Jeanine M. Davis

[14C]-Assimilate translocation in the light and dark in celery (Apium graveokns) leaves of different ages

The 2 major photosynthetic products and translocated carbohydrates in celery (Apium graveolens L.) are sucrose and the sugar alcohol, mannitol. Sucrose is produced and utilized in leaves of all ages. Mannitol, however, is synthesized primarily in mature leaves, utilized in young leaves and stored in all leaves. Here we show that mannitol export was lower from young, expanding leaves than from older leaves. After a 10 min pulse of (14) CO(2) and a 2 h chase in the light or dark there was more radioactivity in sucrose than in mannitol in petiole tissues from leaves of all ages. However, after a chase of 15 h in the dark or 6 h in the light followed by 9 h in the dark, mannitol was the predominant [(14) C]-labeled carbohydrate remaining in all leaf and petiole tissues. Thus, newly synthesized sucrose was apparently exported at a faster rate than mannitol and more mannitol was partitioned into vacuolar storage pools than was sucrose. It also appears that in the light both sucrose and mannitol were exported, but in the dark, once sucrose pools were depleted, mannitol remained as the predominant substance translocated. Both mannitol and sucrose were unloaded into petiole storage parenchyma tissue, but sucrose was hydrolyzed prior to storage.

Echinacea and its alkylamides: effects on the influenza A-induced secretion of cytokines, chemokines, and PGE2 from RAW 264.7 macrophage-like cells.

The goal of this study was to determine whether extracts and isolated alkylamides from Echinacea purpurea would be useful for prevention of the inflammatory response that accompanies infections with H1N1 influenza A. Seventeen extracts and 4 alkylamides were tested for the ability to inhibit production of cytokines, chemokines, and PGE₂ from RAW 264.7 macrophage-like cells infected with the H1N1 influenza A strain PR/8/34. The alkylamides undeca-2Z,4E-diene-8,10-diynic acid isobutylamide, dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide, dodeca-2E,4E-dienoic acid isobutylamide, and undeca-2E-ene-8,10-diynoic acid isobutylamide suppressed production of TNF-α and PGE₂ from infected cells. Dodeca-2E,4E-dienoic acid isobutylamide was especially effective at inhibiting production of these mediators and also strongly inhibited production of G-CSF, CCL2/MCP-1, CCL3/MIP-1α and CCL5/RANTES. In contrast, the ethanol extracts (75%), which were prepared from dormant roots of E. purpurea grown in different locations throughout North Carolina, displayed a range of effects from suppression to stimulation of mediator production. Precipitation of the extracts with ethanol removed the stimulatory activity, however, even after precipitation; many of the extracts did not display any suppressive activity. Analysis of the extracts revealed slight variations in concentration of alkylamides, caftaric acid, and cichoric acid, but the activity of the extracts did not strongly correlate with concentrations of these compounds. Our in vitro experiments suggest that E. purpurea extracts have the potential for use in alleviating the symptoms and pathology associated with infections with influenza A; however, further study will be necessary to define procedures necessary to unmask the alkylamide activity in crude extracts.

Inhibition of H1N1 influenza A virus growth and induction of inflammatory mediators by the isoquinoline alkaloid berberine and extracts of goldenseal (Hydrastis canadensis)

In this study we tested whether the isoquinoline alkaloid berberine can inhibit the growth of influenza A. Our experiments showed strong inhibition of the growth of H1N1 influenza A strains PR/8/34 or WS/33 in RAW 264.7 macrophage-like cells, A549 human lung epithelial-derived cells and murine bone marrow derived macrophages, but not MDCK canine kidney cells. Studies of the mechanism underlying this effect suggest that berberine acts post-translationally to inhibit virus protein trafficking/maturation which in turn inhibits virus growth. Berberine was also evaluated for its ability to inhibit production of TNF-α and PGE(2) from A/PR/8/34 infected-RAW 264.7 cells. Our studies revealed strong inhibition of production of both mediators and suggest that this effect is distinct from the anti-viral effect. Finally, we asked whether berberine-containing ethanol extracts of goldenseal also inhibit the growth of influenza A and production of inflammatory mediators. We found strong effectiveness at high concentrations, although upon dilution extracts were somewhat less effective than purified berberine. Taken together, our results suggest that berberine may indeed be useful for the treatment of infections with influenza A.

