Jeongran Lee

DNA profiling and genetic diversity of Korean soybean (Glycine max (L.) Merrill) landraces by SSR markers

Approximately 7,000 accessions of Korean soybean (Glycine max (L.) Merrill) landraces, largely composed of three collections, the Korea Atomic Energy Research Institute’s soybean (KAS), the Korean Crop Experiment Station’s soybean (KLS) and the Korean Agricultural Development and Technology Center’s soybean (KADTC) collections, have been conserved at the Rural Development Administration (RDA) genebank in Korea. The accessions within collections were classified based on their traditional uses such as sauce soybean (SA), sprouted soybean (SP), soybean for cooking with rice (SCR), and OTHERS. A total of 2,758 accessions of Korean soybean landraces were used to profile and to evaluate genetic structure using six SSR loci. A total of 110 alleles were revealed by at the six SSR loci. The number of alleles per SSR locus ranged from 9 to 39 in Satt187 and Satt_074, respectively. The number of alleles ranged from 87 in the KADTC collection to 96 in the KLS collection, and from 63 in the SCR group to 95 in the SP group. Nei’s average genetic diversity ranged from 0.68 to 0.70 across three collections, and 0.64 to 0.69 across the usage groups. The average between-group differentiation (Gst) was 0.9 among collections, and 4.1 among the usage groups. The similar average diversity among three collections implies that the genetic background of the three collections was quite similar or that there were a large number of duplicate accessions in three collections. The selection from the four groups classified based upon usage may be a useful way to select accessions for developing a Korean soybean landrace core collection at the RDA genebank. DNA profile information of accessions will provide indications of redundancies or omissions and aid in managing the soybean collection held at the RDA genebank. The information on diversity analysis could help to enlarge the genetic diversity of materials in breeding programs and could be used to develop a core collection.

Evaluation of Genetic Diversity of Red Pepper Landraces (Capsicum annuum L.) from Bulgaria Using SSR Markers

The amount of genetic variability of a species is essential for its survival and adaptation in different environments, and studies of genetic diversity using molecular markers are necessary to understand the genetic structure of a population and to orientate effective strategies of germplasm conservation. The aim of current study was to determine the SSR markers that can be used rapidly and reliably to evaluated the pepper of Bulgaria landraces, and applied the markers to assessment of introduce genetic diversity of the pepper germplasm. We used 22 polymorphic microsatellite markers to analysis of genetic diversity within 61 pepper collection of Bulgaria landraces germplasm, all SSR primers pairs produced 82 polymorphic and reproducible amplification fragments. Results showed a relatively low level of genetic diversity among pepper accessions from Bulgaria for both molecular and morphological markers. An average value of polymorphic information contents (PIC) were 0.334 with a range of 0.061 to 0.63. The mean values of observed (HO) and gene diversity (GD) were 0.383 and 0.154, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into three distinct groups according to genetic difference among the accessions. An average day of flowering time was 56.9 days with a range of 48 to 65 days. The average of fruit length and width were 6.9cm with a range 2.6 to 13.9cm, and 1.9cm with a range 0.5 to 4.2cm, respectively.

Study of the genetic diversity of Korean, Chinese and Japanese landraces of barley (Hordeum vulgare L.) using microsatellites

Study of the genetic diversity of Korean, Chinese and Japanese landraces of barley (Hordeum vulgare L.) using microsatellites Barley (Hordeum vulgare L.) is one of important winter cereals in the world and has been the subject of numerous genetic investigations. Studies of its genetic diversity based on germplasm have a significant impact on crop breeding and conservation of genetic resources. This study was conducted to reveal the genetic diversity in barley landraces from Korean, Chinese and Japanese populations and evaluate the discrimination ability of SSR markers, distributed uniformly throughout the barley genome. Seven SSR primers were used to screen a set of 737 diverse barley landraces from Korea, China and Japan. The observed number of alleles per locus (Na), the effective number of alleles (Ne), and the mean gene diversity (He) were 11.14, 3.6245 and 0.7048, respectively. The number of alleles per locus was highest in Chinese landraces (8.9 alleles), followed by Korean (8.6) and Japanese (6.4). In this regard, HVKASI primer may be useful to distinguish Japanese landraces from others, as this unique allele was only detected at 175 bp in Japanese landraces. The average value of genetic distance was D=0.935. The largest genetic distance (D=1.209) among the three regional (representing each country in general) populations was found between Korean and Japanese populations. Based on the UPGMA dendrogram, four major groups can be distinguished at the similarity value of 0.43. The scatter plot showed overlapping in the central part amongst 3 groups of barley landraces. SSR markers are a powerful tool to examine functional diversity. Rich barley gene pool can be very useful for meeting current and future challenges in barley raising.