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Publication
Featured researches published by Ji Ming.
药学学报 2018, Vol. 5, Pages 749-753 | 2018
季鸣; Ji Ming; 薛妮娜; Xue Nina; 黄蕊; Huang Rui; 刘羿晨; Liu Yi-chen; 金晶; Jin Jing; 陈晓光; Chen Xiaoguang
IL-6/JAK/STAT3是细胞内调节细胞生长、存活和分化的重要信号通路，这条信号通路也控制着T淋巴细胞的分化和激活。IL-6/JAK/STAT3信号通路的异常与自身免疫性疾病和肿瘤密切相关，已经成为药物研发的热门靶点。本研究利用Invivogen公司稳定表达IL-6受体且可与STAT3结合的分泌胚胎碱性磷酸酶（secreted embryonic alkaline phosphatase，SEAP）报告基因的HEK-Blue IL-6细胞，给予IL-6刺激下分泌SEAP，通过与QUANTI-Blue反应，在655 nm检测SEAP的生成量，评价化合物对IL-6刺激的STAT3信号通路的抑制作用。结果表明，IL-6可特异性地激活HEK-Blue IL-6细胞。在细胞数为5×104个/孔、1 ng·mL-1浓度IL-6刺激20 h，并于QUANTI-Blue反应1 h时检测OD655吸光度值为最佳反应条件。在筛选的14个天然产物中，牛蒡子苷元、隐丹参酮和姜黄素具有很强的抑制STAT3信号通路的活性，IC50值分别为1.28、2.96和6.61 μmol·L-1。该HEK-Blue IL-6细胞模型适用于IL-6/JAK/STAT3信号通路抑制剂的筛选。
American Journal of Translational Research | 2017
Jin Jing; Chen Xiaoguang; Lai Fangfang; Xue Nina; Wang Dongjie; Ji Ming
药学学报 2018, Vol. 6, Pages 938-943 | 2018
季鸣; Ji Ming; 姚海平; Yao Haiping; 周洁; Zhou Jie; 金晶; Jin Jing; 王丽嫄; Wang Liyuan; 来芳芳; Lai Fangfang; 薛妮娜; Xue Nina; 徐柏玲; Xu Bailing; 陈晓光; Chen Xiaoguang
Archive | 2017
Feng Zhiqiang; Chen Xiaoguang; Zhang Lijing; Yang Yang; Lai Fangfang; Ji Ming; Zhou Chuan; Zhen Yi; Xue Nina; Li Ling
Archive | 2017
Feng Zhiqiang; Chen Xiaoguang; Yang Yang; Zheng Yi; Lai Fangfang; Ji Ming; Zhou Chuan; Zhang Lijing; Wang Ke; Xue Nina; Li Ling
Archive | 2017
Xu Bailing; Chen Xiaoguang; Zhou Jie; Ji Ming; Yao Haiping; Zhou Qin
Archive | 2017
Feng Zhiqiang; Chen Xiaoguang; Zhang Lijing; Yang Yang; Lai Fangfang; Ji Ming; Zhou Chuan; Zheng Yi; Xue Nina; Li Ling
Archive | 2017
Feng Zhiqiang; Chen Xiaoguang; Yang Yang; Zhou Chuan; Lai Fangfang; Ji Ming; Jin Xiaofeng; Xue Nina; Zheng Yi; Chen Hao; Li Ling
Archive | 2017
Xu Bailing; Chen Xiaoguang; Yao Haiping; Ji Ming; Jin Jing; Zhou Jie; Wang Ke; Zhao Dalong
Zhongguo Yaowu Huaxue Zazhi | 2016
Zhou Jie; Zhu Zhixiang; Ji Ming; Cao Ran; Chen Xiaoguang; Xu Bailing
Collaboration
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