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Dive into the research topics where Jana Klouckova is active.

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Featured researches published by Jana Klouckova.


Journal of Endocrinology | 2016

Endocrine effects of duodenal-jejunal exclusion in obese patients with type 2 diabetes mellitus.

Petra Kaválková; Miloš Mráz; Pavel Trachta; Jana Klouckova; Anna Cinkajzlova; Lacinová Z; Denisa Haluzikova; Marek Beneš; Zuzana Vlasáková; Václav Burda; Daniel Novák; T. Petr; Libor Vitek; Terezie Pelikánová; Martin Haluzik

Duodenal-jejunal bypass liner (DJBL) is an endoscopically implantable device designed to noninvasively mimic the effects of gastrointestinal bypass operations by excluding the duodenum and proximal jejunum from the contact with ingested food. The aim of our study was to assess the influence of DJBL on anthropometric parameters, glucose regulation, metabolic and hormonal profile in obese patients with type 2 diabetes mellitus (T2DM) and to characterize both the magnitude and the possible mechanisms of its effect. Thirty obese patients with poorly controlled T2DM underwent the implantation of DJBL and were assessed before and 1, 6 and 10months after the implantation, and 3months after the removal of DJBL. The implantation decreased body weight, and improved lipid levels and glucose regulation along with reduced glycemic variability. Serum concentrations of fibroblast growth factor 19 (FGF19) and bile acids markedly increased together with a tendency to restoration of postprandial peak of GLP1. White blood cell count slightly increased and red blood cell count decreased throughout the DJBL implantation period along with decreased ferritin, iron and vitamin B12 concentrations. Blood count returned to baseline values 3months after DJBL removal. Decreased body weight and improved glucose control persisted with only slight deterioration 3months after DJBL removal while the effect on lipids was lost. We conclude that the implantation of DJBL induced a sustained reduction in body weight and improvement in regulation of lipid and glucose. The increase in FGF19 and bile acids levels could be at least partially responsible for these effects.


Neurodegenerative Diseases | 2016

Mutated Huntingtin Causes Testicular Pathology in Transgenic Minipig Boars

Monika Macakova; Bozena Bohuslavova; Petra Vochozkova; Antonin Pavlok; Daniela Vidinska; Klara Vochyanova; Irena Liskova; Ivona Valekova; Monika Baxa; Zdenka Ellederova; Jiri Klima; Stefan Juhas; Jana Juhasova; Jana Klouckova; Martin Haluzik; Jiri Klempir; Hana Hansikova; Jana Spacilova; Ryan L. Collins; Ian Blumenthal; Michael E. Talkowski; James F. Gusella; David Howland; Marian DiFiglia; Jan Motlik

Background: Huntingtons disease is induced by CAG expansion in a single gene coding the huntingtin protein. The mutated huntingtin (mtHtt) primarily causes degeneration of neurons in the brain, but it also affects peripheral tissues, including testes. Objective: We studied sperm and testes of transgenic boars expressing the N-terminal region of human mtHtt. Methods: In this study, measures of reproductive parameters and electron microscopy (EM) images of spermatozoa and testes of transgenic (TgHD) and wild-type (WT) boars of F1 (24-48 months old) and F2 (12-36 months old) generations were compared. In addition, immunofluorescence, immunohistochemistry, Western blot, hormonal analysis and whole-genome sequencing were done in order to elucidate the effects of mtHtt. Results: Evidence for fertility failure of both TgHD generations was observed at the age of 13 months. Reproductive parameters declined and progressively worsened with age. EM revealed numerous pathological features in sperm tails and in testicular epithelium from 24- and 36-month-old TgHD boars. Moreover, immunohistochemistry confirmed significantly lower proliferation activity of spermatogonia in transgenic testes. mtHtt was highly expressed in spermatozoa and testes of TgHD boars and localized in all cells of seminiferous tubules. Levels of fertility-related hormones did not differ in TgHD and WT siblings. Genome analysis confirmed that insertion of the lentiviral construct did not interrupt any coding sequence in the pig genome. Conclusions: The sperm and testicular degeneration of TgHD boars is caused by gain-of-function of the highly expressed mtHtt.


