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Dive into the research topics where Jenn-Haung Lai is active.

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Featured researches published by Jenn-Haung Lai.


Journal of Immunology | 2001

Infection of Human Dendritic Cells by Dengue Virus Causes Cell Maturation and Cytokine Production

Ling‐Jun Ho; Jaang-Jiun Wang; Men-Fang Shaio; Chuan-Liang Kao; Deh-Ming Chang; Shou-Wha Han; Jenn-Haung Lai

Dengue virus (DV) infection is a major problem in public health. It can cause fatal diseases such as Dengue hemorrhagic fever and Dengue shock syndrome. Dendritic cells (DC) are professional APCs required for establishing a primary immune response. Here, we investigated the role of human PBMC-derived DC in DV infection. Using different techniques, including plaque assay, flow cytometry analysis, nested RT-PCR, and confocal microscope and electron microscope examinations, we show that DV can enter cultured human DC and produce virus particles. After entrance, DV could be visualized in cystic vesicles, vacuoles, and the endoplasmic reticulum. The DV-infected DC also showed proliferation and hypertrophy of the endoplasmic reticulum as well as the swollen mitochondria. In addition, the DV-stimulated DC could express maturation markers such as B7-1, B7-2, HLA-DR, CD11b, and CD83. Furthermore, the infection of DC by DV induced production of TNF-α and IFN-α, but not IL-6 and IL-12. Although DC underwent spontaneous apoptosis in the absence of feeding cytokines, this process appeared to be delayed after DV infection. Our observations provide important information in understanding the pathogenesis of DV infection.


Biochemical Pharmacology | 2003

Down-regulation of c-jun N-terminal kinase-activator protein-1 signaling pathway by Ginkgo biloba extract in human peripheral blood T cells.

Shu-Meng Cheng; Shih-Ping Yang; Ling-Jun Ho; Tien-Ping Tsao; Ting-Yi Juan; Deh-Ming Chang; Sun-Yran Chang; Jenn-Haung Lai

The activation of T lymphocytes contributes to inflammatory process of cardiovascular and cerebrovascular diseases. We investigated the effects of the extract of Ginkgo biloba (EGb), an ancient plant preserving antioxidant property, on phorbol 12-myristate 13-acetate+ionomycin or anti-CD3+anti-CD28 monoclonal antibodies-activated T cells. Human peripheral blood T cells were negatively selected from whole blood. Cytokines were measured by ELISA, cell surface markers by flow cytometry and the activities of transcription factors and kinases were determined by electrophoresis mobility shift assays, kinase assays and transfection assays. We showed that EGb inhibited several cytokines, including tumor necrosis factor-alpha, interleukin (IL)-2, IL-4 and interferon-gamma production from activated T cells. Electrophoresis mobility shift assay analysis indicated that EGb down-regulated activator protein-1 (AP-1) but not nuclear factor kappa B DNA-binding activity. In addition, EGb inhibited c-jun N-terminal kinase but not extracellular signal regulated protein kinase activity. The inhibitory specificity on AP-1 by EGb was also demonstrated in transfection assays. The inhibition of AP-1 signaling pathway in T cells by EGb provides a support for its efficacy in cardiovascular and cerebrovascular diseases and raises a therapeutic potential for this drug in activated T cell-mediated pathologies.


Rheumatology International | 2007

Autoimmune hepatitis with raised alpha-fetoprotein level as the presenting symptoms of systemic lupus erythematosus: a case report

Feng-Cheng Liu; Deh-Ming Chang; Jenn-Haung Lai; Chih-Kung Lin; Hsiang-Cheng Chen; Tsung-Yun Hou; San-Yuan Kuo

Systemic lupus erythematosus (SLE) and autoimmune hepatitis are distinct clinical disorders, which rarely occur, in the same patient. We describe a 59-year-old woman with coexistence of both conditions. Photosensitivity, arthritis, positive ANA, and extreme elevation of anti-dsDNA concluded the diagnosis of SLE. Hyperbilirubinemia, high serum value of liver function, and elevation of alpha-fetoprotein were also prominent. By a review of pertinent literature, clinical investigation, calculation of autoimmune hepatitis score, and pathology of liver biopsy specimen, we were in favor of autoimmune hepatitis. Awareness of this rare presentation may be beneficial to clinicians in identifying and treating patients with both SLE and autoimmune hepatitis.


