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Dive into the research topics where Janina Krywienczyk is active.

Publication


Featured researches published by Janina Krywienczyk.


Journal of Invertebrate Pathology | 1978

Occurrence of two serologically distinct groups within Bacillus thuringiensis serotype 3 ab var. kurstaki.

Janina Krywienczyk; Howard T. Dulmage; Paul G. Fast

Abstract Two groups distinguishable on the basis of crystal serology have been identified within Bacillus thuringiensis var kurstaki (serotype 3 ab). The toxicities of these two groups to Trichoplusia ni and Heliothis virescens expressed as T H ratio also differed. It is suggested that serotype 3 ab be separated into two subgroups designated as K-1 and K-73.


Journal of Invertebrate Pathology | 1969

Serological investigations of insect viruses. I. Comparison of three highly purified cytoplasmic-polyhedrosis viruses.

Janina Krywienczyk; Yugo Hayashi; F.T. Bird

Abstract Cytoplasmic-polyhedrosis viruses from Bombyx mori, Malacosoma disstria , and Orgyia leucostigma were isolated and purified by zonal sucrose gradient centrifugation. They were tested in double diffusion and immunoelectrophoresis on cellulose acetate membranes. It was found that the viruses from M. disstria and O. leucostigma are very closely related, and share at least five antigens one of which, at least, is shared with the virus from B. mori .


Journal of Invertebrate Pathology | 1974

Serological relationship between three varieties of Bacillus popilliae

Janina Krywienczyk; Peter Lüthy

Abstract The serological relationship between three varieties of Bacillus popilliae was investigated by double diffusion and immunoelectrophoresis, using antigens prepared from homogenized vegetative cells in the logarithmic growth phase. The results showed the existence of a close serological relationship among the three varieties in agreement with a recently proposed taxonomic scheme for the milky disease bacteria ( Wyss, 1971 ).


Journal of Invertebrate Pathology | 1966

Characteristics of a new strain of Bacillus thuringiensis var. thuringiensis berliner (serotype I) isolated from the bumblebee wax moth

G.E. Bucher; T. A. Angus; Janina Krywienczyk

Abstract A new strain of Bacillus thuringiensis, isolated from the bumblebee wax moth, Vitula edmandsae, has most of the biochemical and serological characteristics of strains designated as serotype I or as B. thuringiensis var. thuringiensis Berliner. However, the new strain is from 30 to 70 times as virulent for cutworm and silkworm larvae as previously isolated strains of serotype I, produces rapid paralysis in silkworms, and inhibits feeding of cutworm larvae. These effects indicate that it possesses some toxic components uncommon to serotype I strains. This conclusion is supported by the agardiffusion patterns obtained when antigen of the new strain reacts with antiserum prepared from parasporal crystals of other strains.


Journal of Invertebrate Pathology | 1970

Serological comparison of ribosomal and viral components

Janina Krywienczyk; Yugo Hayashi

Abstract Subcellular microsomal subfractions such as whole ribosomes, rough membranes, and smooth membranes, from healthy silkworm larvae and from those infected with cytoplasmic polyhedrosis, were prepared. Ribosomal preparations from healthy and diseased insects could not be serologically distinguished by the method of double diffusion on cellulose acetate membranes. Antisera against cytoplasmic polyhedrosis viruses appeared to share at least one antigen with the ribosomes from healthy and larvae of diseased silkworm and guinea-pig liver.


Journal of Invertebrate Pathology | 1965

Serological studies of the protein parasporal inclusions of Bacillus thuringiensis var. sotto Ishiwata

Janina Krywienczyk; T. A. Angus

Abstract The toxic parasporal protein of Bacillus thuringiensis var. sotto Ishiwata solubilized in vivo in gut juice, and in vitro under alkaline conditions, has been studied by serological methods. Antibodies elicited by alkali-dissolved protein from the crystals of Bacillus thuringiensis var. thuringiensis Berliner, Bacillus entomocidus var. entomocidus Heimpel and Angus, and B. thuringiensis var. sotto, inactivate the sotto protein in vitro and in the gut of larvae of the silkworm, Bombyx mori (linnaeus). Injections of toxin-specific antibody into the hemocoel do not protect larvae against the toxic effects of ingested crystals.


Journal of Invertebrate Pathology | 1972

Serological investigations of subcellular fractions from Malacosoma disstria larvae infected with cytoplasmic polyhedrosis

Janina Krywienczyk; Y. Hayashi

Abstract Subcellular fractions from Malacosoma disstria larvae, both healthy and infected with cytoplasmic polyhedrosis virus, were examined by double diffusion in agarose, immunoelectrophoresis, acrylamide gel electrophoresis, and combined methods. An antigen of viral origin was detected in the soluble fraction from the infected larvae. The mitochondrial fraction contained virions. Microsomal fractions showed nonspecific reactions.


Journal of Invertebrate Pathology | 1971

Specificity of serological cross-reactions between insect viruses and ribosomes

Janina Krywienczyk; Yugo Hayashi

Abstract Ribosomes from guinea pig liver, Cavia cobaya; frog liver, Rana pipiens; midgut of the silkworm, Bombyx mori; an earthworm, Lumbricus terrestris; a bacterium, Escherichia coli; a fungus of the genus Lactarius; and also the synthetic polyribonucleotides poly A and poly U were serologically compared with the cytoplasmic polyhedrosis viruses from Bombyx mori, the tent caterpillar Malacosoma disstria, and the tussock moth Orgyia leucostigma. The method of double diffusion on cellulose acetate membranes was used. Extensive cross-reactivity was observed, and it is attributed to the ribose phosphate backbone of RNA as a common antigen.


Journal of Invertebrate Pathology | 1967

Immunofluorescence studies of Bombyx mori ovarian tissue cultures infected with nuclear-polyhedrosis virus

Janina Krywienczyk; S. S. Sohi


Journal of Invertebrate Pathology | 1972

Electrophoretic fractionation of cytoplasmic polyhedrosis virus genome

Y. Hayashi; Janina Krywienczyk

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Howard T. Dulmage

United States Department of Agriculture

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Peter Lüthy

École Polytechnique Fédérale de Lausanne

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Irene Hall

University of California

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Irvin M. Hall

University of California

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Ken Y. Arakawa

University of California

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