Jean-Christophe Cau
University of Toulouse
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Publication
Featured researches published by Jean-Christophe Cau.
Langmuir | 2009
Aline Cerf; Jean-Christophe Cau; Christophe Vieu; Etienne Dague
The main goal of this paper is to probe mechanical properties of living and dead bacteria via atomic force microscopy (AFM) indentation experimentations. Nevertheless, the prerequisite for bioAFM study is the adhesion of the biological sample on a surface. Although AFM has now been used in microbiology for 20 years, the immobilization of micro-organisms is still challenging. Immobilizing a single cell, without the need for chemical fixation has therefore constituted our second purpose. Highly ordered arrays of single living bacteria were generated over the millimeter scale by selective adsorption of bacteria onto micrometric chemical patterns. The chemically engineered template surfaces were prepared with a microcontact printing process, and different functionalizations of the patterns by incubation were investigated. Thanks to this original immobilization strategy, the Young moduli of the same cell were measured using force spectroscopy before and after heating (45 degrees C, 20 min). The cells with a damaged membrane (after heating) present a Young modulus twice as high as that of healthy bacteria.
Colloids and Surfaces B: Biointerfaces | 2008
Aline Cerf; Jean-Christophe Cau; Christophe Vieu
Highly ordered arrays of single living bacteria were obtained by selective adsorption of bacteria onto chemical patterns with micrometric resolution. The chemically engineered template surfaces were prepared with the combination of microcontact printing process and a simple incubation technique. This methodology can be used for fundamental studies of bacteriums inner mechanisms and sub-cellular organization as well as for interfacing living bacteria with artificial microsystems.
Oncologie | 2009
Jean-Christophe Cau; H. Lalo; Childerick Severac; Jean-Pierre Peyrade; Emmanuelle Trévisiol; Véronique Anton Leberre; Jean François; Christophe Vieu
RésuméDans ce travail nous montrons que la structuration à l’échelle nanométrique de biomolécules sondes par lithographie douce permet de fabriquer des puces à protéines à un coût de production suffisamment réduit pour entrevoir leur utilisation dans le domaine de l’analysemoléculaire médicale. La combinaison d’un procédé d’impression moléculaire et d’une détection optique sans marquage fondée sur le principe de la diffraction de la lumière est mise en oeuvre afin de produire des supports d’analyse en verre comportant des motifs nanométriques et un scanner de diffraction qui permet la lecture d’un test biologique multiplexé.AbstractIn this article, we show that by biopatterning probe molecules at the nanoscale using soft lithography, protein biochips can be produced at a significantly lower cost for their use as a systematic method of molecular analysis for medical diagnosis purposes. The combination of multiplexed nanoscale microcontact printing and label-free optical detection using the principle of light diffraction is implemented for generating engineered glass slides for analysis, and a dedicated diffractive scanner for reading the multiplexed results of an assay.
Microarrays | 2016
Julie Fredonnet; Julie Foncy; Jean-Christophe Cau; Childérick Séverac; Jean François; Emmanuelle Trévisiol
Microarrays are established research tools for genotyping, expression profiling, or molecular diagnostics in which DNA molecules are precisely addressed to the surface of a solid support. This study assesses the fabrication of low-density oligonucleotide arrays using an automated microcontact printing device, the InnoStamp 40®. This automate allows a multiplexed deposition of oligoprobes on a functionalized surface by the use of a MacroStampTM bearing 64 individual pillars each mounted with 50 circular micropatterns (spots) of 160 µm diameter at 320 µm pitch. Reliability and reuse of the MacroStampTM were shown to be fast and robust by a simple washing step in 96% ethanol. The low-density microarrays printed on either epoxysilane or dendrimer-functionalized slides (DendriSlides) showed excellent hybridization response with complementary sequences at unusual low probe and target concentrations, since the actual probe density immobilized by this technology was at least 10-fold lower than with the conventional mechanical spotting. In addition, we found a comparable hybridization response in terms of fluorescence intensity between spotted and printed oligoarrays with a 1 nM complementary target by using a 50-fold lower probe concentration to produce the oligoarrays by the microcontact printing method. Taken together, our results lend support to the potential development of this multiplexed microcontact printing technology employing soft lithography as an alternative, cost-competitive tool for fabrication of low-density DNA microarrays.
PLOS ONE | 2018
Julie Foncy; Aurore Estève; Amélie Degache; Camille Colin; Xavier Dollat; Jean-Christophe Cau; Christophe Vieu; Emmanuelle Trévisiol; Laurent Malaquin
Microcontact printing has become a versatile soft lithography technique used to produce molecular micro- and nano-patterns consisting of a large range of different biomolecules. Despite intensive research over the last decade and numerous applications in the fields of biosensors, microarrays and biomedical applications, the large-scale implementation of microcontact printing is still an issue. It is hindered by the stamp-inking step that is critical to ensure a reproducible and uniform transfer of inked molecules over large areas. This is particularly important when addressing application such as cell microarray manufacturing, which are currently used for a wide range of analytical and pharmaceutical applications. In this paper, we present a large-scale and multiplexed microcontact printing process of extracellular matrix proteins for the fabrication of cell microarrays. We have developed a microfluidic inking approach combined with a magnetic clamping technology that can be adapted to most standard substrates used in biology. We have demonstrated a significant improvement of homogeneity of printed protein patterns on surfaces larger than 1 cm2 through the control of both the flow rate and the wetting mechanism of the stamp surface during microfluidic inking. Thanks to the reproducibility and integration capabilities provided by microfluidics, we have achieved the printing of three different adhesion proteins in one-step transfer. Selective cell adhesion and cell shape adaptation on the produced patterns were observed, showing the suitability of this approach for producing on-demand large-scale cell microarrays.
Microelectronic Engineering | 2009
Helene Lalo; Jean-Christophe Cau; Christophe Thibault; Nathalie Marsaud; Childerick Severac; Christophe Vieu
Microelectronic Engineering | 2013
Jean-Christophe Cau; Lafforgue Ludovic; Nogues Marie; Lagraulet Adriana; Paveau Vincent
Microelectronic Engineering | 2008
Jean-Christophe Cau; Aline Cerf; Christophe Thibault; Mike Geneviève; Childerick Severac; Jean-Pierre Peyrade; Christophe Vieu
Microelectronic Engineering | 2013
Julie Fredonnet; Julie Foncy; Sophie Lamarre; Jean-Christophe Cau; Emmanuelle Trévisiol; Jean-Pierre Peyrade; Jean François; Childérick Séverac
Microelectronic Engineering | 2013
Julie Foncy; Jean-Christophe Cau; Carlos Bartual-Murgui; Jean François; Emmanuelle Trévisiol; Childerick Severac