Jefferson Rodrigues Gandra

Productive performance and composition of milk protein fraction in dairy cows supplemented with fat sources.

The objective of this study was to evaluate the use of fat sources in rations for lactating cows on the productive performance and composition of milk protein fraction. Twelve Holstein cows were used, grouped in three balanced 4 × 4 Latin squares, fed with the following rations: control; refined soybean oil; whole raw soybean; and calcium salts of unsaturated fatty acid (Megalac-E). Dry matter and nutrient intake, and daily milk production were evaluated. The samples used to analyze milk composition were collected in two alternate days and were obtained from two daily milking. Milk composition and total nitrogen, non-protein nitrogen and non-casein nitrogen ratios were analyzed. The casein, serum protein and true protein ratios were obtained by difference. Dry matter and nutrient intakes were lower when cows received the diet containing calcium salts of fatty acids, in relation to the control diet. Among the diets with fat sources, the one with whole raw soybean and calcium salts decreased milk production. There was no effect of fat sources added to the diet on crude protein, non-protein nitrogen, non-casein nitrogen, true protein, casein, casein/milk true protein ratio and serum protein. Similarly, the experimental diets did not influence the protein fractions when expressed in percentage of milk crude protein. The utilization of fat sources in diets changes milk production and composition of lactating cows, but does not influence the composition of milk protein fractions.

Desempenho e parâmetros sanguíneos de vacas leiteiras alimentadas com grão de soja

The editorial process of Archivos de Zootecnia during 2012 is reported below. A total of 361 manuscripts coming from 28 countries, mainly from Brazil (78 %), were received. Consequently, the language most frequently used in the manuscripts was Portuguese, followed by Spanish and English. The mean time elapsed from submission of a manuscript until printing, was 599 days, a hundred days shorter than 2011. The rejection rate was 65 %. In 2011, 53 articles, 11 short notes and 7 reviews (total 72) were published by authors coming from 14 different countries.

Effects of whole flaxseed, raw soybeans, and calcium salts of fatty acids on measures of cellular immune function of transition dairy cows

The objective of the current study was to evaluate the effects of supplemental n-3 and n-6 fatty acid (FA) sources on cellular immune function of transition dairy cows. Animals were randomly assigned to receive 1 of 4 diets: control (n=11); whole flaxseed (n-3 FA source; n=11), 60 and 80g/kg of whole flaxseed [diet dry matter (DM) basis] during pre- and postpartum, respectively; whole raw soybeans (n-6 FA source; n=10), 120 and 160g/kg of whole raw soybeans (diet DM basis) during pre- and postpartum, respectively; and calcium salts of unsaturated FA (Megalac-E, n-6 FA source; n=10), 24 and 32g/kg of calcium salts of unsaturated FA (diet DM basis) during pre- and postpartum, respectively. Supplemental FA did not alter DM intake and milk yield but increased energy balance during the postpartum period. Diets containing n-3 and n-6 FA sources increased phagocytosis capacity of leukocytes and monocytes and phagocytosis activity of monocytes. Furthermore, n-3 FA source increased phagocytic capacity of leukocytes and neutrophils and increased phagocytic activity in monocytes and neutrophils when compared with n-6 FA sources. Supplemental FA effects on adaptive immune system included increased percentage of T-helper cells, T-cytotoxic cells, cells that expressed IL-2 receptors, and CD62 adhesion molecules. The results of this study suggest that unsaturated FA can modulate innate and adaptive cellular immunity and trigger a proinflammatory response. The n-3 FA seems to have a greater effect on phagocytic capacity and activity of leukocytes when compared with n-6 FA.

Productive performance and milk protein fraction composition of dairy cows supplemented with sodium monensin.

