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Dive into the research topics where Janusz Mysliwiec is active.

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Featured researches published by Janusz Mysliwiec.


Immunology Letters | 1999

γδ T-cells alterations in the peripheral blood of high risk diabetes type 1 subjects with subclinical pancreatic B-cells impairment

Adam Kretowski; Janusz Mysliwiec; Małgorzata Szelachowska; Dariusz Turowski; Jolanta Wysocka; Irina Kowalska; Ida Kinalska

There is increasing evidence that CD3 + cells bearing gammadelta T-cell receptor (represent the minor subpopulation of the T-cells in the peripheral blood in humans) are involved in autoimmunity development. Gammadelta T-cell receptor (TCR)+ /CD8+ T-cells have been recently found to play a critical role in the pathogenesis and prevention of autoimmune diabetes in the animal model. The aim of the present study was the estimation the gammadelta T-cell subpopulation levels in the peripheral blood of subjects with preclinical and overt type 1 diabetes and their possible associations with the humoral immunity, metabolic parameters and pancreatic B-cells function. The study was carried out in three groups of subjects: 26 first degree relatives of type 1 diabetes patients (prediabetics) with the combinations of autoantibodies against pancreatic B-cells (ICA, GADA, IA-2A, IAA), 22 patients with a recent onset of type 1 diabetes and age and sex-matched 24 healthy volunteers (control group). A decrease was observed in the absolute numbers and percentages of gammadelta+ /CD8+ and gammadelta+ /CD8- T-cell subpopulations in peripheral blood in the prediabetics with the impaired first phase of insulin secretion in comparison to relatives with autoantibodies but still with normal B-cells function, patients with clinical diabetes and healthy controls. In conclusion, the study suggests that the gammadelta T-cells play an important role in the development of insulin-dependent diabetes mellitus (IDDM). It is possible that their levels in the peripheral blood could be an additional marker of preclinical detection of the disease, but further prospective studies in high risk of IDDM subjects are needed.


Hormone and Metabolic Research | 2011

Estrogens modulate RANKL-RANK/osteoprotegerin mediated interleukin-6 effect on thyrotoxicosis-related bone turnover in mice.

Janusz Mysliwiec; Robert Zbucki; Agnieszka Nikolajuk; Piotr Mysliwiec; Karol A. Kamiński; Zofia Bondyra; Jacek Dadan; Maria Gorska; Maria M. Winnicka

Interleukin-6 has been shown to cause imbalance between bone resorption and formation in thyrotoxicosis. The aim of the present study was an attempt to estimate the influence of estrogens on thyrotoxicosis-related disturbances in bone turnover in relation to RANKL-RANK/osteoprotegerin system in IL-6 deficient mice. The study was performed on 56, 12-13 weeks old, female mice: C57BL/6J (wild-type; WT) and C57BL/6J (IL6-/-Kopf) (IL-6 knock-out; IL6KO). The mice were randomly divided into 8 groups with 7 mice in each one: 1. WT controls, 2. IL6KO controls, 3. WT mice with thyrotoxicosis, 4. IL6KO mice with thyrotoxicosis, 5. WT ovariectomized, 6. IL6KO ovariectomized, 7. WT ovariectomized mice with thyrotoxicosis, and 8. IL6KO ovariectomized mice with thyrotoxicosis. Experimental model of menopause was evoked by bilateral ovariectomy carried out in 8-9 weeks old mice. Thyrotoxicosis was induced by intraperitoneal injection of levothyroxine at a dose of 1 μg/g daily over 21 days. The serum levels of TRACP5b, osteocalcin, OPG, and RANKL were determined by ELISA. RANKL serum concentrations were elevated significantly in all groups of ovariectomized mice as compared to respective controls, however, in a minor degree in IL6KO thyrotoxic mice as compared to wild-type animals. Osteoprotegerin serum levels were significantly increased in all thyrotoxic groups of mice except ovariectomized IL6KO animals. To sum up, the results of the present study suggest that IL-6 plays a key role in stimulation of RANKL-RANK/OPG system and this effect is strongly enhanced in conditions of accelerated bone turnover such as thyrotoxicosis and/or estrogen depletion.


Folia Histochemica Et Cytobiologica | 2011

Role of interleukin-6 on RANKL-RANK/osteoprotegerin system in hypothyroid ovariectomized mice.