Immunological Investigations | 2003

Carvedilol Modulates In‐Vitro Granulocyte‐Macrophage Colony‐Stimulating Factor‐Induced Interleukin‐10 Production in U937 Cells and Human Monocytes

Shu-Meng Cheng; Shih-Ping Yang; Ling‐Jun Ho; Tien‐Ping Tsao; Deh-Ming Chang; Jenn-Haung Lai

Both granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) and interleukin‐10 (IL‐10) are important mediators regulating inflammatory responses. Inflammatory processes have an important role in atherogenesis. In this paper, the effects of carvedilol on GM‐CSF‐induced IL‐10 production were examined on human monocytic cell line, U937, and purified human monocytes. First, we showed that one‐time carvedilol pretreatment at concentrations 0.3–10 μM dose‐dependently inhibited GM‐CSF‐induced IL‐10 production in U937 cells. In addition, we found carvedilol to be non‐cytotoxic at concentrations equal to or less than 10 μM. However, at concentrations higher than 10 μM, carvedilol induced programmed cell death in U937 cells. The inhibition of GM‐CSF‐induced IL‐10 production by carvedilol was also observed at the expression of mRNA. Furthermore, the inhibition of IL‐10 production was demonstrated in GM‐CSF‐activated purified human peripheral blood monocytes. Finally, long‐term carvedilol pretreatment of U937 cells up to 2 months at concentrations of 1.0 μM mildly enhanced the IL‐10 production. Our observations that carvedilol modulated GM‐CSF‐induced IL‐10 production may have some implication in understanding the broad‐spectrum effects of carvedilol in regulating inflammatory reactions.


Formosan Journal of Rheumatology | 2009

Evaluation of Calcium Pyrophosphate Dihydrate Deposition Disease by Ultrasound

Kang-Min Lin; Jenn-Haung Lai; Deh-Ming Chang; San-Yuan Kuo; Chen-Hung Chen; Tsung-Yun Hou; Feng-Cheng Liu; Chi-Ching Chang

Objective: Our aim was to characterize the ultrasonographic features of patients with calcium pyrophosphate dihydrate (CPPD) deposition disease, and compare X-ray and ultrasound in evaluating CPPD deposition disease. Methods: In this retrospective study, all 71 patients between 2004 and 2007 with CPPD deposition disease proved by microscopic synovial fluid analysis were enrolled. We collected and analyzed 38 patients of those, on whom both conventional X-ray and high-resolution ultrasound had been carried out. Results: All patients were elderly (i.e.>65y/o) and mostly coexisted with osteoarthritis. The involvement of knee joint was the most common site. Popliteal cyst was detected in 9 of 71 patients. Synovial fluid analysis of 38 patients with CPPD deposition disease revealed that the average total white cell count was 25592.1±16697.8/mm^3, with significant neutrophil predominance. There was significant evidence that ultrasound was more reliable than X-ray in the diagnosis of CPPD deposition disease (p=0.002). Besides, there were no patients with CPPD deposition disease in whom X-rays suggested CPPD deposition disease, but for whom ultrasound results were negative. Conclusion: We found that bright stippled foci in the synovial fluid or around the articular region, the thin hyperechoic band parallel to the surface of the hyaline cartilage, and the calcification of fibrocartilage seen on ultrasound could represent CPPD deposits. Our data showed that ultrasound is a useful and important tool in the diagnostic investigation of patients with CPPD deposition disease.


