Jerome V. Schnell

Stearic acid as plasma replacement for intracellular in vitro culture of Plasmodium knowlesi.

A chloroform extract of Cohns fraction IV-4 of human plasma successfully replaced whole fraction IV-4 for the intracellular in vitro culture of Plasmodium knowlesi. We are now able to report the successful replacement of monkey plasma by stearic acid.

Plasma Replacement for in vitro Culture of Plasmodium klowlesi

Of six fractions of human plasma tested, only Cohns fraction IV-4 was effective for the replacement of whole plasma from monkey (Macaca mulatta) for the in vitro culture of Plasmodium knowlesi. The effects observed on multiplication and morphology of parasites suggest a specific role of some substance or substances in fraction IV-4.

Effects of red cell extract on in vitro growth and multiplication of malarial parasites.

An extract of red blood cells was prepared from monkey blood. Red cell extract (RCE) of Aotus monkeys was beneficial for the in-vitro cultivation of 2 strains of P. falciparum. The active material(s) in Aotus RCE was not sedimented by centrifugation at 34,800 g for 1 hr. Rhesus monkey RCE improved the growth of P. knowlesi for in-vitro cultivation through 4 schizogonous cycles (88 hr).

In vitro cultivation of Plasmodium malariae.

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