Jana Liskova
Academy of Sciences of the Czech Republic
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Jana Liskova.
International Journal of Nanomedicine | 2015
Jana Liskova; Oleg Babchenko; Marian Varga; Alexander Kromka; Daniel Hadraba; Zdenek Svindrych; Zuzana Burdikova; Lucie Bacakova
Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings.
Biomedical Materials | 2014
Timothy Douglas; Wojciech Piwowarczyk; Elzbieta Pamula; Jana Liskova; David Schaubroeck; Sander C. G. Leeuwenburgh; Gilles Brackman; Lieve Balcaen; Rainer Detsch; Heidi Declercq; Katarzyna Cholewa-Kowalska; Agnieszka Dokupil; Vincent M.J.I. Cuijpers; Frank Vanhaecke; Ria Cornelissen; Tom Coenye; Aldo R. Boccaccini; Peter Dubruel
Hydrogels of biocompatible calcium-crosslinkable polysaccharide gellan gum (GG) were enriched with bioglass particles to enhance (i) mineralization with calcium phosphate (CaP); (ii) antibacterial properties and (iii) growth of bone-forming cells for future bone regeneration applications. Three bioglasses were compared, namely one calcium-rich and one calcium-poor preparation both produced by a sol-gel technique (hereafter referred to as A2 and S2, respectively) and one preparation of composition close to that of the commonly used 45S5 type (hereafter referred to as NBG). Incubation in SBF for 7 d, 14 d and 21 d caused apatite formation in bioglass-containing but not in bioglass-free samples, as confirmed by FTIR, XRD, SEM, ICP-OES, and measurements of dry mass, i.e. mass attributable to polymer and mineral and not water. Mechanical testing revealed an increase in compressive modulus in samples containing S2 and NBG but not A2. Antibacterial testing using biofilm-forming meticillin-resistant staphylococcus aureus (MRSA) showed markedly higher antibacterial activity of samples containing A2 and S2 than samples containing NBG and bioglass-free samples. Cell biological characterization using rat mesenchymal stem cells (rMSCs) revealed a stimulatory effect of NBG on rMSC differentiation. The addition of bioglass thus promotes GG mineralizability and, depending on bioglass type, antibacterial properties and rMSC differentiation.
Carbohydrate Polymers | 2015
Jana Liskova; Timothy Douglas; Jana Beranová; Agata Skwarczyńska; Mojca Božič; Sangram Keshari Samal; Zofia Modrzejewska; Selestina Gorgieva; Vanja Kokol; Lucie Bacakova
Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na-β-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1h, 3h and 6h incubation and slowed E. coli growth in liquid culture for 150min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth.
Journal of Functional Biomaterials | 2015
Jana Liskova; Lucie Bacakova; Agata Skwarczyńska; Olga Musial; Vitaliy Bliznuk; Karel A.C. De Schamphelaere; Zofia Modrzejewska; Timothy Douglas
Thermosensitive injectable hydrogels based on chitosan neutralized with sodium beta-glycerophosphate (Na-β-GP) have been studied as biomaterials for drug delivery and tissue regeneration. Magnesium (Mg) has been reported to stimulate adhesion and proliferation of bone forming cells. With the aim of improving the suitability of the aforementioned chitosan hydrogels as materials for bone regeneration, Mg was incorporated by partial substitution of Na-β-GP with magnesium glycerophosphate (Mg-GP). Chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were also loaded with the enzyme alkaline phosphatase (ALP) which induces hydrogel mineralization. Hydrogels were characterized physicochemically with respect to mineralizability and gelation kinetics, and biologically with respect to cytocompatibility and cell adhesion. Substitution of Na-β-GP with Mg-GP did not negatively influence mineralizability. Cell biological testing showed that both chitosan/Na-β-GP and chitosan/Na-β-GP/Mg-GP hydrogels were cytocompatible towards MG63 osteoblast-like cells. Hence, chitosan/Na-β-GP/Mg-GP hydrogels can be used as an alternative to chitosan/Na-β-GP hydrogels for bone regeneration applications. However the incorporation of Mg in the hydrogels during hydrogel formation did not bring any appreciable physicochemical or biological benefit.
Archive | 2016
Lucie Bacakova; Antonin Broz; Jana Liskova; Stepan Potocky Lubica Stankova; A. Kromka
Diamond in the allotrope form consists of carbon atoms arranged in a cubic crystal structure covalently bonded in sp3 hybridization. Diamond has emerged as a very promising material for various biomedical applications due to its excellent mechani‐ cal properties (hardness, low friction coefficient, good adhesiveness to the underlying substrate, good interlayer cohesion), optical properties (the ability to emit intrinsic luminescence), electrical properties (good insulator in the pristine state and semicon‐ ductor after doping), chemical resistance (low chemical reactivity and resistance to wet etching) and biocompatibility (little if any toxicity and immunogenicity). For ad‐ vanced biomedical applications, diamond is promising particularly in its nanostruc‐ tured forms, namely nanoparticles, nanostructured diamond films and composite scaffolds in which diamond nanoparticles are dispersed in a matrix (mainly nanodia‐ mond-loaded nanofibrous scaffolds). This chapter summarizes both our long-term experience and that of other research groups in studies focusing on the interaction of cells (particularly bone-derived cells) with nanodiamonds as nanoparticles, thin films and composites with synthetic polymers. Their potential applications in bioimaging, biosensing, drug delivery, biomaterial coating and tissue engineering are also reviewed.
