Jelena Selanec
University of Zagreb
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BMC Veterinary Research | 2014
Josipa Kuleš; Vladimir Mrljak; Renata Barić Rafaj; Jelena Selanec; Richard Burchmore; P.D. Eckersall
BackgroundCanine babesiosis is a tick-borne disease that is caused by the haemoprotozoan parasites of the genus Babesia. There are limited data on serum proteomics in dogs, and none of the effect of babesiosis on the serum proteome. The aim of this study was to identify the potential serum biomarkers of babesiosis using proteomic techniques in order to increase our understanding about disease pathogenesis.ResultsSerum samples were collected from 25 dogs of various breeds and sex with naturally occurring babesiosis caused by B. canis canis. Blood was collected on the day of admission (day 0), and subsequently on the 1st and 6th day of treatment.Two-dimensional electrophoresis (2DE) of pooled serum samples of dogs with naturally occurring babesiosis (day 0, day 1 and day 6) and healthy dogs were run in triplicate. 2DE image analysis showed 64 differentially expressed spots with p ≤ 0.05 and 49 spots with fold change ≥2. Six selected spots were excised manually and subjected to trypsin digest prior to identification by electrospray ionisation mass spectrometry on an Amazon ion trap tandem mass spectrometry (MS/MS). Mass spectrometry data was processed using Data Analysis software and the automated Matrix Science Mascot Daemon server. Protein identifications were assigned using the Mascot search engine to interrogate protein sequences in the NCBI Genbank database.A number of differentially expressed serum proteins involved in inflammation mediated acute phase response, complement and coagulation cascades, apolipoproteins and vitamin D metabolism pathway were identified in dogs with babesiosis.ConclusionsOur findings confirmed two dominant pathogenic mechanisms of babesiosis, haemolysis and acute phase response. These results may provide possible serum biomarker candidates for clinical monitoring of babesiosis and this study could serve as the basis for further proteomic investigations in canine babesiosis.
Journal of Veterinary Internal Medicine | 2013
R. Barić Rafaj; Josipa Kuleš; Jelena Selanec; N. Vrkić; V. Zovko; M. Zupančič; A. Trampuš Bakija; Vesna Matijatko; Martina Crnogaj; Vladimir Mrljak
BACKGROUND Babesia infections in dogs can result in a wide range of clinical and laboratory presentations, including coagulopathy. Expression of intercellular adhesion molecule-1 (ICAM-1) and von Willebrand factor (vWF) in dogs with babesiosis is unknown. OBJECTIVES Whether inflammation in babesiosis triggers activation of ICAM-1 and the coagulation system. ANIMALS Twelve and 10 dogs with naturally occurring babesiosis before and after antiparasitic treatment, respectively, were compared with 10 healthy dogs. METHODS In this prospective study, diagnosis was made by blood smear examination and confirmed by PCR. C-reactive protein (CRP), soluble intercellular adhesion molecule 1 (sICAM-1), and von Willebrand factor (vWF) levels were measured by a canine ELISA kit, fibrinogen (FIB) and factor VIII activity levels were measured by coagulometric methods, and blood cell counts (WBC, RBC, PLT) were determined with an automatic analyzer. RESULTS Compared to healthy dogs, the CRP, sICAM-1, and FIB concentrations were significantly increased before therapy and remained high for 3 days after therapy in dogs with babesiosis. vWF activity was significantly decreased in dogs with babesiosis before treatment. FVIII activity did not differ between dogs with babesiosis and healthy dogs. WBC; RBC and PLT were significantly lower before treatment and normalized by 3 days after treatment. CONCLUSION AND CLINICAL IMPORTANCE A proinflammatory condition in babesiosis appears to influence endothelial dysfunction and hemostatic activity. Although clearly beneficial for the parasite, sequestered blood cells can obstruct blood flow in small vessels, promote an inflammatory state, and could increase the severity of babesiosis.
Farm animal proteomics 2013 | 2013
Josipa Kuleš; Vladimir Mrljak; Renata Barić Rafaj; Jelena Selanec; Richard Burchmore; P.D. Eckersall
Canine babesiosis is a tick-borne disease that is caused by the haemoprotozoan parasites of the genus Babesia (Taboada and Merchant, 1991). There are limited data of serum proteomics in dogs, and none of babesiosis. The aim of this study was to identify the potential serum biomarkers using proteomic techniques and to increase our understanding about disease pathogenesis.
Veterinarski Arhiv | 2012
Nika Brkljača Bottegaro; Josip Kos; Ozren Smolec; Dražen Vnuk; Dražen Matičić; Boris Pirkić; Berislav Radišić; Zoran Vrbanac; Jelena Selanec
Veterinarski Arhiv | 2015
Martina Crnogaj; Ivana Kiš; Nada Kučer; Iva Šmit; Iva Mayer; Mirna Brkljačić; Jelena Selanec; Vladimir Mrljak
Veterinarski Arhiv | 2015
Damjan Gračner; Ljubo Barbić; Inga Bijader; Petra Čolig; Gordana Gregurić Gračner; Jelena Selanec; Robert Zobel; Vladimir Stevanović; Marko Samardžija
Zbornik radova Veterinarski dani 2014 | 2014
Darko Capak; Berislav Radišić; Jelena Selanec; Marko Pećin; Valentina Gusak; Melita Majhut; Nika Brkljača Bottegaro
Zbornik radova Veterinarski dani 2014 | 2014
Darko Capak; Hrvoje Capak; Berislav Radišić; Jelena Selanec; Ivica Harapin; Ana Bruić; Nika Brkljača Bottegaro
XX SIVE international congress Milano, 7 th -9 th February 2014 | 2014
Jelena Selanec; Nika Brkljača Bottegaro; Boris Pirkić; Vladimir Mrljak
XX SIVE international congress Milano, 7 th -9 th February 2014 | 2014
Nika Brkljača Bottegaro; Jelena Selanec; Eleonora Bačani; Ozren Smolec; Darko Grden; Josip Kos