Jaydeep Halder

Biotechnological Advancements and Begomovirus Management in Okra (Abelmoschus esculentus L.): Status and Perspectives

Despite the importance of okra, as one of the important vegetable crop, very little attention has been paid to its genetic improvement using advanced biotechnological tools. The exploitation of marker assisted breeding in okra is often limited due to the availability of a few molecular markers, the absence of molecular genetic-map(s), and other molecular tools. Chromosome linkage-groups were not yet constructed for this crop and reports on marker development are very scanty and mostly hovering around cultivar characterization. Besides, very little progress has been observed for transgenic development. However, high throughput biotechnological tools like chromosome engineering, RNA interference (RNAi), marker-assisted recurrent selection (MARS), genome-wide selection (GWS), targeted gene replacement, next generation sequencing (NGS), and nanobiotechnology can provide a rapid way for okra improvement. Further, the etiology of many deadly viral diseases like the yellow vein mosaic virus (YVMV) and okra enation leaf curl virus (OELCV) in okra is broadly indistinct and has been shown to be caused by various begomovirus species. These diseases cause systemic infections and have a very effective mode of transmission; thus, preventing their spread has been very complicated. Biotechnological interventions have the potential to enhance okra production even under different viral-stress conditions. In this background, this review deals with the biotechnological advancements in okra per se along with the begomoviruses infecting okra, and special emphasis has been laid on the exploitation of advanced genomic tools for the development of resistant varieties.

Perceived Effectiveness of Indigenous Technological Knowledge (ITK) of Insect and Vertebrate Pests Management in Eastern Uttar Pradesh, India

Abstract Indigenous technological knowledge is considered as a traditional knowledge that inherited from their ancestors, unique to a culture, society and environment. In this article we documented uses of such local knowledge used by the local farmers of seven villages form Varanasi, Deoria and Mirzapur districts of eastern Uttar Pradesh, India for their crop protection. Data were collected from 140 respondents (twenty from each village) and 60 respondents who had visited the institute from these three districts during farmers’ fair and farmers’ training and whole making a total of 200 respondents. The farmers in the study developed notable innovations to control major pests of agricultural and horticultural crops such as use of ashes to control vegetable insect pests; use of onion/garlic bulbs, neem leaves, salt crystals, wheat straw, brick kiln powder etc. to prevent store grain pests of cereals; ash and kerosene mixture to repel the sucking pests of rice at milky stage; well-rotted fish scales against Nilgai (Boselaphus tragocamelus); red chilli pods against bruchids in pulses; salt and turmeric powder mixture against ants; use of neem cake to repel termite and other soil insects etc. The local farmers were following these practices because of easy availability, eco-friendly, easy to handle, do not leave any harmful residue, pose any threat to develop resistance and resurgence of secondary pests, easy to apply and compatible with other pest management techniques and socio-cultural situation of the farmers.