Jeffrey R. Fishpaugh

Immunoassay reagents for psychoactive drugs. Part 4. Quantitative determination of amitriptyline and nortriptyline by fluorescence polarization immunoassay.

Methods for the quantitative determination of amitriptyline and nortriptyline by fluorescence polarization immunoassay (FPIA) is described. One immunoassay allows for the accurate quantification of amitriptyline in the presence of nortriptyline while the second immunoassay allows for the accurate quantification of nortriptyline in the presence of amitriptyline.

Preparation and high-performance liquid chromatographic analysis of syn and anti isomers of steroidal 3-(O-carboxymethyl) oximes

Abstract Syn and anti isomeric mixtures of methyl esters of 3-(O-carboxymethyl) oximes of progesterone, 17-α-OH-progesterone, testosterone, and cortisol were synthesized and separated on a preparative scale. The pure esters were then hydrolysed to afford the desired syn or anti 3-(O-carboxymethyl) oximes. Analysis by HPLC was developed to determine isomeric and chemical purity of the acids and esters.

Lipase mediated diastereoselective hydrolysis of steroidal 3-(O-carboxymethyl) oxime methyl esters

Abstract A series of geometric isomeric mixtures of steroidal 3-(O-Carboxymethyl) oxime methyl esters 1–4 were hydrolyzed with high diastereoselectivity for the anti isomer with crude lipase from Candida cylindracea .

Immunoassay reagents for psychoactive drugs. Part 5. Quantitative determination of imipramine and desipramine by fluorescence polarization immunoassay.

Two methods for the quantitative determination of imipramine (IMI) and desipramine (DMI) by fluorescence polarization immunoassay (FPIA) are described. One immunoassay allows for the accurate quantification of imipramine in the presence of desipramine, while the other allows for the accurate quantification of desipramine in the presence of imipramine.