ganglion cells. Phosphorylated NF-H was found in the processes of ganglion cells, as well as in the perikarya at all developmental stages. In the spinal cord of 6- and 8-week-old embryos, phosphorylated NF-H protein was found in the longitudinal fibres of the marginal layer and in processes of the mantle zone; some of the fibres also contained vimentin. Later the co-expression of the two proteins ceased and vimentin was found only in glial and mesenchymal derivatives. Phosphorylated NF-H was located, at all developmental stages, in the axons of both white and grey matter, but not in the neuronal perikarya. The results indicate that phosphorylation of the NF-H in human dorsal root ganglia starts in the perikarya of the ganglion cells while in the ganglion cells of the spinal cord it takes place in the axons.

Miroslav Krejčí; Robert Staffa; Dvorák M; Radim Holub; Bohuslav Vojtíšek; Z. Kríž; Zdeněk Konečný; Jan Buček

BACKGROUND Infection represents a less frequent cause for the development of an abdominal aortic aneurysm. The diagnosis is associated with a high risk of rapir progression and rupture. CASE REPORTS Case 1 is a female operated on urgently for a ruptured mycotic aneurysm of the abdominal aorta. After resection of the aneurysm, we performed in situ replacement using a rifampicin-soaked vascular prosthesis. In case 2, an asymptomatic aneurysm of the right iliac artery was treated by endovascular implantation of a stent-graft. Both patients were treated with antibiotics concurrently and are still alive. DISCUSSION The discussion deals with the up-to-date treatment modalities for mycotic aneurysms in the aorto-iliac region. The indication criteria must be adjusted individually. CONCLUSION The prognosis of patients with a mycotic aneurysm depends particularly on an early diagnosis.

Robert Staffa; Kríz Z; Robert Vlachovský; Dvorák M; Tomáš Novotný; Jan Buček; Stanislav Šárník; Bohuslav Vojtíšek

Miroslav Krejčí; Robert Staffa; Zdeněk Kříž; Jan Buček

Robert Vlachovský; Robert Staffa; Tomáš Novotný; Jan Buček

Robert Vlachovský; Jan Buček; Robert Staffa

Robert Vlachovský; Robert Staffa; Jan Buček

Robert Staffa; Zdeněk Kříž; Jan Buček; Robert Vlachovský; Tomáš Novotný

Luboš Kubíček; Robert Staffa; Zdeněk Kříž; Jan Buček

Hana Pluháčková; Robert Staffa; Tomas Novotny; Kríz Z; Jan Buček