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Dive into the research topics where Jarek Baran is active.

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Featured researches published by Jarek Baran.


Archivum Immunologiae Et Therapiae Experimentalis | 2006

The migration of bone marrow-derived non-hematopoietic tissue-committed stem cells is regulated in an SDF-1-, HGF-, and LIF-dependent manner

Magda Kucia; Wojtek Wojakowski; Ryan Reca; Bogdan Machalinski; Jolanta Gozdzik; Marcin Majka; Jarek Baran; Janina Ratajczak; Mariusz Z. Ratajczak

Abstract.Introduction: Recently we identified in bone marrow (BM) by employing chemotactic isolation to SDF-1 gradient combined with real time RT-PCR analysis a mobile population of CXCR4+ BM mononuclear cells that express mRNA for various markers of early tissue-committed stem cells (TCSCs). In this study we evaluated whether TCSCs respond to other motomorphogens, such as hepatocyte growth factor (HGF) and leukemia inhibitory factor (LIF). Materials and Methods: We again employed chemotactic isolation combined with real-time RT-PCR analysis to assess whether murine and human BM contain TCSCs that respond to HGF and LIF gradients. We also evaluated expressions of HGF and LIF in damaged organs. Results: We noted that the number of TCSCs is highest in BM from young (1- to 2-month-old) mice and decreases in 1-year–old animals. Murine and human TCSCs 1) respond to HGF and LIF gradients in addition to an SDF-1 gradient, 2) reside in populations of BM-derived non-hematopoietic CD45−cells, and 3) are released (mobilized) from BM into the peripheral blood (PB) during tissue injury (e.g. after partial body irradiation). Conclusions: These findings further support our theory of the BM as a “hideout” for TCSCs and we suggest that their presence in BM tissue should be considered before experimental evidence is interpreted simply as transdifferentiation/plasticity of hematopoietic stem cells. Since we demonstrated that not only SDF-1, but also HGF and LIF are upregulated in damaged tissues, we postulate that CXCR4+ c-Met+ LIF-R+ TCSC could be mobilized from the BM into the PB, from which they are subsequently chemoattracted to damaged organs, where they play a role in tissue repair/regeneration.


International Journal of Molecular Medicine | 2015

Isolation of extracellular vesicles: Determining the correct approach (Review)

Rafał Szatanek; Jarek Baran; Maciej Siedlar; Monika Baj-Krzyworzeka

The discovery of extracellular vesicles (EVs) has revised the interpretation of intercellular communication. It is now well established that EVs play a significant role in coagulation, inflammation, cancer and stem cell renewal and expansion. Their release presents an intriguing, transporting/trafficking network of biologically active molecules, which are able to reach and modulate the function/behavior of the target cells in a variety of ways. Moreover, the presence of EVs in various body fluids points to their potential for use as biomarkers and prognostic indicators in the surveillance/monitoring of a variety of diseases. Although vast knowledge on the subject of EVs has accumulated over the years, there are still fundamental issues associated with the correct approach for their isolation. This review comprises the knowledge on EV isolation techniques that are currently available. The aim of this review was to make both experienced researchers and newcomers to the field aware that different types of EVs require unique isolation approaches. The realization of this ‘uniqueness’ is the first step in the right direction for the complete assessment of EVs.


International Journal of Molecular Sciences | 2017

The Methods of Choice for Extracellular Vesicles (EVs) Characterization

Rafał Szatanek; Monika Baj-Krzyworzeka; Jakub Zimoch; Malgorzata Lekka; Maciej Siedlar; Jarek Baran

In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been observed in many vital cellular processes including cell-to-cell communication and immune response modulation; thus, a fast and precise characterization of EVs may be relevant for both scientific and diagnostic purposes. In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization.


