Ji Yeon Byun

Expression of IL-10, TGF-β1 and TNF-α in Cultured Keratinocytes (HaCaT Cells) after IPL Treatment or ALA-IPL Photodynamic Treatment

BACKGROUND Depending on the light dose and concentration of photosensitizer for photodynamic treatment (PDT), a multitude of dose-related events are demonstrable in PDT-treated cells. Sublethal doses may result in the alteration of cytokine release and consequently modify immune actions, rather than cause cell death. OBJECTIVE The purpose of this study was to investigate cytokine expression in cultured HaCaT cells after intense pulse light (IPL) treatment or PDT utilizing 5-aminolevulinic acid (ALA) and IPL at sublethal doses. METHODS Cultured HaCaT cells were treated with either IPL only (4, 8 and 12 J/cm(2)) or ALA-IPL PDT (100micromol/L of ALA; 0, 4, 8, and 12 J/cm(2) of IPL). The expression of IL-10, TGF-beta(1) and TNF-alpha was investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. RESULTS IL-10 protein increased up to 5.95-fold after IPL treatment and up to 2.85-fold after PDT. TGF-beta(1) mRNA and protein showed slight increases after both IPL treatment and PDT, of which the latter induced slightly larger increases. TNF-alpha mRNA and protein showed no induction or reduction after PDT. CONCLUSION Increased expressions of IL-10 and TGF-beta(1) was observed after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses, and that of TGF-beta(1) may be related to the therapeutic effect for psoriasis. The finding that IL-10 induction was more marked after IPL treatment than after PDT suggests that other mechanisms than IL-10 induction in keratinocytes after PDT may participate in the anti-inflammatory effect of PDT.

Nickel and cobalt release from jewellery and metal clothing items in Korea

In Korea, the prevalence of nickel allergy has shown a sharply increasing trend. Cobalt contact allergy is often associated with concomitant reactions to nickel, and is more common in Korea than in western countries.

Fexofenadine-Induced Urticaria

Fexofenadine (Allegra® 180) is a second-generation antihistamine. It is widely used as anti-allergic drug, which suppresses various allergic reactions mediated by histamines. A few cases of H1-antihistamine-induced urticaria have been reported. Herein, we report a rare case of fexofenadine-induced urticaria which was confirmed by a prick test, oral provocation test, and flow cytometry assisted-basophil activation test.

Generalized Granuloma Annulare in Infancy Following Bacillus Calmette-Guerin Vaccination

Generalized granuloma annulare (GGA) is a rare benign granulomatous dermatosis characterized by disseminated necrobiotic dermal papules. Histologically, it presents as a lymphohistiocytic granuloma associated with varying degrees of connective tissue degeneration. It usually occurs in adults and rarely affects infants. Herein, we report an interesting case of GGA which occurred in a 3 month-old girl in association with Bacillus Calmette-Guerin vaccination.

Occupational airborne contact dermatitis caused by trifluoroacetic acid in an organic chemistry laboratory

A 24-year-old Korean man had a 6-month history of recurrent rash on his neck and arms. He was otherwise healthy, and started his job at an organic chemistry laboratory 18 months previously. He reported that exposure to organic solvents or acidic agents, especially TFA, at the workplace caused him skin problems. On examination, pruritic, excoriated and lichenified patches were seen on the forehead, neck, and forearms. Patch tests were performed with the TRUE Test® (SmartPractice, Hillerod, Denmark), a cosmetic series (Chemotechnique Diagnostics, Vellinge, Sweden), and five chemicals (dichloromethane 1% pet., tetrahydrofuran 1% pet., pyridine 1% aqua, piperidine 1% aqua, and TFA 1%. aqua). The chemicals were brought in by the patient and prepared for the patch tests in our clinic. Patch tests were placed on the upper back, and readings were performed on D2 and D4, according to International Contact Dermatitis Research Group guidelines. There were positive reactions to TFA at D2 and D4, and positive reactions to benzyl alcohol and phenoxyethanol on D4. After informed consent had been obtained, 5 normal control volunteers were patch tested with TFA 1%, 0.1% and 0.01% aqua; no positive reactions were observed.

Granulomatous reaction after BCG vaccination histologically mimicking reticulohistiocytoma

Bacilli Calmette-Guerin (BCG) vaccine is made from attenuated strains of Mycobacterium bovis and is the only available vaccine against tuberculosis. The World Health Organization recommends that in countries with a high burden of tuberculosis, a single dose of BCG vaccine should be given to all healthy infants as soon as possible after birth [1]. In Korea, BCG vaccine is a part of the national childhood immunization program, given to all neonates at 0∼4 weeks.A 35-month-old healthy Korean boy visited [...]