Nutrition & Diabetes | 2018

Angiopoietin-like protein 3 and 4 in obesity, type 2 diabetes mellitus, and malnutrition: the effect of weight reduction and realimentation

Anna Cinkajzlova; Miloš Mráz; Lacinová Z; Jana Klouckova; Petra Kaválková; Helena Kratochvilova; Pavel Trachta; Jarmila Křížová; Denisa Haluzikova; Jan Škrha; Hana Papežová; Martin Haluzik

BackgroundAngiopoietin-like proteins (ANGPTLs) 3 and 4 are circulating factors that participate in the regulation of lipid and glucose metabolism.Subjects and methodsWe measured serum ANGPTL3 and 4 levels in 23 patients with obesity, 40 patients with obesity and type 2 diabetes mellitus (T2DM), 22 patients with anorexia nervosa (AN), 15 subjects undergoing 72-h fasting, and 12 patients with short bowel syndrome (SBS), and their changes after very-low-calorie diet (VLCD), bariatric surgery, partial realimentation, acute fasting, and parenteral nutrition in order to assess their possible role in metabolic regulations.ResultsSerum ANGPTL4 levels were higher in obese subjects without/with T2DM (94.50 ± 9.51 and 134.19 ± 7.69 vs. 50.34 ± 4.22 ng/ml, p < 0.001) and lower in subjects with AN relative to healthy control subjects (38.22 ± 4.48 vs. 65.80 ± 7.98 ng/ml, p = 0.002), while serum ANGPTL3 levels demonstrated inverse tendency. Nutritional status had no effect on ANGPTL3 and 4 mRNA expression in adipose tissue. Fasting decreased ANGPTL3 and increased ANGPTL4 levels, while VLCD reduced only ANGPTL3. Bariatric surgery and realimentation of AN or SBS patients had no effect on either ANGPTL. Multiple regression analysis identified BMI as an independent predictor of ANGPTL3; and BMI and HbA1c as independent predictors of ANGPTL4, respectively.ConclusionsTaken together, our data suggest that serum ANGPTL3 and 4 levels are influenced by nutritional status and fasting and could be involved in the metabolic disturbances present in obesity and AN.


Diabetes, Obesity and Metabolism | 2017

The effect of continuous exenatide infusion on cardiac function and perioperative glucose control in cardiac surgery patients: a single-blind, randomized, controlled trial

Michal Lips; Miloš Mráz; Jana Klouckova; Petr Kopecký; Milos Dobias; Jarmila Křížová; Jaroslav Lindner; Michaela Diamant; Martin Haluzik

We performed a randomized controlled trial with the glucagon‐like peptide‐1 (GLP‐1) receptor agonist exenatide as add‐on to standard peri‐operative insulin therapy in patients undergoing elective cardiac surgery. The aims of the study were to intensify peri‐operative glucose control while minimizing the risk of hypoglycaemia and to evaluate the suggested cardioprotective effects of GLP‐1‐based treatments. A total of 38 patients with decreased left ventricular systolic function (ejection fraction ≤50%) scheduled for elective coronary artery bypass grafting (CABG) were randomized to receive either exenatide or placebo in a continuous 72‐hour intravenous (i.v.) infusion on top of standard peri‐operative insulin therapy. While no significant difference in postoperative echocardiographic variables was found between the groups, participants receiving exenatide showed improved peri‐operative glucose control as compared with the placebo group (average glycaemia 6.4 ± 0.5 vs 7.3 ± 0.8 mmol/L; P < .001; percentage of time in target range of 4.5–6.5 mmol/L 54.8% ± 14.5% vs 38.6% ± 14.4%; P = .001; percentage of time above target range 39.7% ± 13.9% vs 52.8% ± 15.2%; P = .009) without an increased risk of hypoglycaemia (glycaemia <3.3 mmol/L: 0.10 ± 0.32 vs 0.21 ± 0.42 episodes per participant; P = .586). Continuous administration of i.v. exenatide in patients undergoing elective CABG could provide a safe option for intensifying the peri‐operative glucose management of such patients.


Endocrine Research | 2017

Angiopoietin-like protein 6 in patients with obesity, type 2 diabetes mellitus, and anorexia nervosa: The influence of very low-calorie diet, bariatric surgery, and partial realimentation.