Formosan Journal of Rheumatology | 2006

A Comparison of the Diagnostic Sensitivity and Specificity of Two Anti-cyclic Citrullinated Peptides (CCP1 and CCP2) Tests for Rheumatoid Arthritis

Feng-Cheng Liu; Hsiang-Cheng Chen; Deh-Ming Chang; Chen-Hung Chen; Tsung-Yun Hou; San-Yuan Kuo; Jenn-Haung Lai

The detection of anti-cyclic citrullinated peptide (CCP) autoantibodies has been considered a useful tool to identify rheumatoid arthritis (RA) patients. Here, we examined the sensitivity and specificity of two detection methods, namely the ELISA-based (anti-CCP1) and the EliA-based (anti-CCP2) for anti-CCP in 40 RA patients. Methods. Anti-CCP1 and anti-CCP2 antibody tests were performed on 79 patients with arthropathy. The sensitivity, specificity, positive predictive value, and negative predictive value for-discriminating between rheumatoid arthritis (RA) and non-RA were calculated for both tests. Results. Both anti-CCP1 and anti-CCP2 shared very similar specificities (97.4%) toward diagnosing RA. However, there was no significant associations between the titer of anti-CCP1 and anti-CCP2. Conclusion. Anti-CCP test was more sensitive and specific than traditional IgM-RF. However, anti-CCP2 was not significantly better than anti-CCP1 in the diagnosis of RA.


Formosan Journal of Rheumatology | 2003

Life Threatening Pancytopenia with Azathioprine in a Systemic Lupus Erythematosus Patient

Hsiang-Cheng Chen; Deh-Ming Chang; San-Yuan Kuo; Chen-Hung Chen; Jenn-Haung Lai

Severe and rapidly evolving pancytopenia due to toxicity from low dosage (<3 mg/kg/day) azathioprine (AZA) in patients with autoimmune diseases is uncommon. We describe a 38-year-old woman with systemic lupus erythematosus (SLE) who developed pancytopenia 5 week after low-dose AZA (0.75 mg/kg/day) was started as a corticosteroid – sparing agent. After cessation of AZA, the clinical and hematologic abnormalities resolved. We report here a case of early and severe myelosuppression associated with AZA and review similar cases reported in the literature.


Formosan Journal of Rheumatology | 2012

Plasma Exchange as the Rescuing Therapy for Critical Diffuse Alveolar Hemorrhage in Systemic Lupus Erythematosus: Experiences of 28 Patients in One Center

Sheng-Hong Lin; Chun-Chi Lu; Chen-Hung Chen; Tony Szu Hsien Lee; Jenn-Haung Lai; Shan-Yuan Kuo; Deh-Ming Chang

Objective: To determine the role of plasma exchange (PE) in patients with systemic lupus erythematosus (SLE) with diffuse alveolar hemorrhage (DAH).Methods: We performed a retrospective analysis to evaluate patients in critical condition with SLE with DAH who underwent rescue plasma exchange (RPE) between February 1985 and January 2012.Results: Our study included 28 patients with SLE with DAH. Twenty-two patients received PE with or without pulse methylprednisolone therapy as rescue therapies and other six patients did not undergo the PE. Overall PEs were performed 189 sessions, with a median of seven sessions for each patient per course (range, 2-33). Four of these patients died at therapies: one of septicemia, one of DAH with acute respiratory failure, and two of cardiac thrombosis with circulation failure. The overall 5-year survival rate was better in patients receiving RPE (64% vs. 33%). No patient developed anaphylaxis. Patients received methylprednisolone pulse therapy (1500-6000 mg) simultaneously with or separately from RPE. In all courses, we evaluated the disease activity with the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and the mean SLEDAI scores were 24.9 and 9.1 before and 3 weeks after RPE, respectively.Conclusions: Our data suggest that the patients with SLE in critical condition of DAH not receiving PE may have a higher immediate mortality rate in comparison with those receiving PE with or without concomitant pulse methylprednisolone therapy.