Nanobiomaterials in Hard Tissue Engineering#R##N#Applications of Nanobiomaterials Volume 4 | 2016
Lucie Bacakova; Elena Filova; Jana Liskova; Ivana Kopova; Marta Vandrovcová; Jana Havlikova
Abstract Nanostructured materials, that is, planar materials with nanoscale surface roughness or three-dimensional nanostructured scaffolds (e.g., nanofibrous scaffolds or scaffolds with pores decorated with nanoparticles) are considered as excellent substrates for tissue engineering, because they mimic the nanoarchitecture of the natural extracellular matrix. On nanostructured substrates, the cell adhesion-mediating molecules, such as fibronectin, vitronectin, or collagen, are adsorbed in an appropriate geometrical conformation, which allows good accessibility of specific sites in these molecules (e.g., RGD-containing oligopeptides) for cell adhesion receptors. In addition, the nanostructured materials preferentially adsorb vitronectin, which is recognized with osteoblasts through the KRSR amino acid sequence. Thus, nanostructured substrates are particularly important for bone tissue engineering. On planar substrates, the surface nanoroughness can be created by various subtractive technologies, such as chemical etching, plasma treatment, sandblasting, shot peening, grinding, polishing, or additive technologies, such as deposition of various nanostructured films, based on ceramics, carbon nanoparticles, polymers, or metals. For construction of nanostructured 3D substrates, the nanoparticles can be admixed into the polymeric matrix or deposited on the inner structure of 3D scaffolds. These nanoparticles then reinforce the scaffolds and create additional nanostructure on the pore walls or fibers within the scaffolds, making these scaffolds stronger, more bioactive, and thus more suitable for bone tissue engineering. In our earlier studies and studies by other authors, all mentioned planar and 3D substrates proved to be suitable cell carriers for bone tissue engineering and construction of bone implants.
Journal of Tissue Engineering and Regenerative Medicine | 2018
Timothy Douglas; Michal Dziadek; Svetlana Gorodzha; Jana Liskova; Gilles Brackman; Valérie Vanhoorne; Chris Vervaet; Lieve Balcaen; María del Rosario Flórez García; Aldo R. Boccaccini; Venera Weinhardt; Tilo Baumbach; Frank Vanhaecke; Tom Coenye; Lucie Bacakova; Maria A. Surmeneva; Roman A. Surmenev; Katarzyna Cholewa-Kowalska; Andre G. Skirtach
Mineralization of hydrogel biomaterials is desirable to improve their suitability as materials for bone regeneration. In this study, gellan gum (GG) hydrogels were formed by simple mixing of GG solution with bioactive glass microparticles of 45S5 composition, leading to hydrogel formation by ion release from the amorphous bioactive glass microparticles. This resulted in novel injectable, self‐gelling composites of GG hydrogels containing 20% bioactive glass. Gelation occurred within 20 min. Composites containing the standard 45S5 bioactive glass preparation were markedly less stiff. X‐ray microcomputed tomography proved to be a highly sensitive technique capable of detecting microparticles of diameter approximately 8 μm, that is, individual microparticles, and accurately visualizing the size distribution of bioactive glass microparticles and their aggregates, and their distribution in GG hydrogels. The widely used melt‐derived 45S5 preparation served as a standard and was compared with a calcium‐rich, sol–gel derived preparation (A2), as well as A2 enriched with zinc (A2Zn5) and strontium (A2Sr5). A2, A2Zn, and A2Sr bioactive glass particles were more homogeneously dispersed in GG hydrogels than 45S5. Composites containing all four bioactive glass preparations exhibited antibacterial activity against methicillin‐resistant Staphylococcus aureus. Composites containing A2Zn5 and A2Sr5 bioactive glasses supported the adhesion and growth of osteoblast‐like cells and were considerably more cytocompatible than 45S5. All composites underwent mineralization with calcium‐deficient hydroxyapatite upon incubation in simulated body fluid. The extent of mineralization appeared to be greatest for composites containing A2Zn5 and 45S5. The results underline the importance of the choice of bioactive glass when preparing injectable, self‐gelling composites.
Microscopy Research and Technique | 2017
Jana Liskova; Daniel Hadraba; Elena Filova; Miroslav Konarik; Jan Pirk; Karel Jelen; Lucie Bacakova
Collagen often acts as an extracellular and intracellular marker for in vitro experiments, and its quality defines tissue constructs. To validate collagen detection techniques, cardiac valve interstitial cells were isolated from pigs and cultured under two different conditions; with and without ascorbic acid. The culture with ascorbic acid reached higher cell growth and collagen deposition, although the expression levels of collagen gene stayed similar to the culture without ascorbic acid. The fluorescent microscopy was positive for collagen fibers in both the cultures. Visualization of only extracellular collagen returned a higher correlation coefficient when comparing the immunolabeling and second harmonic generation microscopy images in the culture with ascorbic acid. Lastly, it was proved that the hydroxyproline strongly contributes to the second‐order susceptibility tensor of collagen molecules, and therefore the second harmonic generation signal is impaired in the culture without ascorbic acid.
Physica Status Solidi (a) | 2014
Lucie Bacakova; Ivana Kopova; Lubica Stankova; Jana Liskova; J. Vacik; V. Lavrentiev; Alexander Kromka; Stepan Potocky; Denisa Stranska
Materials Letters | 2018
Jana Liskova; Timothy Douglas; Robbe Wijnants; Sangram Keshari Samal; Ana Carina Loureiro Mendes; Ioannis S. Chronakis; Lucie Bacakova; Andre G. Skirtach