Jeong-Cheol Lee

Ex vivo bone morphogenetic protein 2 gene delivery using periodontal ligament stem cells for enhanced re-osseointegration in the regenerative treatment of peri-implantitis

Peri-implantitis is a chronic inflammatory process with advanced bone loss and impaired healing potential. For peri-implantitis treatment, tissue engineering can be applied to enhance bone regeneration of peri-implant defects. This study aimed to evaluate ex vivo bone morphogenetic protein 2 (BMP2) gene delivery using canine periodontal ligament stem cells (PDLSCs) for regeneration of peri-implantitis defects. Canine PDLSCs were transduced with adenoviral vectors containing BMP2 (BMP2/PDLSCs). After peri-implantitis was induced by ligature placement in six beagle dogs, regenerative procedures were performed; hydroxyapatite (HA) particles and collagen gel with autologous canine PDLSCs (PDLSC group) or BMP2/PDLSCs (BMP/PDLSC group) or without cells (control group) were grafted into the defects and covered by an absorbable membrane. Three months later, the animals were sacrificed. In vitro, BMP2/PDLSCs showed similar levels of stem cell properties to PDLSCs, such as colony-forming efficiency and expression of MSC markers STRO-1 and CD 146. BMP2/PDLSCs produced BMP-2 until day 21 at a concentration of 4-8 ng/mL. In vivo, the BMP2/PDLSC group showed significantly more new bone formation and re-osseointegration in peri-implantitis defects compared to the other groups. In conclusion, ex vivo BMP2 gene delivery using PDLSCs enhanced new bone formation and re-osseointegration in peri-implantitis defects.

Enhanced osteoconductivity of sodium‐substituted hydroxyapatite by system instability

The effect of substituting sodium for calcium on enhanced osteoconductivity of hydroxyapatite was newly investigated. Sodium-substituted hydroxyapatite was synthesized by reacting calcium hydroxide and phosphoric acid with sodium nitrate followed by sintering. As a control, pure hydroxyapatite was prepared under identical conditions, but without the addition of sodium nitrate. Substitution of calcium with sodium in hydroxyapatite produced the structural vacancies for carbonate ion from phosphate site and hydrogen ion from hydroxide site of hydroxyapatite after sintering. The total system energy of sodium-substituted hydroxyapatite with structural defects calculated by ab initio methods based on quantum mechanics was much higher than that of hydroxyapatite, suggesting that the sodium-substituted hydroxyapatite was energetically less stable compared with hydroxyapatite. Indeed, sodium-substituted hydroxyapatite exhibited higher dissolution behavior of constituent elements of hydroxyapatite in simulated body fluid (SBF) and Tris-buffered deionized water compared with hydroxyapatite, which directly affected low-crystalline hydroxyl-carbonate apatite forming capacity by increasing the degree of apatite supersaturation in SBF. Actually, sodium-substituted hydroxyapatite exhibited markedly improved low-crystalline hydroxyl-carbonate apatite forming capacity in SBF and noticeably higher osteoconductivity 4 weeks after implantation in calvarial defects of New Zealand white rabbits compared with hydroxyapatite. In addition, there were no statistically significant differences between hydroxyapatite and sodium-substituted hydroxyapatite on cytotoxicity as determined by BCA assay. Taken together, these results indicate that sodium-substituted hydroxyapatite with structural defects has promising potential for use as a bone grafting material due to its enhanced osteoconductivity compared with hydroxyapatite.

Developing performance measurement system for Internet of Things and smart factory environment

To cope with large fluctuations in the demand of a commodity, it is necessary for the manufacturing system to have rapid reactive ability. This requirement may be secured by performance measurement. Although manufacturing companies have used information systems to manage performance, there has been the difficulty of capturing real-time data to depict real situations. The recent development and application of the Internet of Things (IoT) has enabled the resolution of this problem. In demonstration of the functionality of IoT, we developed an IoT-based performance model consistent with the ISA-95 and ISO-22400 standards, which define manufacturing processes and performance indicator formulas. The development comprised three steps: (1) Selection of the Key Performance Indicators of the Overall Equipment Effectiveness (OEE), and the development of an IoT-based production performance model, (2) Implementation of the IoT-based architecture and performance measurement process using Business Process Modelling and (3) Validation of the proposed model through virtual factory simulation. We investigated the effect of the IoT-workability on the OEE, based on the final results of the simulation, both for the planned and actual productions. The simulation results showed that the proposed model represented the timestamp data acquired by IoT and captured the entire production process, thus enabling the determination of real-time performance indicators.

Effect of precursor concentration and spray pyrolysis temperature upon hydroxyapatite particle size and density

In the synthesis of hydroxyapatite powders by spray pyrolysis, control of the particle size was investigated by varying the initial concentration of the precursor solution and the pyrolysis temperature. Calcium phosphate solutions (Ca/P ratio of 1.67) with a range of concentrations from 0.1 to 2.0 mol/L were prepared by dissolving calcium nitrate tetrahydrate and diammonium hydrogen phosphate in deionized water and subsequently adding nitric acid. Hydroxyapatite powders were then synthesized by spray pyrolysis at 900°C and at 1500°C, using these calcium phosphate precursor solutions, under the fixed carrier gas flow rate of 10 L/min. The particle size decreased as the precursor concentration decreased and the spray pyrolysis temperature increased. Sinterability tests conducted at 1100°C for 1 h showed that the smaller and denser the particles were, the higher the relative densities were of sintered hydroxyapatite disks formed from these particles. The practical implication of these results is that highly sinterable small and dense hydroxyapatite particles can be synthesized by means of spray pyrolysis using a low-concentration precursor solution and a high pyrolysis temperature under a fixed carrier gas flow rate.

Synthesis of a bioactive and degradable 70Poly(50Lactic-co-50Glycoric acid)/30(60SiO2-40CaQ) composite with dual pore structure

The bioactive and degradable 70poly(50lactic-co-50glycoric acid)/30(60SiO2-40CaO) composite, which had a dual pore structure, was synthesized by a gas expansion method. The bioactive 60SiO2-40CaO powders were prepared through a sol-gel method from tetraethyl orthosilicate and calcium nitrate tetrahydrate under nitric acidic condition followed by the heat treatment at 900°C for 1 h. The 70poly(50lactic-co-50glycoric acid)/30(60SiO2-40CaO) composite was then synthesized by a solvent casting method using 1,1,3,3-hexafluoro-2-propanol as a solvent. The disk-shaped 70poly(50lactic-co-50glycoric acid)/30(60SiO2-40CaO) composite was loaded into the custom-made high-pressure gas chamber and then carbon dioxide gas was introduced until achieving a final pressure of 20 MPa. After 1 day of loading, the carbon dioxide gas was released rapidly. The samples were observed by FE-SEM, the phase was evaluated by X-ray diffractometer, and its bioactivity was assessed in simulated body fluid. As a result, double pore structure was developed in the poly(50lactic-co-50glycoric acid) matrix after releasing the carbon dioxide gas and 60SiO2-40CaO particles were observed to place randomly among the poly(50lactic-co-50glycoric acid) matrix. After soaking in simulated body fluid for 1 week, flake-like low crystalline hydroxyl carbonate apatite was formed on the surface of the composite. The practical implication of the results is that it has promising potential for use as a scaffold material for bone tissue engineering due to its bioactivity and degradability.