Formosan Journal of Rheumatology | 2009

Predictive Value of erythrocyte-bound C4d to erythrocyte-complement Receptor Type 1 Ratio in Lupus Disease Activity

Deng-Ho Yang; Deh-Ming Chang; Tsung-Yun Hou; Song-Feng Yeh; San-Yuan Kuo; Jenn-Haung Lai; Chen-Hung Chen

Objective: Erythrocyte-bound C4d (E-C4d) and erythrocyte-complement receptor type 1 (E-CR1) are both novel biomarkers to diagnose systemic lupus erythematosus (SLE) and to monitor disease activity. This study aimed at determining the ratio of E-C4d to E-CR1 (E-C4d/E-CR1) using mouse monoclonal antibody CR1-2B11 and correlating the clinical association of this ratio with disease activity of SLE. Methods: This study included 6 SLE patients, and their blood samples were collected before and after immunosuppressive therapy. E-C4d and E-CR1 levels were measured using indirect immunofluorescence and flow cytometry on the same day as the blood drawing, and the E-C4d/E-CR1 ratios were calculated from the values obtained. Results: The mean of the E-C4d/E-CR1 ratio was significantly decreased in SLE patients after immunosuppressive therapy (12.93±1.9 vs. 3.52±0.9, p<0.05). Three of the 6 SLE patients showed decreased ratios when their active disease stabilized after adequate treatment respectively (19.23 to 1.01, 5.47 to 1.25, and 13.16 to 2.40). Conclusion: The reciprocal change in E-C4d and E-CR1 levels is important in SLE patients. The E-C4d/E-CR1 ratio assessed with CR1-2B11 could be a good biomarker to predict disease activity and may be helpful in formulating a therapeutic strategy in SLE.


Formosan Journal of Rheumatology | 2007

The Prevalence of HLA-B27 Subtypes in AS Patients

Jao-An Chen; Tsung-Yun Hou; Feng-Cheng Liu; Chen-Hung Chen; Jenn-Haung Lai; Deh-Ming Chang; San-Yuan Kuo

Background: Ankylosing spondylitis (AS) is an HLA-B27-related spondyloarthropathy (SpA). Most AS patients carry the HLA-B27 genotype, but only a small fraction of HLA-B27 positive people develop AS. This article describes our attempt to predict the probability of AS in Taiwanese SpA patients who have different HLA-B27 subtypes. Methods: We obtained blood specimens from 251 outpatients suffering from HLA-B27-related SpA. There were 197 AS cases and 54 non-AS cases. We used a standard method PCR-SSOP to determine HLA-B27 subtypes. Results: We found 4 HLA-B27 genotypes among our patients. Among the 197 AS patients, 191 had the B*2704 genotype, 4 had the B*2705 genotype, and 2 had the B*2706 genotype. Among the 54 non-AS patients, 47 had the B*2704 genotype, 5 had the B*2705 genotype, 1 had the B*2706 genotype, and 1 had the B*2707 genotype. Among our SpA patients, our results indicate a stronger association of the B*2704 genotype with AS, and a stronger association of the B*2705 genotype with the absence of AS. Conclusion: The results with our population of Taiwanese patients indicate limited usefulness of HLA-B27 subtyping for prediction AS in SpA patients from Taiwan.

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Deh-Ming Chang

National Defense Medical Center

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Chen-Hung Chen

National Defense Medical Center

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San-Yuan Kuo

National Defense Medical Center

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Tsung-Yun Hou

National Defense Medical Center

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Feng-Cheng Liu

National Defense Medical Center

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Hsiang-Cheng Chen

Tri-Service General Hospital

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Chi-Ching Chang

Taipei Medical University Hospital

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Hang-Ching Lin

National Defense Medical Center

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Ling‐Jun Ho

Tri-Service General Hospital

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Shih-Ping Yang

Tri-Service General Hospital

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