Jessica C. Lee

The role of contingency awareness in single-cue human eyeblink conditioning

Single-cue delay eyeblink conditioning is presented as a prototypical example of automatic, nonsymbolic learning that is carried out by subcortical circuits. However, it has been difficult to assess the role of cognition in single-cue conditioning because participants become aware of the simple stimulus contingency so quickly. In this experiment (n = 166), we masked the contingency to reduce awareness. We observed a strong relationship between contingency awareness and conditioned responding, with both trace and delay procedures. This finding suggests that explicit associative knowledge and anticipatory behavior are regulated by a coordinated system rather than by functionally and neurally distinct systems.

Extinction and renewal of cue-elicited reward-seeking

Reward cues can contribute to overconsumption of food and drugs and can relapse. The failure of exposure therapies to reduce overconsumption and relapse is generally attributed to the context-specificity of extinction. However, no previous study has examined whether cue-elicited reward-seeking (as opposed to cue-reactivity) is sensitive to context renewal. We tested this possibility in 160 healthy volunteers using a Pavlovian-instrumental transfer (PIT) design involving voluntary responding for a high value natural reward (chocolate). One reward cue underwent Pavlovian extinction in the same (Group AAA) or different context (Group ABA) to all other phases. This cue was compared with a second non-extinguished reward cue and an unpaired control cue. There was a significant overall PIT effect with both reward cues eliciting reward-seeking on test relative to the unpaired cue. Pavlovian extinction substantially reduced this effect, with the extinguished reward cue eliciting less reward-seeking than the non-extinguished reward cue. Most interestingly, extinction of cue-elicited reward-seeking was sensitive to renewal, with extinction less effective for reducing PIT when conducted in a different context. These findings have important implications for extinction-based interventions for reducing maladaptive reward-seeking in practice.

Asexual reproduction and observations of sexual reproduction in the aspidochirotid sea cucumber Holothuria difficilis

Abstract The diminutive (<5.0 cm length) aspidochirotid sea cucumber Holothuria difficilis exhibits asexual propagation through transverse fission. The temporal pattern of fission was monitored in a population of H. difficilis at One Tree Island, Southern Great Barrier Reef (GBR), the first such study for this species. Asexual reproduction occurs year round with enhanced incidence of splitting in winter (May to September) when >50% of individuals were fission products. Inversely related to the temporal pattern of fission, the mean individual weight of H. difficilis increased from ca. 0.8 g in May to ca. 2.0 g in November. This doubling in individual weight over 6 months was largely due to regeneration. Gonads were only seen in large (4.0–5.0 cm length) H. difficilis. This species has a small egg (164 μm diameter). Although the population dynamics of H. difficilis at One Tree Island and elsewhere on the GBR is dominated by asexual clonal reproduction, the presence of gonads indicates that sexual reproduction does occur. Asexual reproduction of H. difficilis is compared with that documented for other tropical fissiparous aspidochirotids.

Peak shift and rules in human generalization.

Two experiments tested whether a peak-shifted generalization gradient could be explained by the averaging of distinct gradients displayed in subgroups reporting different generalization rules. Across experiments using a causal judgment task (Experiment 1) and a fear conditioning paradigm (Experiment 2), we found a close concordance between self-reported rules and generalization gradients using a continuous stimulus dimension (hue). Both experiments also showed an overall peak-shifted gradient after differential conditioning, but not after single cue conditioning. Importantly, the peak shift could be decomposed into linear and peaked gradients when participants were divided into rule subgroups. Our results highlight the need to consider individual differences in the rules that participants derive in human generalization studies and suggest that in some situations, peak shift may be a consequence of averaging across diverse rule subgroups.

Second-Order Conditioning and Conditioned Inhibition: Influences of Speed versus Accuracy on Human Causal Learning

In human causal learning, excitatory and inhibitory learning effects can sometimes be found in the same paradigm by altering the learning conditions. This study aims to explore whether learning in the feature negative paradigm can be dissociated by emphasising speed over accuracy. In two causal learning experiments, participants were given a feature negative discrimination in which the outcome caused by one cue was prevented by the addition of another. Participants completed training trials either in a self-paced fashion with instructions emphasising accuracy, or under strict time constraints with instructions emphasising speed. Using summation tests in which the preventative cue was paired with another causal cue, participants in the accuracy groups correctly rated the preventative cue as if it reduced the probability of the outcome. However, participants in the speed groups rated the preventative cue as if it increased the probability of the outcome. In Experiment 1, both speed and accuracy groups later judged the same cue to be preventative in a reasoned inference task. Experiment 2 failed to find evidence of similar dissociations in retrospective revaluation (release from overshadowing vs. mediated extinction) or learning about a redundant cue (blocking vs. augmentation). However in the same experiment, the tendency for the accuracy group to show conditioned inhibition and the speed group to show second-order conditioning was consistent even across sub-sets of the speed and accuracy groups with equivalent accuracy in training, suggesting that second-order conditioning is not merely a consequence of poorer acquisition. This dissociation mirrors the trade-off between second-order conditioning and conditioned inhibition observed in animal conditioning when training is extended.

Rule-based generalization and peak shift in the presence of simple relational rules

After discrimination learning between two stimuli that lie on a continuum, animals typically exhibit generalization on the basis of similarity to the physical features of the stimuli, often producing a peak-shifted gradient. However, post-discrimination generalization in humans usually resembles a monotonically increasing (e.g., linear) gradient that is better characterized as following a relational rule describing the difference between the stimuli. The current study tested whether rule-based generalization could be disrupted by reducing the applicability of a relational rule on test. We compared generalization following a difficult categorization task between a group who could use their rule consistently throughout test (Group Consistent), and a group who could only apply their rule effectively on 50% of test trials and thus could only use it inconsistently (Group Inconsistent). Across two experiments, a peak shift was found in the Inconsistent group and a monotonic gradient in the Consistent group. A post-hoc sequential analysis revealed that the Inconsistent group produced both peak-shifted and monotonic gradients as a function of whether or not the relevant rule was applicable on the previous trial. Reducing the applicability of a rule on test thus appeared to lead participants to revert to generalizing on the basis of similarity. Our results suggest that humans learn about the physical features of the stimuli alongside relational rules, and that rule- and similarity-based learning can interact in determining generalization.

The effect of encoding conditions on learning in the prototype distortion task

The prototype distortion task demonstrates that it is possible to learn about a category of physically similar stimuli through mere observation. However, there have been few attempts to test whether different encoding conditions affect learning in this task. This study compared prototypicality gradients produced under incidental learning conditions in which participants performed a visual search task, with those produced under intentional learning conditions in which participants were required to memorize the stimuli. Experiment 1 showed that similar prototypicality gradients could be obtained for category endorsement and familiarity ratings, but also found (weaker) prototypicality gradients in the absence of exposure. In Experiments 2 and 3, memorization was found to strengthen prototypicality gradients in familiarity ratings in comparison to visual search, but there were no group differences in participants’ ability to discriminate between novel and presented exemplars. Although the Search groups in Experiments 2 and 3 produced prototypicality gradients, they were no different in magnitude to those produced in the absence of stimulus exposure in Experiment 1, suggesting that incidental learning during visual search was not conducive to producing prototypicality gradients. This study suggests that learning in the prototype distortion task is not implicit in the sense of resulting automatically from exposure, is affected by the nature of encoding, and should be considered in light of potential learning-at-test effects.