Jan Pawlowski

The revised classification of eukaryotes.

This revision of the classification of eukaryotes, which updates that of Adl et al. [J. Eukaryot. Microbiol. 52 (2005) 399], retains an emphasis on the protists and incorporates changes since 2005 that have resolved nodes and branches in phylogenetic trees. Whereas the previous revision was successful in re‐introducing name stability to the classification, this revision provides a classification for lineages that were then still unresolved. The supergroups have withstood phylogenetic hypothesis testing with some modifications, but despite some progress, problematic nodes at the base of the eukaryotic tree still remain to be statistically resolved. Looking forward, subsequent transformations to our understanding of the diversity of life will be from the discovery of novel lineages in previously under‐sampled areas and from environmental genomic information.

First insights into the biodiversity and biogeography of the Southern Ocean deep sea

Shallow marine benthic communities around Antarctica show high levels of endemism, gigantism, slow growth, longevity and late maturity, as well as adaptive radiations that have generated considerable biodiversity in some taxa. The deeper parts of the Southern Ocean exhibit some unique environmental features, including a very deep continental shelf and a weakly stratified water column, and are the source for much of the deep water in the world ocean. These features suggest that deep-sea faunas around the Antarctic may be related both to adjacent shelf communities and to those in other oceans. Unlike shallow-water Antarctic benthic communities, however, little is known about life in this vast deep-sea region. Here, we report new data from recent sampling expeditions in the deep Weddell Sea and adjacent areas (748–6,348 m water depth) that reveal high levels of new biodiversity; for example, 674 isopods species, of which 585 were new to science. Bathymetric and biogeographic trends varied between taxa. In groups such as the isopods and polychaetes, slope assemblages included species that have invaded from the shelf. In other taxa, the shelf and slope assemblages were more distinct. Abyssal faunas tended to have stronger links to other oceans, particularly the Atlantic, but mainly in taxa with good dispersal capabilities, such as the Foraminifera. The isopods, ostracods and nematodes, which are poor dispersers, include many species currently known only from the Southern Ocean. Our findings challenge suggestions that deep-sea diversity is depressed in the Southern Ocean and provide a basis for exploring the evolutionary significance of the varied biogeographic patterns observed in this remote environment.

Phylogenomics Reshuffles the Eukaryotic Supergroups

Background Resolving the phylogenetic relationships between eukaryotes is an ongoing challenge of evolutionary biology. In recent years, the accumulation of molecular data led to a new evolutionary understanding, in which all eukaryotic diversity has been classified into five or six supergroups. Yet, the composition of these large assemblages and their relationships remain controversial. Methodology/Principle Findings Here, we report the sequencing of expressed sequence tags (ESTs) for two species belonging to the supergroup Rhizaria and present the analysis of a unique dataset combining 29908 amino acid positions and an extensive taxa sampling made of 49 mainly unicellular species representative of all supergroups. Our results show a very robust relationship between Rhizaria and two main clades of the supergroup chromalveolates: stramenopiles and alveolates. We confirm the existence of consistent affinities between assemblages that were thought to belong to different supergroups of eukaryotes, thus not sharing a close evolutionary history. Conclusions This well supported phylogeny has important consequences for our understanding of the evolutionary history of eukaryotes. In particular, it questions a single red algal origin of the chlorophyll-c containing plastids among the chromalveolates. We propose the abbreviated name ‘SAR’ (Stramenopiles+Alveolates+Rhizaria) to accommodate this new super assemblage of eukaryotes, which comprises the largest diversity of unicellular eukaryotes.

The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing.

Current sampling of genomic sequence data from eukaryotes is relatively poor, biased, and inadequate to address important questions about their biology, evolution, and ecology; this Community Page describes a resource of 700 transcriptomes from marine microbial eukaryotes to help understand their role in the worlds oceans.

The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy

The interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack of taxonomically curated reference data sets for the targeted genes. The Protist Ribosomal Reference database (PR2, http://ssu-rrna.org/) provides a unique access to eukaryotic small sub-unit (SSU) ribosomal RNA and DNA sequences, with curated taxonomy. The database mainly consists of nuclear-encoded protistan sequences. However, metazoans, land plants, macrosporic fungi and eukaryotic organelles (mitochondrion, plastid and others) are also included because they are useful for the analysis of high-troughput sequencing data sets. Introns and putative chimeric sequences have been also carefully checked. Taxonomic assignation of sequences consists of eight unique taxonomic fields. In total, 136 866 sequences are nuclear encoded, 45 708 (36 501 mitochondrial and 9657 chloroplastic) are from organelles, the remaining being putative chimeric sequences. The website allows the users to download sequences from the entire and partial databases (including representative sequences after clustering at a given level of similarity). Different web tools also allow searches by sequence similarity. The presence of both rRNA and rDNA sequences, taking into account introns (crucial for eukaryotic sequences), a normalized eight terms ranked-taxonomy and updates of new GenBank releases were made possible by a long-term collaboration between experts in taxonomy and computer scientists.

CBOL Protist Working Group: Barcoding Eukaryotic Richness beyond the Animal, Plant, and Fungal Kingdoms

A group of protist experts proposes a two-step DNA barcoding approach, comprising a universal eukaryotic pre-barcode followed by group-specific barcodes, to unveil the hidden biodiversity of microbial eukaryotes.

