Jennifer Moore-Kucera

Degradation of Potentially Biodegradable Plastic Mulch Films at Three Diverse U.S. Locations

For widespread adoption of biodegradable plastics as agricultural mulches, dependable biodegradation across contrasting conditions is necessary. The in situ degradation of four potentially biodegradable mulches (two commercially available starch-based films, one commercially available cellulose paper mulch, and one experimental spunbond polylactic acid mulch) were evaluated by measuring percentage of area remaining (PMAR) after burial for 6, 12, 18, and 24 months in high tunnel and open field tomato production systems at three geographically distinct U.S. locations (Knoxville, TN; Lubbock, TX; Mount Vernon, WA). The PMAR of the mulches did not differ between high tunnel and open field systems at any location, and PMAR of cellulose mulch was 0% within 12 months but >90% for experimental spunbond at 24 months. The PMAR of the two starch-based products did vary by location and was lowest at Lubbock (˜2%) compared to Knoxville (49%) or Mount Vernon (89%). Relative to the other two locations, Lubbock had the greatest soil diurnal temperature range, maximum daily soil temperature, an alkaline soil pH, and a microbial community structure characterized by a relatively high abundance of fungi. Mulch type and geographic location exerted a greater influence on PMAR than did production system, and abiotic and biotic variables influenced degradation.

Isolation of native soil microorganisms with potential for breaking down biodegradable plastic mulch films used in agriculture.

Fungi native to agricultural soils that colonized commercially available biodegradable mulch (BDM) films were isolated and assessed for potential to degrade plastics. Typically, when formulations of plastics are known and a source of the feedstock is available, powdered plastic can be suspended in agar-based media and degradation determined by visualization of clearing zones. However, this approach poorly mimics in situ degradation of BDMs. First, BDMs are not dispersed as small particles throughout the soil matrix. Secondly, BDMs are not sold commercially as pure polymers, but rather as films containing additives (e.g. fillers, plasticizers and dyes) that may affect microbial growth. The procedures described herein were used for isolates acquired from soil-buried mulch films. Fungal isolates acquired from excavated BDMs were tested individually for growth on pieces of new, disinfested BDMs laid atop defined medium containing no carbon source except agar. Isolates that grew on BDMs were further tested in liquid medium where BDMs were the sole added carbon source. After approximately ten weeks, fungal colonization and BDM degradation were assessed by scanning electron microscopy. Isolates were identified via analysis of ribosomal RNA gene sequences. This report describes methods for fungal isolation, but bacteria also were isolated using these methods by substituting media appropriate for bacteria. Our methodology should prove useful for studies investigating breakdown of intact plastic films or products for which plastic feedstocks are either unknown or not available. However our approach does not provide a quantitative method for comparing rates of BDM degradation.

Interactions of Soil Order and Land Use Management on Soil Properties in the Kukart Watershed, Kyrgyzstan

Surveys of soil properties related to soil functioning for many regions of Kyrgyzstan are limited. This study established ranges of chemical (soil organic matter (SOM), pH and total N (TN)), physical (soil texture), and biochemical (six enzyme activities of C, N, P, and S cycling) characteristics for nine profiles from the Kukart watershed of Jalal-Abad region in Kyrgyzstan. These profiles represent different soil orders (Inceptisols, Alfisols, and Mollisols) and land uses (cultivated, nut-fruit forests, and pasture). The Sierozem (Inceptisols) soils had the highest pH and contained the lowest SOM and TN contents compared to the Brown, Black-brown, and Meadow-steppe soils (Alfisols and Mollisols). Enzymatic activities within surface horizons (0–18 cm) typically decreased in the following order: forest > pasture > cultivated. Enzyme activity trends due to land use were present regardless of elevation, climate, and soil types although subtle differences among soil types within land use were observed. The significant reductions in measured soil enzyme activities involved in C, N, P, and S nutrient transformations under cultivation compared to pasture and forest ecosystems and lower values under Inceptisols can serve as soil quality indicators for land use decisions in the watershed.