Jayesh P. Ruparelia

Strain specificity in antimicrobial activity of silver and copper nanoparticles.

The antimicrobial properties of silver and copper nanoparticles were investigated using Escherichia coli (four strains), Bacillus subtilis and Staphylococcus aureus (three strains). The average sizes of the silver and copper nanoparticles were 3 nm and 9 nm, respectively, as determined through transmission electron microscopy. Energy-dispersive X-ray spectra of silver and copper nanoparticles revealed that while silver was in its pure form, an oxide layer existed on the copper nanoparticles. The bactericidal effect of silver and copper nanoparticles were compared based on diameter of inhibition zone in disk diffusion tests and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of nanoparticles dispersed in batch cultures. Bacterial sensitivity to nanoparticles was found to vary depending on the microbial species. Disk diffusion studies with E. coli and S. aureus revealed greater effectiveness of the silver nanoparticles compared to the copper nanoparticles. B. subtilis depicted the highest sensitivity to nanoparticles compared to the other strains and was more adversely affected by the copper nanoparticles. Good correlation was observed between MIC and MBC (r2=0.98) measured in liquid cultures. For copper nanoparticles a good negative correlation was observed between the inhibition zone observed in disk diffusion test and MIC/MBC determined based on liquid cultures with the various strains (r2=-0.75). Although strain-specific variation in MIC/MBC was negligible for S. aureus, some strain-specific variation was observed for E. coli.

Electrocoagulation treatment of simulated floor-wash containing Reactive Black 5 using iron sacrificial anode.

Floor-wash from dye finishing plant is a major source of color and wastewater volume for dyes industries. Batch electrocoagulation (EC) of simulated floor-wash containing Reactive Black 5 (RB5) was studied as a possible pretreatment option. More than 90% of initial 25mg/L of RB5 was removed at current densities of 4.5, 6, and 7.5 mA/cm(2) in the presence of Na(2)SO(4) and NaCl as supporting electrolytes; in less than one hour. Identical k(obs) (pseudo first-order reaction rate constant) values were obtained at initial pH of 3.74 for both electrolytes. However, at initial pH of 6.6, k(obs) values decreased in the presence of Na(2)SO(4) and remained same for NaCl as compared to that at pH 3.74. Highest extent of decolorization and k(obs) values were obtained at initial pH 9.0 for both electrolytes. Under identical conditions, specific energy consumption (SEC) was almost half in the presence of NaCl (~29 kWh/kg RB5) than that of Na(2)SO(4). Vinyl sulfone (VS) was detected as one of the products of EC indicating reduction of azo bonds as a preliminary step of decolorization. Mechanism of decolorization with respect to various experimental conditions was delineated. Generation and accumulation of VS was dependent on initial pH and type of electrolyte. Results of this study revealed that EC in the presence of sodium chloride can be efficiently used as a primary treatment for decolorization of floor-wash containing RB5.

Antimicrobial Activity of Silver and Copper Nanoparticles: Variation in Sensitivity Across Various Strains of Bacteria and Fungi

The antimicrobial activity of silver and copper nanoparticles is widely reported and is linked with ions that leach out from these nanoparticles. The activity is further enhanced due to their small size and high surface area to volume ratio which allows them to interact closely with microbial membranes. Most studies on antibacterial effects have been limited to one or a few strains and comparison across studies becomes difficult due to differences in the size and other characteristics of the nanoparticles and due to differences in the protocols followed in the various studies. The sensitivity in response to silver nanoparticles is seen to vary widely across various strains of Escherichia coli and Staphylococcus aureus. Most strains typically show greater sensitivity to silver compared to copper nanoparticles. Antifungal activity of silver nanoparticles has been found to be comparable to commercially available antifungal agents. Nanoparticles embedded/immobilized on supports may be better utilized for applications such as water disinfection. Such systems can promote a continuous release of Ag+ and Cu2+ ions in solution and thus promote disinfection while ensuring a low enough concentration to avoid deleterious effect on humans and other organisms in the ecosystem.

Assessing groundwater vulnerability using GIS-based DRASTIC model for Ahmedabad district, India

The present research aims to derive the intrinsic vulnerability of groundwater against contamination using the GIS platform. The study applies DRASTIC model for Ahmedabad district in Gujarat, India. The model uses parameters like depth, recharge, aquifer, soil, topography, vadose zone and hydraulic conductivity, which depict the hydrogeology of the area. The research demonstrates that northern part of district with 46.4% of area is under low vulnerability, the central and southern parts with 48.4% of the area are under moderate vulnerability, while 5.2% of area in the south-east of district is under high vulnerability. It is observed from the study that lower vulnerability in northern part may be mostly due to the greater depth of vadose zone, deeper water tables and alluvial aquifer system with minor clay lenses. The moderate and high vulnerability in central and southern parts of study area may be due to lesser depth to water tables, smaller vadose zone depths, unconfined to semi-confined alluvial aquifer system and greater amount of recharge due to irrigation practices. Further, the map removal and single-parameter sensitivity analysis indicate that groundwater vulnerability index has higher influence of vadose zone, recharge, depth and aquifer parameters for the given study area. The research also contributes to validating the existence of higher concentrations of contaminants/indicators like electrical conductivity, chloride, total dissolved solids, sulphate, nitrate, calcium, sodium and magnesium with respect to groundwater vulnerability status in the study area. The contaminants/indicators exceeding the prescribed limits for drinking water as per Indian Standard 10500 (1991) were mostly found in areas under moderate and high vulnerability. Finally, the research successfully delineates the groundwater vulnerability in the region which can aid land-use policies and norms for activities related to recharge and seepage with respect to existing status of groundwater vulnerability and its quality.