Jason Condon

Three long-term trials end with a quasi-equilibrium between soil C, N, and pH: an implication for C sequestration

The aim of this study was to assess the long-term changes in some key soil chemical properties at the completion of three long-term trials in south-eastern Australia and the relationship between those soil properties. From a soil organic matter perspective, the build-up of carbon (%C) requires an accumulation of nitrogen (%N), and the build-up of %C and %N fertility comes at the cost of soil acidity. Rotation, tillage, and stubble practices combine to alter the quantity, quality (C : N), and the depth distribution of organic matter in a soil, but the three soil chemical properties reported here seem to also be in quasi-equilibrium at the three long-term sites. The consequence is that if the build-up of soil organic matter leads to soil acidification, then the maintenance of agricultural production will require liming. The emission of CO2 when limestone reacts with soil acids, plus the C cost of limestone application, will negate a proportion of the gains from C sequestration as organic matter in soil. Such cautionary information was doubtless unforeseen when these three long-term trials were initiated.

New fertiliser options for managing phosphorus for organic and low-input farming systems

Plant-available phosphorus (P) has been found to be limiting crop and pasture production in Australian dryland, broadacre, organic farming systems. The present review examines the mechanisms that act to provide organic sources of P to soil or mobilise P stored within the soil. A range of products is available to exploit one or more of these mechanisms to achieve a claimed improvement in P fertility. These products are described, and where possible, scientific research of their effectiveness is reviewed. The use of microbial inoculants, although successful in laboratory and glasshouse experiments, has returned varied results in field trials. The addition of organic fertilisers, such as composted or elemental sulfur (S) enriched reactive phosphate rock (RPR), tended to produce more reliable results. The variable nature of the composting process creates complexity in the production of composted RPR. The increased dissolution of RPR by the oxidation of added S has been successful in increasing available P content above that of RPR alone. This is especially significant to low-rainfall areas where RPR tend to be ineffective. This paper highlights the need for development and optimisation of the many organic fertilisers and additives available to organic producers. In all cases, products still require rigorous field and economic evaluation so that organic producers can be confident in making decisions that are informed, correct, and profitable with regard to P fertility. The alleviation of P deficiency is vital to the increased adoption and sustainability of boardacre organic farming in Australia.

The role of N transformations in the formation of acidic subsurface layers in stock urine patches

This study examines the role of nitrogen transformations in the acidification of soil under stock urine patches, specifically the formation of acidic subsurface layers. These are horizontal planes of acidity several centimetres below the soil surface. Glasshouse studies were conducted to relate nitrogen transformations to measured pH changes in soil treated with urine or urea solution (simulated urine). Acidic subsurface layers formed in both urine- and simulated urine-treated soil. With the development of a H + balance model, the contribution of nitrogen transformations to changes in the H + concentrations in simulated urine patches was determined. During the first 9 days following treatment, urea hydrolysis and NH3 volatilisation dominated changes in H + concentration. After that, net immobilisation contributed to H + changes; however, nitrification was the dominant process occurring. Downward movement of NH4 + originating from urea hydrolysis allowed more nitrification to occur in lower soil layers. The net result of these processes was net acidification of the 4-6, 6-8, and 8-10 cm layers by approximately 0.7, 0.6, and 0.3 pH units, respectively. Thus nitrogen transformations were responsible for the formation of acidic subsurface layers in simulated stock urine patches within 6 weeks of application. SR03 J R. et al Aci ace st tc Additional keywords: acidification, soil pH, urea, nitrification, H + budget.

Uptake of water from a Kandosol subsoil. II. Control of water uptake by roots

AimTo test for the presence of an impediment to water flow at the soil-root interface.MethodsWheat plants were grown in repacked and undisturbed field soil. Their transpiration rate, E, was varied in several steps from low to high and then back to low again, while the hydrostatic pressure in the leaf xylem, ψxylem, was measured non-destructively and continuously. These measurements were compared to a mathematical model that calculated ψxylem by assuming that the hydraulic resistance across the plant was constant and that the radial flow of water to unit length of a typical plant root generated gradients in pressure in the soil water.ResultsFor the repacked soil, the radial flow model could not match the experiment during the falling phase of E, unless it was assumed that either an additional, constant, interfacial resistance between the soil and the roots had developed when E was large and ψxylem was rapidly falling, or that the resistance within the plant had changed. For the undisturbed field soil, the radial flow model did not agree with the experiment. Plausible agreement was achieved when plant water uptake was accounted for using a distributed sink model in HYDRUS-1D, with E integrated across the rootzone. This approach was based on the measured large variation in the vertical distribution of roots.ConclusionsThere was no strong evidence of large drawdowns of soil water in the rhizosphere, even when ψxylem was falling rapidly when E was large and the soil was moderately dry. Thus, there seems to have been an additional impediment to water flow from soil to plant, either within the plant, or at the interface between the two.

