Janice L. Nerger

The relative numbers of long-wavelength-sensitive to middle-wavelength-sensitive cones in the human fovea centralis

The determination of the relative numbers of different cone types in the human retina is fundamental to our understanding of visual sensitivity and color vision; yet direct measurement which provide this basic information have not previously been made for all cone types. Here we present a model which links the detection of a test light of small dimension to the number of cones contributing to detection of the light. We selectively isolated either the long-wavelength-sensitive (L) or the middle-wavelength-sensitive (M) cones, by choosing combinations of wavelengths of adapting backgrounds and tests to favor detection by the cone class of interest. Our model was applied to the detection functions measured for six color normal observers to obtain estimates of the relative numbers of L to M cones. Our estimates ranged between 1.46 and 2.36 for our observers with a mean value near two L cones for every M cone in human fovea centralis.

The ratio of L cones to M cones in the human parafoveal retina

We present psychophysically-based estimates of the relative numbers of long-wavelength-sensitive (L) and middle-wavelength-sensitive (M) cones in the parafovea of three color-normal trichromats. Using methods previously applied to the fovea centralis, we obtained estimates of the relative numbers of L and M cones at retinal eccentricities of 1.0, 1.5, 2.0, 3.0, and 4.0 deg along the horizontal meridian of the temporal retina. Results for three observers indicate that the L to M cone ratio remains approximately invariant from the fovea to 4.0 deg eccentricity, with a mean ratio near 2:1.

Neural generators of ERPs linked with Necker cube reversals

Multistable perception occurs when a single physical stimulus leads to two or more distinct percepts that spontaneously switch (reverse). Previous ERP studies have reported reversal negativities and late positive components associated with perceptual reversals. The goal of the current study was to localize the neural generators of the reversal ERP components in order to evaluate their correspondence with previous fMRI results and to better understand their functional significance. A Necker-type stimulus was presented for brief intervals while subjects indicated their perceptions. Local auto-regressive average source analyses and dipole modeling indicated that sources for the reversal negativity were located in inferior occipital-temporal cortex. Generators of the late positive component were estimated to reside in inferior temporal and superior parietal regions.

The density of cones in the fovea centralis of the human dichromat.

We present estimates, based on psychophysical measurements, of the density of cones in the fovea centralis of human dichromats. The estimates for a group of three protanopes and three deuteranopes (this study) were compared to the estimates of the density of cones in a group of six color normal trichromats from previous studies (Cicerone & Nerger, 1985, 1989). The results support the conclusion that the density of cones in the fovea centralis of the dichromat is comparable to that of the color normal trichomat. These results tend not to support a model of dichromacy in which a class of cones as well as the associated pigment are lost in the dichromatic eye. Instead, dichromacy appears to involve a loss of one of the three visual pigments associated with human trichromacy, with a retention of the full numbers of cones.

The bimodality of unique green revisited

Unique green measurements were obtained from 50 females and 50 males under eight different experimental conditions. Combinations of two different test sizes (0.25 and 1.0 deg) and four different background fields (none, 62.5, 250, and 1000 td) comprised the experimental conditions under which unique green measurements were made. Group and gender frequency distributions of the unique green loci were examined for the eight experimental conditions. Differences in the shape of the frequency distributions were noted for the different test sizes and backgrounds as well as for gender, but none of the experimental parameters appeared to elicit a statistically significant bimodal distribution.

EFFECT OF THE S-CONE MOSAIC AND RODS ON RED/GREEN EQUILIBRIA

The loci of unique blue and unique yellow were measured with and without a rod bleach for various test sizes in the fovea and at 1 and 8 deg nasal and superior retinal eccentricities. Test sizes and retinal positions were selected to systematically manipulate the absolute and relative numbers of S cones underlying the test stimuli. The results revealed the following: (1) The locus of unique blue shifted to longer wavelengths as the absolute number of S cones underlying the test stimulus increased, suggesting that the S-cone neural weighting factor of the red/green (R/G) opponent model is linked to the absolute number of S cones. (2) In general, the locus of unique yellow remained invariant, although changes were observed in the superior retina. This finding indicates that either the L-to-M-cone ratio may not be invariant across all retinal quadrants or that this ratio may not determine the locus of unique yellow. (3) Rod signals affected the locus of the unique hues, especially at small test sizes, demonstrating an influence of rods on the R/G opponent mechanism.

