Jean-Louis Funck-Brentano

Radioactive microsphere distribution and single glomerular blood flow in the normal rabbit kidney

SummaryIntracortical distribution of blood flow was studied in the rabbit kidney with 15 μm labelled microspheres (M) injected into the left ventricule. M injection did not alter renal function. Thanks to arterial filling of left kidney with silicone rubber, efferent vascular patterns of the glomeruli could be precisely identified. Glomeruli of different populations were sampled by microdissection and their radioactivity measured. Assuming that intracortical distribution of M reflected distribution of flow to the glomeruli, individual glomerular blood flows (GBF) were determined. In hydropenic rabbits, GBF was higher in deep glomeruli providing vasa recta (G4) (193±14 nl·min−1) than in most other superficial (G1 and G2) and deep glomeruli (G3) (190±27, 113±10 and 127±9 nl·min−1 respectively;

Methods for measurement of renal blood flow in man

\bar m \pm SEM, n = 6)

Electrophoretical characterization of red blood cell membrane proteins and plasma protein in spontaneously hypertensive rat

. The exact significance of GBF found in superficial glomeruli with straight ascending efferent arterioles supplying aglomerular suscapsular cortex (G1) was questioned because of the possible axial streaming of spheres. Afferent medulary blood flow was calculated to represent 9.0±0.9% of total renal blood flow.

Plasma vitamin D metabolites in a patient with sporadic hypophosphataemic osteomalacia (adult-onset type).

Abstract The 25-hydroxycholecalciferol-binding protein has been partially purified (purification factor: 37) from rat duodenal cytosol, using chromatographic procedures on gel and ionic exchange resin. This partially purified protein bound 25-hydroxycholecalciferol with high affinity (KD = 5.7 × 10−9M) and low binding capacity (23 × 10−12 mole/mg of protein. Using a rabbit antiserum obtained against such partially purified protein, we demonstrated that 25-hydroxycholecalciferol cytosolic binder and 25-hydroxycholecalciferol plasmatic binding share common antigenic sites.

The Polyacrylonitrile Membrane and the Rhodial System: Their Practical Application

Methods currently available for measurement of renal blood flow with radio-nuclides in man include clearance-extraction ratio, indicator dilution, and inert-gas washout. Only the gas washout method, involving 133 Xe or 85 Kr, provides data on intrarenal distribution of blood flow; it also permits the measurement of renal blood flow of individual kidneys and may be used in oligoanuric patients. It necessitates selective catheterization of the renal artery and external counting. The gas washout technique represents to date the most satisfactory method of studying renal hemodynamics in man. However, several problems remain unresolved, among which are the assumption of instantaneous gas diffusion in any normal or pathological state, the effects of the intra-arterial bolus injection, the exact significance of the second component of the washout curve, and the value of data obtained by computer analysis of the curves.

In vitro relative biological activities of (1--34)N-terminal synthetic fragments of human parathyroid hormone in the human renal cortical adenylate cyclase assay.

Abstract 1α, 25-Dihydroxycholecalciferol (1,25-(OH) 2 D 3 ) and 25-hydroxycholecalciferol (25-(OH)D 3 ) nuclear uptake was in vitro studied in duodenal mucosa cells from vitamin D-deficient rats. Both 1,25-(OH) 2 D 3 and 25-(OH)D 3 were found in association with purified nuclei. However a saturable maximal uptake (1.4 pmol/mg DNA) was demonstrated only for 1,25-(OH) 2 D 3 . This 1,25-(OH) 2 D 3 nuclear uptake is specific as it is not altered by the presence of either 25-(OH)D 3 or 1α-hydroxycholecalciferol.

Liquid scintillation counting for measurement of tritiated uptake in lymphocyte transformation in vitro: A new direct-suspension procedure

Abstract The red blood cell membrane proteins and plasma proteins of normal and spontaneously hypertensive rats were studied by uni- and bidimensional polyacrylamide gel electrophoresis. The amount of band 3, the major intrinsic protein of the erythrocyte membrane, was observed to be significantly reduced in spontaneously hypertensive rats. Plasma from these rats contained two additional heat stable proteins, characterized by a molecular weight of 16,000 daltons and isoelectric points of 4.7 and 5.1, respectively. These proteins were not detected in normotensive control Wistar Kyoto rats, in normal Wistar rats, or in Wistar rats with experimentally induced hypertension.

The Polyacrylonitrile Membrane; Use in Dialysis with the Rhodial System. Use in Haemofiltration

Abstract. Sporadic hypophosphataemic osteomalacia (adult‐onset type) was demonstrated in a 40‐year‐old man on the basis of severe osteomalacia, hypophos‐phataemia, hyperphosphaturia and glycinuria. Plasma immunoreactive parathyroid hormone (iPTH) concentration was 9‐3 ng prot./ml (normal range: 4–8 ng prot./ml). Plasma 25‐hydroxy‐vitamin D and 24, 25‐dihydroxy‐vitamin D concentrations were 11 and 2–4 ng/ml respectively. Basal lα, 25‐dihydroxy‐vitamin D concentrations were slightly elevated (116 and 96 pg/ml) and increased to 240 pg/ml after 3 days on a low‐phosphorus diet. The patient was put on oral treatment with 25‐hydroxycholecalciferol (100 μ per day) and phosphorus (1500 mg per day). On the 4th month on treatment, a clinical improvement was apparent. Plasma 25(OH) D was 44 ng/ml, plasma l,25 (OH)2D was 256 pg/ml. However, plasma phosphorus remained low (0.77 mmol/l). On the 9th month of treatment a radiological improvement was evident despite a persistent hypophosphataemia (0.68 mmol/I). These facts suggest in our patient the existence of a vitamin D‐independent renal phosphorus leak.