Janusz Mysliwiec; Robert Zbucki; Agnieszka Nikolajuk; Piotr Mysliwiec; Andrzej Taranta; Karol A. Kamiński; Zofia Bondyra; Jacek Dadan; Maria Gorska; Maria M. Winnicka

Postmenopausal women frequently develop hypothyroidism. Estrogen depletion is accompanied by an increase of IL-6, accelerating bone turnover. The influence of hypothyroidism on bone metabolism in postmenopausal women is poorly understood. The aim of the study was an attempt to clarify the role of interleukin-6 on RANKL-RANK/osteoprotegerin system in hypothyroid ovariectomized mice. The study was performed on 56, 12-13 weeks old, female mice: C57BL/6J (wild-type; WT) and C57BL/6JIL6-/-Kopf (IL-6 knock-out; IL6KO). The mice were randomly divided into 8 groups with 7 mice in each one: 1/ WT controls, 2/ IL6KO controls, 3/ WT hypothyroid mice, 4/ IL6KO hypothyroid mice, 5/ WT ovariectomized, 6/ IL6KO ovariectomized, 7/ WT ovariectomized hypothyroid mice and 8/ IL6KO ovariectomized hypothyroid mice. Experimental model of menopause was produced by bilateral ovariectomy carried out in 8-9 weeks old mice. Experimental model of hypothyroidism was induced by propylthiouracyl administration in driking water. The serum levels of TRACP 5b, osteocalcin, OPG and RANKL were determined by ELISA. Serum RANKL concentrations were elevated significantly in all groups of ovariectomized mice as compared to respective controls, but in a minor degree in IL6KO hypothyroid mice as compared to wild-type animals. Moreover sRANKL values were significantly lower in IL6KO as compared to WT controls and IL6KO PTU injected mice. Osteoprotegerin serum levels were decreased in all IL-6 deficient mice and in a highest degree in sham-operated hypothyroid mice. To sum up, the results of the present study suggest that estrogens deficit is a strong stimulus for RANKL-RANK/OPG pathway that breaks an inhibitory influence of hypothyroidism even in IL-6 deficient mice.


The Journal of Clinical Endocrinology and Metabolism | 2003

Intercellular Adhesion Molecule 1 Gene Polymorphisms in Graves’ Disease

Adam Kretowski; Natalia Wawrusiewicz; Katarzyna Mironczuk; Janusz Mysliwiec; Małgorzata Krętowska; Ida Kinalska


Immunological Investigations | 2007

Serum CD40/CD40L system in Graves' disease and Hashimoto's thyroiditis related to soluble Fas, FasL and humoral markers of autoimmune response.

Janusz Mysliwiec; Magdalena Okłota; Agnieszka Nikolajuk; Dariusz Waligorski; Maria Gorska


Advances in Medical Sciences | 2006

Soluble Fas, Fas ligand and Bcl-2 in autoimmune thyroid diseases: relation to humoral immune response markers

Janusz Mysliwiec; M Okota; Agnieszka Nikolajuk; Maria Gorska


Hormone and Metabolic Research | 2007

A crucial role of interleukin-6 in the pathogenesis of thyrotoxicosis-related disturbances of bone turnover in mice.

Janusz Mysliwiec; Robert Zbucki; Maria M. Winnicka; Sawicki B; Agnieszka Nikolajuk; Karol A. Kamiński; Piotr Mysliwiec; Musial W; Zofia Bondyra; Walecki J; Maria Gorska


Endokrynologia Polska | 2012

Serum interleukin-16 and RANTES during treatment of Graves′ orbitopathy with corticosteroids and teleradiotherapy

Janusz Mysliwiec; Iwona Palyga; Agnieszka Nikolajuk; Aldona Kowalska; Maria Gorska


Mediators of Inflammation | 2014

Markers of Inflammation and Fibrosis in the Orbital Fat/Connective Tissue of Patients with Graves’ Orbitopathy: Clinical Implications

Przemysław Pawłowski; Joanna Reszec; Anja Eckstein; Kristian Johnson; Andrzej Grzybowski; Lech Chyczewski; Janusz Mysliwiec


Graefes Archive for Clinical and Experimental Ophthalmology | 2014

Elevated percentage of HLA-DR⁺ and ICAM-1⁺ conjunctival epithelial cells in active Graves' orbitopathy.

Przemysław Pawłowski; Janusz Mysliwiec; Malgorzata Mrugacz; J. Zak; A. Bakunowicz-Lazarczyk; Robert Rejdak; J. Wysocka; Maria Gorska

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Maria Gorska

Medical University of Białystok

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Karol A. Kamiński

Medical University of Białystok

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Maria M. Winnicka

Medical University of Białystok

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Piotr Mysliwiec

Medical University of Białystok

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Przemysław Pawłowski

Medical University of Białystok

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Robert Zbucki

Medical University of Białystok

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Adam Kretowski

Medical University of Białystok

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Joanna Reszec

Medical University of Białystok

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