Jerzy Molenda

The profiles of enterotoxin genes in Staphylococcus aureus from nasal carriers

Aims:  To evaluate the occurrence of enterotoxin genes in Staphylococcus aureus recovered from nasal carriers.

Characterization of borderline oxacillin-resistant Staphylococcus aureus isolated from food of animal origin.

In this study, the molecular characteristics of food-derived oxacillin-resistant Staphylococcus aureus were determined. Eight borderline oxacillin-resistant strains with MICs of 2 to 4 microg/ml were identified from 132 S. aureus isolates of food origin. One of the two isolates with a MIC of 4 microg/ml was methicillin-resistant determinant (mecA) gene positive, and the other six with MICs of 2 microg/ml were mecA negative. The mecA-positive isolate was classified as sequence type (ST)228, staphylococcal protein A (spa) type t041, and carried the staphylococcal cassette chromosome mec type I element. Two borderline oxacillin-resistant strains were classified as spa t008 and ST8, and the remaining five as spa t164 and ST20. The mecA-positive strain and four borderline oxacillin-resistant strains were found enterotoxigenic. The enterotoxin genes detected in these strains included selp, egc1, and sed-sej-selr. The borderline-resistant S. aureus isolates from a manually handled product, i.e., minced pork, were shown genetically related to strains associated with human infections. This suggests that humans can be considered as a source of contamination of this food with oxacillin-resistant S. aureus strains. The genotypes of the investigated milk borderline-resistant isolates were shown to occur not only in cows, but also in humans. Since manual handling is reduced in raw milk production, a human origin of S. aureus seems unlikely. Because knowledge of the genotypes of animal staphylococci is limited, more research is needed to address the question of the origin of antibiotic-resistant S. aureus strains in food.

Effect of heat exposure on invasiveness of Listeria monocytogenes strains.

The effect of a 54°C heat exposure lasting 20 and 60 minutes on the invasiveness of 10 Listeria monocytogenes strains in the exponential and stationary phases was investigated. It was shown that heat exposure significantly reduced the L. monocytogenes count. A maximal reduction of 5 log took place after 60 minutes of heating. No significant differences in survival between exponential and stationary phases were observed for most strains. Twenty minutes of heat exposure resulted in a reduction of the invasiveness of L. monocytogenes, which was more pronounced in the exponential-phase bacteria. Prolongation of heating to 60 minutes was shown to have a significant impact on the invasiveness of five stationary-phase strains. It was demonstrated that heat exposure influences the survival of L. monocytogenes strains as expected. Also, the invasiveness was significantly changed in a time- and growth phase-dependent manner. Low reductions of bacterial counts in milder conditions, that is, 20 minutes of heat, were accompanied by a decrease of the invasiveness of all L. monocytogenes strains. Prolongation of heating time to 60 minutes resulted in significant reduction of bacterial numbers. However, bacteria, especially those in the stationary phase, which survived this treatment can become more invasive.