Jan K. Hongslo

Effect of sodium fluoride on LS cells.

The fluoride sensitivity and the lethal concentration of LS cells were determined. The cells were adapted to grow in the presence of lethal concentrations of fluoride. The adaptation persisted after removal of fluoride. Any possible binding of the fluoride to medium components was excluded.

Cellular resistance to fluoride.

Cells cultured in vitro can be adapted to grow in the presence of high concentrations of fluoride by stepwise increasing the fluoride concentration in the culture medium. Such cells seem to have the ability to exclude fluoride from intracellular compartments.

Glucose consumption and lactate production in normal and fluoride adapted LS cells

The established cell line LS (mouse fibroblasts) has been adapted to grow at otherwise toxic concentrations of fluoride. Different substrains of adapted LS cells consumed less glucose and converted less of the glucose to lactate than the unadapted cells. Differences were also found in the two parameters dependent on the number of generations grown in the presence of fluoride. The more generations with fluoride, the less lactate was produced and the less glucose consumed.