Equilibrium hue judgements of dichromats

It is generally held that protanopes and deuteranopes see only regions of blues and yellows in the visible spectrum, with an achromatic point, called the neutral point, separating these regions. Considerations of a zone model of color vision for the dichromatic observer led us to predict that a reduced form of red/green discrimination would allow equilibrium blue judgements to be made by protanopes. We show that protanopes can make equilibrium blue determinations with as much reliability as they make neutral point settings. Our results indicate that protanopes but not deuteranopes are able to rely on a reduced form of red/green discrimination in the short wavelength part of the spectrum. Protanopes describe wavelengths longer than the neutral point as yellow. Between the neutral point and equilibrium blue, different wavelengths are described as having varying aspects of blue and green; and short of equilibrium blue they appear reddish blue. For dueteranopes, the spectrum longer than the neutral point appears yellow, and short of it, blue. The results of our experiments showing that the protanopic equilibrium blue is invariant with intensity variations, as it is in the trichromat, add support to the idea of a reduced form of red/green discrimination for protanopes. Our results also allow the evaluation of various models of protanopia.

Chromatic perceptive field sizes change with retinal illuminance

The effect of retinal illuminance (0.3-3.3 log td) on chromatic perceptive field size was investigated at 10 degrees eccentricity along the horizontal meridian of the temporal retina. Using the 4+1 color-naming procedure, observers described the hue and saturation of a series of monochromatic stimuli (440-660 nm, in 10-nm steps) of various test sizes (.098-5 degrees) after 30-min dark adaptation. Perceptive field sizes of the four elemental hues and the saturation component were estimated for each wavelength at each retinal illuminance. Results indicate that perceptive field sizes for blue, green, yellow, and saturation all decrease with increasing retinal illuminance; the perceptive field size for red is the smallest and invariant with intensity. The influence of rods on perceptive field size may account for some of the results; other factors are also considered.

A new look at the Bezold–Brücke hue shift in the peripheral retina

Experiments were conducted with a bipartite field to better understand the Bezold-Brücke hue shift in the peripheral retina. The first experiment measured hue shift in the fovea and at 1 degrees and 8 degrees along the horizontal meridian of the nasal retina for nominal test wavelengths of 430, 450, 490, 520 and 610 nm. Peripheral measurements were obtained under two adaptation conditions: after 30 min dark adaptation and following a rod-bleach. Results indicated that foveal hue shifts differed from those obtained after a rod-bleach. Data from the rod-bleach and no-bleach conditions in the periphery were similar, indicating that rods could not account for the differences between the foveal data and the rod-bleach peripheral data. Hue shifts obtained for the 520 nm test stimulus, and to a smaller extent other test wavelengths, at 8 degrees nasal retinal eccentricity revealed that the wavelength of the matching stimulus depended upon the lateral position of the matching and test fields, and this effect was greater in the no-bleach condition than the rod-bleach condition. Several factors were investigated in experiments 2 and 3 to explain the results with the 520 nm test field. It appears that differential rod density under the two half fields and the compression of photoreceptors by the optic disk may partially, but not fully, account for the 520 nm effect.

Color appearance of filled-in backgrounds affects hue cancellation, but not detection thresholds

A long-wavelength background can affect the appearance of an increment of light superimposed upon it in two ways. It can change the visual systems sensitivity to the increment, and it can change the appearance of the increment by directly adding redness to it. Through selective retinal-image stabilization, we evoked the filling-in phenomenon to change the appearance of 640- and 575-nm backgrounds. Either of these backgrounds could be made to appear red or yellow, depending upon whether it was viewed under stabilized or unstabilized conditions. When the appearance of the 640-nm background was altered by filling-in to appear less red, test probes superimposed upon it required less 540-nm component to achieve an equilibrium hue. Increment thresholds measured on the 640- and 575-nm backgrounds, however, did not change with the appearance of the backgrounds.