Jen-Min Kuo
National Kaohsiung Marine University
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Featured researches published by Jen-Min Kuo.
Molecular Nutrition & Food Research | 2008
Min-Hsiung Pan; Min-Chi Hsieh; Jen-Min Kuo; Ching-Shu Lai; Hou Wu; Shengmin Sang; Chi-Tang Ho
Ginger, the rhizome of Zingiber officinale, is a traditional medicine with anti-inflammatory and anticarcinogenic properties. This study examined the growth inhibitory effects of the structurally related compounds 6-gingerol and 6-shogaol on human cancer cells. 6-Shogaol [1-(4-hydroxy-3-methoxyphenyl)-4-decen-3-one] inhibits the growth of human cancer cells and induces apoptosis in COLO 205 cells through modulation of mitochondrial functions regulated by reactive oxygen species (ROS). ROS generation occurs in the early stages of 6-shogaol-induced apoptosis, preceding cytochrome c release, caspase activation, and DNA fragmentation. Up-regulation of Bax, Fas, and FasL, as well as down-regulation of Bcl-2 and Bcl-X(L )were observed in 6-shogaol-treated COLO 205 cells. N-acetylcysteine (NAC), but not by other antioxidants, suppress 6-shogaol-induced apoptosis. The growth arrest and DNA damage (GADD)-inducible transcription factor 153 (GADD153) mRNA and protein is markedly induced in a time- and concentration-dependent manner in response to 6-shogaol.
Journal of Agricultural and Food Chemistry | 2010
Min-Hsiung Pan; Mei-Ling Tsai; Wen-Ming Chen; Ann Hwang; Bonnie Sun Pan; Yu-Ren Hwang; Jen-Min Kuo
The objective of the present study is to purify and characterize the fish scale-degrading enzyme from Vogesella sp.7307-1, which was newly identified and isolated from fish scales. The enzyme from Vogesella sp.7307-1 was assayed with casein and confirmed as a protease. Crude protease was extracted, isolated, and purified 35.7-fold with 19.6% recovery using 20-80% saturation of ammonium sulfate fractionation, Q FF ion exchange chromatography, and Superdex 200 gelfiltration. The molecular weight of the purified enzyme was 119 kDa. The Km and Vmax were 0.067 mM and 425.5 U/mg-min, respectively using azo-casein as substrate. The optimum pH of the purified enzyme was 7.5, and the optimum temperature was 50 °C. The enzyme was stable at temperatures below 55 °C and pH range 7.5 to 9.0. The enzyme activity of the purified protease was completely inhibited by EDTA (ethylene diamine teraacetates), indicating the enzyme was a metalloprotease. Hydrolysates from fish scales treated with protease 7307-1 were found having low molecular weight peptides (<1 kDa). The protease 7307-1 is a promising enzyme for preparing smaller peptides from fish scales.
International Journal of Food Engineering | 2011
Jing-Iong Yang; Jen-Min Kuo; Wen-Ming Chen; Huei-Jing Ke; Yi-Ju Chou
After undergoing keratinase digestion, feather wastes could have a great potential as a source of proteins and amino acids for many applications. In this study, the fermentation conditions of feather-degrading Meiothermus sp. strain I40 (I40) were optimized to enhance the biodegradation of chicken feather to hydrolysates. Initially, the factors essential for I40 keratinase production in submerged fermentation were screened, whereas response surface methodology (RSM) was then employed to evaluate the interactions among the effective factors. At first stage, eight fermentation parameters were screened using a Plackett-Burman (PB) design. Four effective factors identified by PB screening, namely feather concentration, tryptone concentration, yeast extract concentration, and incubation temperature, were further investigated their effects on keratinase production by RSM using central composite design (CCD). The I40 fermentation conditions for maximal keratinase activity were as follows: tryptone concentration 0.16 percent (w/v), yeast extract concentration 0.27 percent (w/v), feather concentration 0.08 percent (w/v), and incubation temperature at 51.7°C for 72 hr under 120 rev/min shaking. Compared to the initial stage, a 13.3-fold increase in keratinase activity was achieved when I40 incubated in the optimized conditions.
Journal of Agricultural and Food Chemistry | 1999
Jen-Min Kuo; Dong-Bor Yeh; Bonnie Sun Pan
Journal of Agricultural and Food Chemistry | 2006
Jen-Min Kuo; Ann Hwang; Dong-Bor Yeh; Min-Hsiung Pan; Mei-Ling Tsai; Bonnie Sun Pan
Journal of Agricultural and Food Chemistry | 1996
Jen-Min Kuo; Ann Hwang; and Hsiu Hua Hsu; Bonnie Sun Pan
Journal of Agricultural and Food Chemistry | 1997
Jen-Min Kuo; Ann Hwang; Dong-Bor Yeh
Journal of Food Biochemistry | 1986
Bonnie Sun Pan; Jen-Min Kuo; Lih-Ju Luo; Hei-Min Yang
International Biodeterioration & Biodegradation | 2012
Jen-Min Kuo; Jing-Iong Yang; Wen-Ming Chen; Min-Hsiung Pan; Mei-Ling Tsai; Ying-Jang Lai; Ann Hwang; Bonnie Sun Pan; Chueh-Yueh Lin
Journal of Agricultural and Food Chemistry | 2006
An-Chin Cheng; Tzou-Chi Huang; Ching-Shu Lai; Jen-Min Kuo; Ying-Tang Huang; Chih-Yu Lo; Chi-Tang Ho; Min-Hsiung Pan