Anna Cinkajzlova; Zdenka Lacinova; Jana Klouckova; Petra Kaválková; Pavel Trachta; Mikulas Kosak; Denisa Haluzikova; Hana Papezova; Miloš Mráz; Martin Haluzik

ABSTRACT Aim of the study: Angiopoietin-like protein 6 (ANGPTL6) is a circulating protein with a potential role in energy homeostasis. The aim of the study was to explore the changes in ANGPTL6 levels in patients with obesity (Body mass index, BMI > 40 kg/m2) with and without type 2 diabetes mellitus (T2DM) undergoing dietary intervention (very low calorie diet – VLCD) and in a subgroup of T2DM patients after bariatric surgery. Additionally, we examined changes in ANGPTL6 in anorexia nervosa (AN) patients at baseline and after partial realimentation. We also explored the changes in ANGPTL6 mRNA expression in subcutaneous adipose tissue (SAT) of obese subjects. Materials and methods: The study included 23 non-diabetic obese patients, 40 obese patients with T2DM (27 underwent VLCD and 13 underwent bariatric surgery), 22 patients with AN, and 37 healthy control subjects. Results: ANGPTL6 levels of AN patients were increased relative to the control group (68.6 ± 9.9 ng/ml) and decreased from 110.2 ± 13.3 to 73.6 ± 7.1 ng/ml (p = 0.004) after partial realimentation. Baseline ANGPTL6 levels in patients with obesity and T2DM did not differ from the control group. VLCD decreased ANGPTL6 levels only in obese patients with T2DM. Bariatric surgery induced a transient elevation of ANGPTL6 levels with a subsequent decrease to baseline levels. ANGPTL6 mRNA expression transiently increased after bariatric surgery and returned to baseline levels after 12 months. Conclusions: Collectively, our data suggest that serum ANGPTL6 levels and ANGPTL6 mRNA expression in SAT are affected by metabolic disorders and their treatment but do not appear to directly reflect nutritional status.


Blood | 2016

Calreticulin exposure by malignant blasts correlates with robust anticancer immunity and improved clinical outcome in AML patients

Jitka Fucikova; Iva Truxova; Michal Hensler; Etienne Becht; Lenka Kasikova; Irena Moserova; Sarka Vosahlikova; Jana Klouckova; Sarah E. Church; Isabelle Cremer; Oliver Kepp; Guido Kroemer; Lorenzo Galluzzi; Cyril Šálek; Radek Spisek


Cancer Immunology, Immunotherapy | 2018

Phase I/II trial of dendritic cell-based active cellular immunotherapy with DCVAC/PCa in patients with rising PSA after primary prostatectomy or salvage radiotherapy for the treatment of prostate cancer

Jitka Fucikova; Michal Podrazil; L. Jarolim; Pavla Bilkova; Michal Hensler; Etienne Becht; Zdenka Gašová; Jana Klouckova; Jana Kayserova; Rudolf Horvath; Anna Fialová; Katerina Vavrova; Klara Sochorova; Daniela Rozkova; Radek Spisek; Jirina Bartunkova


Physiological Research | 2016

Plasma concentrations and subcutaneous adipose tissue mRNA expression of clusterin in obesity and type 2 diabetes mellitus: the effect of short-term hyperinsulinemia, very-low-calorie diet and bariatric surgery.

Jana Klouckova; Lacinová Z; Petra Kaválková; Pavel Trachta; Mojmír Kasalický; Denisa Haluzikova; Miloš Mráz; Martin Haluzik


Diabetes | 2018

The Influence of Metabolic Surgery and Endoscopy on Serum Proteome in Subjects with Obesity and Type 2 Diabetes Mellitus

Miloš Mráz; Karel Harant; Helena Kratochvilova; Anna Cinkajzlova; Jana Klouckova; Petra Kaválková; Zdena Lacinova; Marek Beneš; Zuzana Vlasáková; Karin Dolezalova; Martin Fried; Terezie Pelikanova; Martin Haluzik


Diabetes | 2018

Subclinical Inflammation and Lymphocytes in Subcutaneous and Visceral Adipose Tissue—The Influence of Pregnancy and Gestational Diabetes Mellitus

Anna Cinkajzlova; Kateřina Anderlová; Zdena Lacinova; Jana Klouckova; Helena Kratochvilova; Hana Krejčí; Miloš Mráz; Martin Haluzik; Michal Krsek

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Martin Haluzik

Charles University in Prague

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Miloš Mráz

Charles University in Prague

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Anna Cinkajzlova

Charles University in Prague

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Petra Kaválková

Charles University in Prague

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Helena Kratochvilova

Charles University in Prague

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Pavel Trachta

Charles University in Prague

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Denisa Haluzikova

Charles University in Prague

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Zdena Lacinova

Charles University in Prague

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Lacinová Z

Charles University in Prague

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Michal Krsek

Charles University in Prague

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