Jean-Francois Stumbe

Dendritic Polyamines: Simple Access to New Materials with Defined Treelike Structures for Application in Nonviral Gene Delivery

Polycationic dendrimers are interesting nonviral vectors for in vitro DNA delivery. We describe a simple approach to the synthesis of dendritic polyamines with different molecular weights and adjustable flexibility (degrees of branching; DB). Both parameters influence the transfection efficiency and the cell toxicity of the polymer. Functionalization of hyperbranched polyethylenimine (PEI) by a two‐step procedure generated fully branched pseudodendrimers (analogues of polypropylenimine (PPI) and polyamidoamine (PAMAM) dendrimers). The DNA transfection efficiencies observed for these polymers depended on the cell line investigated. The highest efficiencies were observed for polymers whose unfunctionalized PEI cores had molecular weights in the range Mw=6000–25 000 g mol−1. The cytotoxicity of the dendrimers generally rises with increasing core size. The data collected for NIH/3T3 and COS‐7 cells indicate a maximum transfection efficiency at around 60 % branching for the PPI analogues, and at a PEI‐core molecular weight of Mw=25 000 g mol−1. PAMAM functionalization of PEI (Mw=5000 and 21 000 g mol−1) leads to polymers with little or no cytotoxity in the cell lines investigated.

Light-Powered Self-Healable Metallosupramolecular Soft Actuators

Supramolecular functional materials able to respond to external stimuli have several advantages over their classical covalent counterparts. The preparation of soft actuators with the ability to respond to external stimuli in a spatiotemporal fashion, to self-repair, and to show directional motion, is currently one of the most challenging research goals. Herein, we report a series of metallopolymers based on zinc(II)-terpyridine coordination nodes and bearing photoisomerizable diazobenzene units and/or solubilizing luminescent phenylene-ethynylene moieties. These supramolecular polymers act as powerful gelating agents at low critical gelation concentrations. The resulting multiresponsive organogels display light-triggered mechanical actuation and luminescent properties. Furthermore, owing to the presence of dynamic coordinating bonds, they show self-healing abilities.

Polyester-Based, Biodegradable Core-Multishell Nanocarriers for the Transport of Hydrophobic Drugs

A water-soluble, core-multishell (CMS) nanocarrier based on a new hyperbranched polyester core building block was synthesized and characterized towards drug transport and degradation of the nanocarrier. The hydrophobic drug dexamethasone was encapsulated and the enzyme-mediated biodegradability was investigated by NMR spectroscopy. The new CMS nanocarrier can transport one molecule of dexamethasone and degrades within five days at a skin temperature of 32 °C to biocompatible fragments.