The objective of this work was to evaluate the levels of sodium monensin on lactating cows and their effects on productive performance and milk protein fraction composition. It was used 12 Holstein cows, distributed in four balanced 3 × 3 Latin squares, and fed three diets: one control without monensin, and two diets with monensin at the levels of 24 or 48 mg/kg DM added to the concentrate. Milk production was daily measured throughout the entire experimental period. The samples used for analysis of milk composition were collected on two alternated days from the two daily milking. Non-protein nitrogen, total nitrogen and non-casein nitrogen contents were directly evaluated in the milk, and casein, whey protein and true protein contents were indirectly determined. The use of monensin in the rations reduced dry matter and nutrient intake, especially when diet with 48 mg/kg of dry matter was given. The ration with 24 mg/kg of DM increased milk production, with or without correction, and also fat and lactose yield, and it improved productive efficiency. The levels of monensin in the ratios did not influence contents of milk crude protein, non-protein nitrogen, non-casein nitrogen, true protein, casein, casein/true protein ratio, whey protein, and of all those fractions expressed as percentage of crude protein. The utilization of monensin in the ratio at the dose of 24 mg/kg of DM influences positively the productive performance of lactating cows, and it does not influence the composition of milk protein fractions.

Parâmetros sanguíneos de vacas leiteiras suplementadas com diferentes fontes de gordura

This study was carried out to evaluate the use of different fat sources in dairy cows rations and its effects on dry matter intake, milk yield and composition, and blood parameter. Twelve Holstein cows were allocated in three balanced latin square 4x4, and fed with the following rations: 1) Control, with 2.5%; 2) Refined soybean oil; 3) Whole soybean; and 4), Calcium salts of fatty acids (Megalac-E). The concentrations of total cholesterol and lipoprotein low density (LDL) were higher (P<0.05) for cows that received rations with fat sources, which also causes increased (P<0.05) e concentration of high density lipoprotein (HDL). The concentrations of urea and blood urea nitrogen were similar among the rations, except for the diet containing calcium salts of fatty acids, which showed lower (P<0.05). The dry matter intake was lower (P<0.05) in cows that received calcium salts in the rations. The animals that received a diet containing whole soybeans as a source of fat had lower (P<0.05) yield milk when compared to other rations used. The use of fat sources in dairy cows rations influenced the blood parameters, especially for parameters related to lipidogram.

Fatty acid profile and composition of milk protein fraction in dairy cows fed long-chain unsaturated fatty acids during the transition period

The objective of this study was to evaluate the utilization of different sources of unsaturated long-chain fatty acids in diets for dairy cows during the transition period and early lactation on the milk fatty acid profile and composition of the protein fraction. Thirty-six Holstein cows were divided into three groups, fed the following diets: control (C); soybean oil (SO); and calcium salts of long-chain unsaturated fatty acids (CS). The milk samples utilized for analysis were obtained weekly from parturition to twelve weeks of lactation; each one of the samples originated from two daily milkings. Milk composition and total nitrogen, non-protein nitrogen and non-casein nitrogen levels were analyzed. The cows receiving the diet with calcium salts had lower concentrations of non-protein nitrogen (%CP) in milk compared with the animals fed the diet with soybean oil. There was a decrease in concentration of medium-chain fatty acids C12-C16, and a concomitant increase in concentrations of long-chain fatty acids >C18 in milk fat for the animals fed the diets CS and SO when compared with diet C. Soybean oil and CS diets increased milk-fat concentrations of the acids C18: 1 trans-11, C18: 2 cis-9, trans-11 and C18: 2 trans-10 cis-12 in relation to diet C. The utilization of sources of long-chain fatty acids in the diet of dairy cows increases the biological value of milk in early lactation due to higher concentrations of specific fatty acids such as CLA C18: 2cis-9, trans-11.

Effects of whole raw soybean or whole cottonseed on milk yield and composition, digestibility, ruminal fermentation and blood metabolites of lactating dairy cows