Evaluation of a photochemical grid model using estimates of concentration probability density functions

A method for evaluating photochemical grid models based on the estimation of the probability density function (PDF) of the variations in ozone concentrations is described and demonstrated. It is assumed that the ozone concentration PDF is determined from 100 Monte Carlo uncertainty runs based on uncertainties in photochemical grid model input variables. The exercise involves the Urban Airshed Model with Variable grid (UAM-V) as applied to the July 1995 Ozone Transport Assessment Group episode in the eastern US. The focus is on the distribution of model residuals (observed concentration minus model-predicted ensemble mean concentration) for 66 ozone monitors on the OTAG domain. It is concluded that the distribution of observed model residuals is within the 95% range of expected uncertainty, suggesting that the model is performing adequately.

A comparison of regional oxidant model (ROM) output with observed ozone data

Abstract The output from the regional oxidant model (ROM) is compared to observed ozone over northern Illinois for June, July and August 1987. The 8-h daily average ozone at the ozone monitoring stations is interpolated to the ROM grid cells using a spatial statistical method. Differences between the model output and spatial predictions are compared at three levels of spatial averaging (with approximate scales of 19, 100, 400 km) and three levels of temporal averaging (daily, weekly, 3 months). In addition two ozone monitoring stations are paired with weather stations and with ROM cells in order to investigate the performance of ROM as a function of meteorological conditions. For daily values the root mean squared error (RMSE) between the ROM values and those predicted from the monitoring network varies between 14 and 25 ppb with the largest discrepancies occurring near Lake Michigan. Weekly averages reduce the RMSE by approximately 30% but spatial aggregation is not helpful in improving the agreement. The difference between ROM ozone predictions and the observed ozone at two paired sites depends most strongly on temperature and to a lesser extent on dew point temperature. The R 2 from linear regressions is approximately 35%. An examination of the synoptic-scale and meso-scale weather patterns during this period indicates that ROM is sensitive to dynamic situations such as a frontal passage.

Influence of Cover Crops and Inorganic Nitrogen Fertilization on Tomato and Snap Bean Production and Soil Nitrate Distribution

ABSTRACT Commercial vegetable production depends almost solely on inorganic fertilizers. In an era of environmental sensitivity, enrichment of soils with green manures and cover crops may reduce the dependence on these fertilizers while maintaining and enhancing crop yields. The objectives of this research were to determine (1) if supplemental nitrogen (N) at 60 or 120 kg · ha−1 following winter cover crops of wheat (Triticum aestivum L.) or crimson clover (Trifolium incarnatum L.) affect yield of tomato (Lycopersicon esculentum Mill.) and snap bean (Phaseolus vulgaris L.) grown in rotation; and (2) the distribution and retention of soil nitrates in the soil profile as affected by N fertilization and cover cropping. ‘Mountain Pride’ fresh market tomato and ‘Strike’ snap bean were grown in rotation for four years. Clover and wheat cover crops increased total marketable yield of ‘Mountain Pride’ tomato in one out of four years. Total marketable tomato yield increased as fertilizer N increased to 60 kg · ha−1 in two out of four years and with 120 kg · ha−1 in one out of four years. N did not interact with cover crops to affect yield. ‘Strike’ snap bean marketable yield summed over all years was 60% lower in clover plots compared with fallow. Total marketable snap bean yield increased with 60 kg N · ha−1 in one out of three years but was unresponsive to N in two out of three years. Soil nitrates to 1.2 m depth were higher after clover and wheat than after fallow. Nitrate level was highest in soil with clover and 120 kg N · ha−1. In all cover crop or fallow plots, as fertilizer N application levels increased, the soil nitrates also increased. Cover crops or fertilizer N application did not increase the retention of residual nitrates in the 1.2 m soil profile depth after four years of cropping.