Phylogenomics reveals a new ‘megagroup’ including most photosynthetic eukaryotes

Advances in molecular phylogeny of eukaryotes have suggested a tree composed of a small number of supergroups. Phylogenomics recently established the relationships between some of these large assemblages, yet the deepest nodes are still unresolved. Here, we investigate early evolution among the major eukaryotic supergroups using the broadest multigene dataset to date (65 species, 135 genes). Our analyses provide strong support for the clustering of plants, chromalveolates, rhizarians, haptophytes and cryptomonads, thus linking nearly all photosynthetic lineages and raising the question of a possible unique origin of plastids. At its deepest level, the tree of eukaryotes now receives strong support for two monophyletic megagroups comprising most of the eukaryotic diversity.

Invasive alga reaches California

The recent discovery of the marine green alga Caulerpa taxifolia on the Californian coast has raised public concern about the potential danger of a new invasion similar to the one endured by the Mediterranean Sea over the past decade. A small colony of C. taxifolia introduced into the Mediterranean in 1984 from a public aquarium has spread to more than 6,000 hectares today, outcompeting native species and seriously reducing diversity in areas of the northwestern Mediterranean. This invasive strain of C. taxifolia differs from tropical populations in that it is much larger, grows more vigorously, does not rely on sexual reproduction, and is resistant to low temperatures. Here we evaluate the risk of invasion by Californian C. taxifolia by comparing it genetically with the Mediterranean and aquarium strain, as well as with native tropical populations. Our results show that the Californian alga is the same as the invasive Mediterranean strain, calling for its rapid eradication to prevent a new invasion.

How many novel eukaryotic 'kingdoms'? Pitfalls and limitations of environmental DNA surveys

BackgroundOver the past few years, the use of molecular techniques to detect cultivation-independent, eukaryotic diversity has proven to be a powerful approach. Based on small-subunit ribosomal RNA (SSU rRNA) gene analyses, these studies have revealed the existence of an unexpected variety of new phylotypes. Some of them represent novel diversity in known eukaryotic groups, mainly stramenopiles and alveolates. Others do not seem to be related to any molecularly described lineage, and have been proposed to represent novel eukaryotic kingdoms. In order to review the evolutionary importance of this novel high-level eukaryotic diversity critically, and to test the potential technical and analytical pitfalls and limitations of eukaryotic environmental DNA surveys (EES), we analysed 484 environmental SSU rRNA gene sequences, including 81 new sequences from sediments of the small river, the Seymaz (Geneva, Switzerland).ResultsBased on a detailed screening of an exhaustive alignment of eukaryotic SSU rRNA gene sequences and the phylogenetic re-analysis of previously published environmental sequences using Bayesian methods, our results suggest that the number of novel higher-level taxa revealed by previously published EES was overestimated. Three main sources of errors are responsible for this situation: (1) the presence of undetected chimeric sequences; (2) the misplacement of several fast-evolving sequences; and (3) the incomplete sampling of described, but yet unsequenced eukaryotes. Additionally, EES give a biased view of the diversity present in a given biotope because of the difficult amplification of SSU rRNA genes in some taxonomic groups.ConclusionsEnvironmental DNA surveys undoubtedly contribute to reveal many novel eukaryotic lineages, but there is no clear evidence for a spectacular increase of the diversity at the kingdom level. After re-analysis of previously published data, we found only five candidate lineages of possible novel high-level eukaryotic taxa, two of which comprise several phylotypes that were found independently in different studies. To ascertain their taxonomic status, however, the organisms themselves have now to be identified.

Morphological distinction of molecular types in Ammonia – towards a taxonomic revision of the world’s most commonly misidentified foraminifera

Abstract In this study, morphometric analysis has been performed on 178 Ammonia specimens belonging to 12 different molecular types, plus non-sequenced type specimens of Ammonia beccarii and A. tepida. Molecular type distinction is based on phylogenetic analysis of 267 partial LSU rDNA sequences, obtained from 202 living Ammonia specimens, sampled in 30 localities from 17 countries bordering the Pacific Ocean, Atlantic Ocean, Mediterranean Sea, Caribbean Sea and North Sea. Restriction fragment length polymorphism (analysis was carried out for another seven specimens. Morphometric analysis was based on measurements or assessments of 37 external test characters in spiral, umbilical, profile and close-up Scanning Electron Microscopic views. Cluster analysis, canonical variates analysis, and detrended correspondence analysis, performed on the morphological data set, suggest that each molecular type can be distinguished morphologically and can be regarded as a separate species. Primary types of A. tepida and topotypes of A. beccarii are shown to be morphologically separate from any of the molecular types so far recognised. We are aware of at least 9 more distinctive morphotypes that have not yet been sequenced, and thus we infer that the total number of genetically distinct and morphologically separable living species of Ammonia worldwide is likely to exceed 25–30. At this stage not all molecular types can be unequivocally assigned to formally described species. Several genetically-based species can be distinguished by the presence of one distinct character, but most are discriminated on the basis of a combination of many different characters. Morphological characters (e.g. test shape, chamber shape, porosity, prolocular diameter, folium shape, radial furrow length, umbilical diameter) are shown to be slightly more valuable in separating the molecular types than surficial ornament (beads, pustules, bosses, secondary calcite). One highly distinctive group (2–3 species – beccarii, batava, ?inflata) is readily discriminated on the basis of its large test size, strongly beaded and grooved ornament, and the presence of fissures along the sutures on the spiral side. The results of this study imply that the widespread practice of recognising only one, two or three species of Recent Ammonia worldwide should be abandoned. The most commonly used name, Ammonia beccarii, should be restricted to a large, compressed, highly ornamented species, so far not recognised beyond its type locality in the Adriatic Sea. Other commonly used names, such as A. parkinsoniana and A. tepida, apply to species with far more restricted distributions than the literature would suggest.