Parent material and climate affect soil organic carbon fractions under pastures in south-eastern Australia

In the present field survey, 72 sites were sampled to assess the effect of climate (Monaro, Boorowa and Coleambally regions) and parent material (Monaro region only; basalt and granite) on soil organic carbon (OC) under perennial pastures. In the higher-rainfall zone (Monaro and Boorowa; >500mm mean annual rainfall), OC stocks under introduced and native perennial pastures were compared, whereas in the lower-rainfall zone (Coleambally; <500mm mean annual rainfall) OC stocks under crops and pastures were compared. Carbon fractions included total OC (TOC), particulate OC (POC), resistant OC (ROC) and humic OC (HUM). Higher OC stocks were associated with higher spring and summer rainfall and lower annual temperatures. Within a climatic zone, parent material affected the stock of OC fractions in the 0–30cm soil layer. Within a climatic zone, when grouped by parent material, there was no difference in OC stock with vegetation type. There were significant correlations between soil factors associated with parent material and OC concentration, including negative correlations between SiO2 and HUM (P<0.05) and positive correlations between cation exchange capacity and TOC, POC and ROC (P<0.01). TOC was also positively correlated with total nitrogen (N) and available sulfur (S; P<0.05), indicating organic matter in soil is important for N and S supply for plant production in the studied regions, and vice versa. Although ensuring adequate available S may increase OC stocks in south-eastern Australia, the large stock of OC in the soil under perennial pastures, and the dominating effect of climate and parent material on this stock, may mean that modest increases in soil OC due to management factors go undetected.

Effect of VRN1 and PPD1 genes on anthesis date and wheat growth

Abstract. Phasic development of wheat is largely determined by the interaction of the VRN1 and PPD1 genes with vernalising temperature and photoperiod. VRN1 and PPD1 are regulatory genes, known to influence freezing tolerance, plant morphology and grain yield as well as phasic development. Forty-seven doubled-haploid lines were characterised for Ppd-B1, Ppd-D1, Vrn-A1, Vrn-B1 and Vrn-D1 to determine the effect of allelic combinations of these genes on timing of anthesis and crop growth rate. The lines were grown in replicated field experiments at two locations in Australia. The VRN1 and PPD1 genes accounted for 75% of the genetic variation for time from sowing to anthesis. Vrn-A1 and Vrn-B1 similarly affected time to anthesis, but only Vrn-B1 affected crop growth rate, with the spring Vrn-B1a allele resulting in faster crop growth rates than the winter Vrn-B1v allele. This suggests that the effect of Vrn-B1 on crop growth rate is not a direct consequence of its effect on development per se, but rather due to its influence on other physiological processes. The faster growth associated with Vrn-B1a may explain the high grain yield of cultivars with this allele in some environments, as shown in a previous study.

Rapid analysis of the nitrification inhibitor 3,4-dimethylpyrazole phosphate in soil using LC-MS/MS

ABSTRACT Nitrate from the biological nitrification of ammonium fertilisers causes environmental damage via groundwater contamination and nitrous oxide emission. To limit nitrate formation, nitrification inhibitors (NIs) are used in conjunction with ammonium-based fertilisers in agricultural land management. The NI 3,4-dimethyl-1H-pyrazole phosphate (DMPP), with an active constituent 3,4-dimethyl-1H-pyrazole (3,4-DMP), is commercially available and its effectiveness and behaviour in soils have been studied. However, only one method for the analysis of 3,4-DMP in soil has been reported and relies on extensive sample preparation to remove matrix interferences prior to HPLC analysis. A new method was developed to allow monitoring of 3,4-DMP residues in soil after appliaction, which utilises the greater selectivity and sensitivity of LC-MS/MS. A 3,4-DMP limit of quantitation of 0.5 ng/g was achieved, which is 10 times more sensitive than the published method, and was achieved using 10,000 times less 3,4-DMP injected on-column, with an injection volume 100 times smaller. Four internal standards were evaluated to improve the accuracy of the extraction method. The isotope-substituted structural isomer 3,5-dimethyl pyrazole-15N2 provided the best and most consistent recoveries over the 300-fold concentration range tested. The new method was employed to investigate the persistence and mobility of 3,4-DMP in an agricultural soil. 3,4-DMP had a half-life of 5 days in the top 0.5 cm of soil at normal and double recommended application rates, while half-lives in the 2.5 cm soil profile were 28 and 21 days, respectively. 3,4-DMP mobility in the clay loam soil tested was low, with only 15–25% of applied 3,4-DMP detected below the top 0.5 cm, suggesting the loss of 3,4-DMP was either due to volatilisation or degradation, rather than leaching into the soil profile.

The influence of potassium and defoliation of ryegrass on the formation of acidic subsurface layers in stock urine patches

This study examined the influence of simulated urine solutions containing various KCl and urea-N rates on the formation of acidic subsurface layers in soil columns. A factorial design was implemented with application rates equivalent to 0, 21, 42, 63, and 84 g urea-N/m2 and 0, 12.5, 25, and 37.5 g KCl-K/m2. The addition of N caused the formation of acidic subsurface layers at depths between 0.02 and 0.10 m. The magnitude of the resultant net acidification and the depth of the most acidic layer increased with N rate. More acidification occurred at depth at the higher N rates due to the downward movement of NH4+-N. The inclusion of K in the simulated urine produced less acidity in the surface layers and more acidity at depth as the K application rate increased owing to competition between K+ and NH4+-N for exchange sites, allowing more NH4+-N to move to depth. The residual acidity in the soil at the completion of the experiment was found to be greater than the alkalinity of plant material. Therefore, acidic subsurface layers are likely to persist after plant death and decomposition. We also examined the impact of defoliation on the resultant pH profiles formed following simulated urine addition. Defoliation accentuated the magnitude of acidic subsurface layers, possibly due to changes in the rate of N uptake. The influence of defoliation was minor compared with the main effects of N addition.