Multiple myeloma presenting with acquired cutis laxa and primary systemic amyloidosis

654 EJD, vol. 27, n◦ 6, November-December 2017 1. Wollina U. Nodular skin reactions in eyebrow permanent makeup: two case reports and an infection by Mycobacterium haemophilum. J Cosmet Dermatol 2011; 10: 235-9. 2. Wenzel SM, Welzel J, Hafner C, Landthaler M, Bäumler W. Permanent make-up colorants may cause severe skin reactions. Contact Dermatitis 2010; 63: 223-7. 3. Morales-Callaghan AM Jr, Aguilar-Bernier M Jr, Martínez-García G, Miranda-Romero A. Sarcoid granuloma on black tattoo. J Am Acad Dermatol 2006; 55: 71-3. 4. Incel Uysal P, Gurel MS, Behzatoglu K. A Tattoo-associated complication: foreign body granulomatous reaction. Am J Dermatopathol 2016; 38: 936-7. 5. Godinho MM, Aguinaga F, Grynszpan R, et al. Granulomatous reaction to red tattoo pigment treated with allopurinol. J Cosmet Dermatol 2015; 14: 241-5. 6. Serup J, Bäumler W. Guide to treatment of tattoo complications and tattoo removal. Curr Probl Dermatol 2017; 52: 132-8. 7. Antonovich DD, Callen JP. Development of sarcoidosis in cosmetic tattoos. Arch Dermatol 2005; 141: 869-72. 8. Ohtsuka M, Natsuko M, Toshiyuki Y. Sarcoidal granuloma presenting on tattoo: a report of a Japanese female patient and a review of Japanese published work. Sarcoidosis Vasc Diffuse Lung Dis 2016; 33: 83-9. 9. Ravits HG. Allergic tattoo granuloma. Arch Dermatol 1962; 86: 287-9.

A Case of Cutaneous Protothecosis in an Immunocompetent Patient

Dear Editor: Protothecosis is a rare infection, caused by the genus Prototheca, achlorophyllous algae which are saprophytes in soil, and occasionally affect dogs and cats1. It is believed that they may infect humans by traumatic inoculation2. A 74-year-old woman visited our clinic with prickling erythematous umbilicated papules on the anterior neck and upper chest which developed 2 months prior on top of an erythematous telangiectatic patch which had been present for 2 years (Fig. 1A, B). She had been treated at another clinic. with topical corticosteroids for 2 years for suspected eczema aggravated by rubbing. She had no history of trauma or other medical disease. She had lived with a pet dog for 18 years. Fig. 1 (A) Several crusted papules on an erythematous telangiectactic patch on the anterior neck and upper chest. (B) Close-up view

A Case of Allergic Contact Dermatitis Due to DuoDERM Extrathin

Over the past years, hydrocolloid dressings have been introduced routinely in the treatment of various types of wounds. They provide a moist environment promoting autolytic debridement, and stimulate angiogenesis. However, long-term application often leads to inflammation of the skin in the immediate area of the ulcer, causing irritant dermatitis in many cases, but sometimes also leads to contact sensitization. A 32 year-old woman burnt herself by an iron, and presented to our clinic and was treated with Duoderm extrathin®. Nine days later, she again presented with an erythematous oozing patch with edema, and allergic contact dermatitis was suspected. A patch test (TRUE test) was performed and a positive reaction to colophonium was obtained. Duoderm extrathin® contains hydrogenated rosin (colophonium) as the tackifying agent, so we could diagnose this case as allergic contact dermatitis due to the hydrogenated rosin in Duoderm extrathin®. We report another case of allergic contact dermatitis due to Duoderm extrathin® in a 32 year-old woman.

A Case of Dedifferentiated LiposarcomaThat Developed in the Dermis.

Dedifferentiated liposarcoma is a variant of liposarcoma, and this is characterized by the coexistence of well-differentiated liposarcoma with areas of poorly differentiated, non-lipogenic tumor and this is also known to be associated with more aggressive behavior. Dedifferentiated liposarcoma occurs principally in the retroperitoneum or the deep soft tissue of limbs, but it can also occur in subcutaneous locations. We report here on a peculiar case of dedifferentiated liposarcoma that developed in the dermis, which is an exceedingly rare location for this type of tumor. The occurrence of this tumor in the dermis made it easy to surgically remove and monitor for recurrence, and we expect this patient to have a better prognosis than that of a patient with dedifferentiated liposarcoma located in the retroperitoneum or deep soft tissue.