Journal of Translational Medicine | 2016

Colorectal cancer-derived microvesicles modulate differentiation of human monocytes to macrophages

Monika Baj-Krzyworzeka; Bożenna Mytar; Rafał Szatanek; Marcin Surmiak; Kazimierz Węglarczyk; Jarek Baran; Maciej Siedlar

BackgroundTumour-derived microvesicles (TMVs) are important players in tumour progression, modulating biological activity of immune cells e.g. lymphocytes, monocytes and macrophages. This phenomenon is particularly interesting in the progression of colon cancer, as macrophages in this type of tumour are relevant for the recovery processes. In the present study, the role of colon cancer cell-derived microvesicles in monocyte differentiation and activity profile (polarization) was investigated.MethodsMonocyte-derived macrophages (MDM) were differentiated in vitro in the presence of TMVs obtained from colon cancer: Caco-2, SW620, LoVo or SW480 cell lines and analysed according to their morphology and biological functions, as defined by cytokine secretion, reactive oxygen intermediate (ROI) production and cytotoxic activity against respective colon cancer cells.ResultsMonocytes differentiated with TMVs exhibited morphological and phenotypical characteristics of macrophages. An early contact (beginning with the first day of the in vitro culture) of monocytes with TMVs resulted in increased IL-10 secretion and only slightly elevated TNF release. Early, or prolonged contact resulted in low ROI production and low cytotoxicity against tumour cells. On the other hand, late contact of MDM with TMVs, stimulated MDM to significant TNF and IL-12 secretion, ROI production and enhanced cytotoxicity against tumour cells in vitro. In addition, differences in MDM response to TMVs from different cell lines were observed (according to cytokine secretion, ROI production and cytotoxicity against tumour cells in vitro). Biological activity, STATs phosphorylation and microRNA profiling of MDMs indicated differences in their polarization/activation status which may suggest mixed polarization type M1/M2 with the predominance of proinflammatory cells after late contact with TMVs.ConclusionsMacrophage activity (polarization status) may be regulated by contact with not only tumour cells but also with TMVs. Their final polarization status depends on the contact time, and probably on the vesicle “cargo”, as signified by the distinct impact of TMVs which enabled the switching of MDM maturation to regulatory macrophages.


Central European Journal of Immunology | 2016

Elevated level of some chemokines in plasma of gastric cancer patients

Monika Baj-Krzyworzeka; Kazimierz Węglarczyk; Jarek Baran; Antoni M. Szczepanik; Mirosław Szura; Maciej Siedlar

Introduction Gastric cancer is one of the most common cancer-related causes of death. This is mainly due to the lack of good noninvasive method/biomarkers suitable for early-tumour diagnosis and planning of further therapy modalities. Chemokines play an important role in cancer progression and metastasis formation. In gastric cancer patients, clinical relevance of CXCL12 and CCL5 level has been postulated. Aim of the study Efforts were undertaken to examine whether expanded chemokine range may be relevant for evaluation of preoperative staging of gastric cancer patients. Material and methods Plasma from 66 gastric cancer patients and 11 healthy controls was obtained, and CCL2, CCL3, CCL4, CCL5, CXCL8, CXCL9, and CXCL10 levels were determined by flow cytometry FlexSet system. Results In gastric cancer patients’ plasma an increased level of CCL2, CCL4, CCL5, CXCL8, CXCL9, and CXCL10 was observed. In the case of CCL2, CXCL9, and CXCL10, the chemokine levels correlated with advanced (III and IV in TNM classification) disease stage. In the case of CCL4, CCL5, and CXCL8, elevated levels were observed in all cancer patients in comparison to healthy donors. Conclusions The accuracy of preoperative diagnosis in gastric cancer may include the monitoring of a wide range of chemokines in patients’ plasma. Increased levels of chemokines may warn that the disease is more advanced than conventional diagnostic procedures suggest.


