Jang Ho Joo

Library approach for reliable synthesis and properties of DNA-gold nanorod conjugates.

We developed a library-based approach to chemically stabilize cetyltrimethylammonium bromide (CTAB)-coated gold nanorods for the synthesis of polyvalent DNA-gold nanorod conjugates (DNA-AuNRs). Eleven chemical reagents were carefully chosen to constitute an additive library and screened by UV-vis spectroscopy to evaluate their stabilizing capability for the CTAB-coated AuNRs. Interestingly, 5-bromosalicylic acid (5-BrSA) was determined to most significantly stabilize the AuNRs by inducing additional adsorption of CTAB on the rod. Importantly, these stabilized AuNRs with 5-BrSA were conjugated with thiol DNA in an exceptionally reproducible and reliable method, which led to the systematic investigation of their cooperative assembly and disassembly properties under various conditions, including different types and lengths of the DNA sequences.

Recent advances in chemical functionalization of nanoparticles with biomolecules for analytical applications

The recent synthetic development of a variety of nanoparticles has led to their widespread application in diagnostics and therapeutics. In particular, the controlled size and shape of nanoparticles precisely determine their unique chemical and physical properties, which is highly attractive for accurate analysis of given systems. In addition to efforts toward controlling the synthesis and properties of nanoparticles, the surface functionalization of nanoparticles with biomolecules has been intensively investigated since the mid-1990s. The complicated yet programmable properties of biomolecules have proved to substantially enhance and enrich the novel functions of nanoparticles to achieve “smart” nanoparticle materials. In this review, the advances in chemical functionalization of four types of representative nanoparticle with DNA and protein molecules in the past five years are critically reviewed, and their future trends are predicted.

In vivo photothermal treatment by the peritumoral injection of macrophages loaded with gold nanoshells.

Photothermal treatment methods have been widely studied for their target specificity and potential for supplementing the limitations of conventional surgical treatments. In this study, we conducted in vivo photothermal treatments using macrophages containing nanoshells as live vectors. We injected macrophages at the peritumoral sites and observed that they had penetrated into the tumor approximately 48 hours after injection. Afterwards, we irradiated with a near-infrared laser for 2 minutes at 1 W/cm(2), causing cancer cell death. Our study identified the optimal conditions of the photothermal treatment and confirmed the feasibility of its use in in vivo treatments.

Synthesis of Gold Nanoparticle-Embedded Silver Cubic Mesh Nanostructures Using AgCl Nanocubes for Plasmonic Photocatalysis

A novel room-temperature aqueous synthesis for gold nanoparticle-embedded silver cubic mesh nanostructures using AgCl templates via a template-assisted coreduction method is developed. The cubic AgCl templates are coreduced in the presence of AuCl4- and Ag+ , resulting in the reduction of AuCl4- into gold nanoparticles on the outer region of AgCl templates, followed by the reduction of AgCl and Ag+ into silver cubic mesh nanostructures. Removal of the template clearly demonstrates the delicately designed silver mesh nanostructures embedded with gold nanoparticles. The synthetic mechanism, structural properties, and surface functionalization are spectroscopically investigated. The plasmonic photocatalysis of the cubic mesh nanostructures for the degradation of organic pollutants and removal of highly toxic metal ions is investigated; the photocatalytic activity of the cubic mesh nanostructures is superior to those of conventional TiO2 catalysts and they are catalytically functional even in natural water, owing to their high surface area and excellent chemical stability. The synthetic development presented in this study can be exploited for the highly elaborate, yet, facile design of nanomaterials with outstanding properties.

Deep-tissue imaging with collective accumulation of single scattering microscopy

We present an approach that maintains full optical resolution in imaging deep within scattering media. Imaging depth of 11.5 times the scattering mean free path was achieved with near-diffraction-limit resolution of 1.5 μm.