The aim of the present study was to evaluate effects of partial replacement of corn grain and soybean meal with whole raw soybean or whole cottonseed on milk yield and composition, digestibility, ruminal fermentation and blood metabolites. Eighteen multiparous Holstein cows (133.0 ± 53.1 days in milk, 585.2 ± 54.2 kg of BW and 32.22 ± 5.32 kg/day of milk) were used in six 3 × 3 Latin squares with 21-day periods. The cows were randomly assigned to receive one of the following diets: control (CT), diet without oilseed inclusion; whole soybean (WS), inclusion of 120 g/kg of whole raw soybean (diet DM basis); and whole cottonseed (WCS), inclusion of 120 g/kg of whole cottonseed (diet DM basis). Dietary supplementation with WS or WCS had no effect on milk yield and composition. DM and non-fibre carbohydrate intake were decreased in cows fed WS and WCS when compared with CT; however, WS and WCS increased ether extract intake and digestibility. The WCS decreased DM, crude protein, ether extract and non-fibre carbohydrate intake when compared with WS. Cows fed WCS had higher ruminal pH than those fed WS or CT. Cows fed WS showed higher propionate concentration than cows fed WCS. Ruminal ammonia nitrogen concentration decreased when cows were fed WS compared with those fed CT or WCS. The partial replacement of corn grain and soybean meal with WS or WCS in diets of mid-lactating dairy cows decreased DM intake and altered ruminal fermentation, but did not affect milk yield and composition.

Effect of unsaturated fatty acid supplementation on digestion, metabolism and nutrient balance in dairy cows during the transition period and early lactation

The objective of this study was to evaluate the influence of unsaturated fatty acids in diets for dairy cows during the transition period and early lactation on intake, digestion and nutrient balance. Thirty-six multiparous and pregnant Holstein cows were randomly distributed to receive one of the experimental diets in the period from 35 days before the expected date of parturition to 84 days post-partum. Diets were fed as a total mixed ration and were as follows: control (C); soybean oil (SO), based on inclusion of 30 g/kg (DM basis); and calcium salts of unsaturated fatty acids (CS), based on inclusion of 30 g/kg (DM basis). Pre-partum dry matter intakes (DMI) of cows fed C, SO and CS were 11.9, 9.5 and 9.6 kg/d, respectively. Post-partum DMI was affected by experimental diets (18.5, 15.0 and 17.4 kg/d for C, SO and CS, respectively). The energy balance in the transition period of animals fed CS was 4.41 Mcal/d higher than cows fed SO and 1.3 Mcal/d higher than cows fed C. Supplementing cows with unsaturated fatty acid sources is a strategy for dairy cows in the transition period.

Evaluation of the Milk Fatty Acid Profile from Mediterranean Buffalo Cows in the First Eight Weeks of Lactation

The aim of this study was to evaluate the fatty acid composition of buffalo milk in the first eight weeks oflactation. It was used 18 cows multiparous Buffaloes of Mediterranean race, the data collection starting four weeksbefore of the calving provided by the 8th week of lactation. The animals were mechanically milked once daily in themorning. The milk samples used for composition analysis were collected weekly from parturition to eight weeks oflactation. There was effect of weeks during the transition period and early lactation for fat yield (kg/day) and for bodyweight of the buffalo cows in lactation (kg).There was effect of weeks of lactation on the fatty acid composition of milkfat.Variations in levels of unsaturated fatty acids of milk fat of buffaloes are similar to those found in dairy cows of thepartum to eighth week of lactation.

Inoculation of Lactobacillus buchneri alone or with Bacillus subtilis and total losses, aerobic stability, and microbiological quality of sunflower silage

ABSTRACT The purpose of this study was to evaluate the effects of inoculation of Lactobacillus buchneri (L. buchneri) alone or with Bacillus subtilis (B. subtilis) on gas and effluent losses, chemical composition, in vitro digestibility of nutrients, aerobic stability, and microbiological quality of sunflower silage. A randomized experimental design was used, which contained 3 treatments, each one included 15 mini-silos. Mini-silos were distributed to the following treatments: (1) Control (CON), without inoculants; (2) L. buchneri alone (LB), inoculation at 2.6 × 1010 cfu/g; and (3) L. buchneri with B. subtilis (LB + BS), inoculation at 2.6 × 1010 cfu/g and 1 × 109 cfu/g with L. buchneri and B. subtilis, respectively. Treatments were applied at 2 g/t of fresh forage ensiled. Silages with microbial inoculants had lower DM content, and higher in vitro digestibility of DM and neutral detergent fibre than CON. Inoculants decreased the number of aerobic bacteria, mould, and yeast, and increased the counts of lactic acid bacteria in sunflower silage. L. buchneri exhibited positive effects on aerobic stability, in vitro digestibility of nutrients and decreased the counts of mould and yeast, but did not show a synergistic effect with B. subtilis on sunflower silage.