Archive | 2012

Application of Flow Cytometry in the Studies of Microparticles

Monika Baj-Krzyworzeka; Jarek Baran; Rafał Szatanek; Maciej Siedlar

Many cell types including leukocytes, platelets and endothelial cells release small membrane fragments called microparticles (MP). MP are shed during cell growth, activation, proliferation, senescence and apoptosis. MP contain proteins (intracellular as well as surface markers), mRNA and miRNA of the cells they have originated from. Based on the release mechanism, size and phenotype, MP are frequently divided into two categories: exosomes and ectosomes called also microvesicles. There is no doubt that the biological significance of MP has been largely overlooked for many years, regarding them as merely cellular fragments or debris. Nowadays, MP are being recognized as an important regulator of cellular interactions under physiological and pathological conditions. MP are present in all body fluids and physiologically serve various functions like blood clotting, enhance cell adhesiveness, increase cell aggregation, etc. They mediate cell-to-cell communication by transferring cell surface receptors, mRNA, and miRNA from the cell of origin to target cells. The growing body of literature regarding the role of MP in many pathologies has recently progressed from describing the association of elevated MP number with disease stage (e.g. cancer, sepsis) through understanding how MP may contribute to thrombosis, preeclampsia and tumor progression, and finally, to using MP as a source of antigens in new forms of vaccines against infectious or malignant diseases.


Oncology Letters | 2018

Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites

Bożenna Mytar; Małgorzata Stec; Rafał Szatanek; Kazimierz Węglarczyk; Katarzyna Szewczyk; Antoni M. Szczepanik; Grazyna Drabik; Jarek Baran; Maciej Siedlar; Monika Baj‑Krzyworzeka

The three cell lines, designated as gastric cancer (GC)1401, GC1415 and GC1436 were derived from peritoneal effusions from patients with gastric adenocarcinoma. Cell lines were established in tissue culture and in immunodeficient, non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. All cell lines were cultured in Dulbeccos modified Eagles medium supplemented with 5% fetal bovine serum. These cell lines were grown as an adherent monolayer with doubling time ranging between 25 h (GC1436 cell line) and 30–34 h (GC1401 and GC1415, respectively). All cells showed morphological features of epithelial-like cells, forming sheets of polygonal cells. Chromosomal analysis showed that the modal numbers ranged from 52 (GC1401), 51–56 (GC1415) and 106 (GC1436). High heterogeneity, resulting from several structural and numerical chromosomal abnormalities were evident in all cell lines. The surface marker expression suggested a tumor origin of the cells, and indicated the intestinal phenotype of a GC (CD10+, MUC1). All three cell lines were tumorigenic but not metastatic, in vivo, in NOD/SCID mice. The lack of metastatic potential was suggested by the lack of aldehyde dehydrogenase 1A1 activity. In conclusion, these newly established GC cell lines widen the feasibility of the functional studies on biology of GC as well as drug testing for potential therapeutic purposes.


Blood | 2004

Mobilization Studies in Mice Deficient in Either C3 or C3a-Receptor (C3aR) Reveal a Novel Role For Complement in Retention of Hematopoietic Stem/Progenitor Cells in Bone Marrow

Janina Ratajczak; Ryan Reca; Magda Kucia; Marcin Majka; Daniel J. Allendorf; Jarek Baran; Anna Janowska-Wieczorek; Rick A. Wetsel; Gordon D. Ross; Mariusz Z. Ratajczak


Cancer Research | 2003

β-Glucan Functions as an Adjuvant for Monoclonal Antibody Immunotherapy by Recruiting Tumoricidal Granulocytes as Killer Cells

Feng Hong; Richard Hansen; Jun Yan; Daniel J. Allendorf; Jarek Baran; Gary R. Ostroff; Gordon D. Ross


Blood | 2006

-Glucan enhances complement-mediated hematopoietic recovery after bone marrow injury

Daniel E. Cramer; Daniel J. Allendorf; Jarek Baran; Richard Hansen; Jose Marroquin; Bing Li; Janina Ratajczak; Mariusz Z. Ratajczak; Jun Yan

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Maciej Siedlar

Jagiellonian University Medical College

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Rafał Szatanek

Jagiellonian University Medical College

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Kazimierz Węglarczyk

Jagiellonian University Medical College

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Antoni M. Szczepanik

Jagiellonian University Medical College

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Bożenna Mytar

Jagiellonian University Medical College

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Marcin Majka

Jagiellonian University Medical College

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