Jia-Ming Liu

A colorimetric probe for online analysis of sulfide based on the red shifts of longitudinal surface plasmon resonance absorption resulting from the stripping of gold nanorods

A gold nanorods (GNRs) nonaggregation-based colorimetric probe has been developed for the detection of S(2-) based on that the longitudinal surface plasmon resonance absorption wavelength (LPAW) of GNRs red shifts (Δλ) and the color of the solution distinctly changes on account of the faster stripping of GNRs along longitudinal axis than transverse axis in the process of GNRs reacting with S(2-) ions to form Au(2)S complexes on the GNRs surfaces. The GNRs probe exhibits highly sensitive and selective response toward S(2-) with a wide linear range from 10.0 to 10000.0 μM. The proposed colorimetric probe can be used to visibly detect S(2-) in water samples on line in 15 min with the results agreeing well with those of the optical sensor, showing its great practicality. Moreover, the detection mechanism of the probe is also discussed.

Ultra-sensitive non-aggregation colorimetric sensor for detection of iron based on the signal amplification effect of Fe3+ catalyzing H2O2 oxidize gold nanorods

Fe(3+) can catalyze H2O2 to oxidize along on the longitudinal axis of gold nanorods (AuNRs), which caused the aspect ratio of AuNRs to decrease, longitudinal plasmon absorption band (LPAB) of AuNRs to blueshift (Δλ) and the color of the solution to change obviously. Thus, a rapid response and highly sensitive non-aggregation colorimetric sensor for the determination of Fe(3+) has been developed based on the signal amplification effect of catalyzing H2O2 to oxidize AuNRs. This simple and selective sensor with a wide linear range of 0.20-30.00 μM has been utilized to detect Fe(3+) in blood samples, and the results consisted with those obtained by inductively coupled plasma-mass spectroscopy (ICP-MS). Simultaneously, the mechanism of colorimetric sensor for the detection of Fe(3+) was also discussed.

Determination of trace glucose and forecast of human diseases by affinity adsorption solid substrate-room temperature phosphorimetry based on triticum vulgaris lectin labeled with dendrimers-porphyrin dual luminescence molecule

In this paper, 3.5-generation polyamidoamine dendrimers (3.5G-D)-porphyrin (P) dual luminescence molecule (3.5G-D-P) was developed as a new phosphorescence-labeling reagent. Meanwhile, the room temperature phosphorescence (RTP) characteristics of 3.5G-D-P and its product of labeling triticum vulgaris lectin (WGA) on the surface of polyamide membrane (PAM) were studied. Results showed that in the presence of heavy atom perturber LiAc, 3.5G-D and P of 3.5G-D-P molecule could emit strong and stable RTP on the PAM. And the Tween-80 would spike thoroughly the phosphorescence signal of 3.5G-D and P; moreover, specific affinity absorption (AA) reaction between the products (Tween-80-3.5G-D-P-WGA) of WGA labeled with Tween-80-3.5G-D-P and glucose (G) was carried out. The products of the AA reaction could keep good RTP characteristics of 3.5G-D and P dual luminescence molecule, and the DeltaI(p) was linear correlation to the content of G. According to the facts above, a new method of affinity adsorption solid substrate-room temperature phosphorimetry (AA-SS-RTP) for the determination of trace G was established, basing on WGA labeled with Tween-80-3.5G-D-P dual luminescence molecule. The detection limit of this method was 0.13fgspot(-1) (1.7x10(-12)moll(-1), 3.5G-D) and 0.14fgspot(-1) (2.2x10(-12)moll(-1), P). Determination of G in human serum using excitation/emission wavelength of either 3.5G-D or P, the result was coincided with enzyme-linked immunosorbent assay (ELISA). Not only the sensitivity and accuracy of this method were higher, but also the flexibility of AA-SS-RTP was obviously improved and the applicability was wider.

A highly sensitive coupling technique for the determination of trace quercetin based on solid substrate room temperature phosphorimetry and poly (vinyl alcohol) complex imprinting

Al(3+) could react with quercetin (Q) to form [AlQ](3+) complex which could be used as a template for the preparation of poly (vinyl alcohol)-[AlQ](3+) complex imprinting (PVA-C-I). The [AlQ](3+) not only had good matching ability and selectivity with the cavity of PVA-C-I, but also could react with the fluorescein isothiocyanate anion (FITC(-)) on the outside of cavity by electrostatic interaction to form ion-association complex [AlQ](3+)·[(FITC)(-)](3). The ion-association complex could emit strong and stable room temperature phosphorescence (RTP) on polyamide membrane (PAM) and the ΔI(p) of the system had linear relationship with the content of Q, showing the highly selective identification of PVA-C-I to Q. Thus, a new coupling technique for the determination of trace Q based on solid substrate room temperature phosphorimetry and poly (vinyl alcohol) complex imprinting (PVA-C-I-SSRTP) was established. The linear range and limit of detection (LOD) of this method were 0.010-2.0 (×10(-12) g mL(-1)) and 2.0×10(-14) g mL(-1), respectively, showing wide linear range and high sensitivity of PVA-C-I-SSRTP. This method was used to determine the content of Q in waste water, and the results are consistent with those by spectrofluorimetry. Meanwhile, the mechanism for the determination of Q using PVA-C-I-SSRTP was also discussed.

Solid substrate-room temperature phosphorimetry for the determination of residual clenbuterol hydrochloride based on the catalysis of sodium periodate oxidizing eosine Y

Clenbuterol hydrochloride (CLB) could catalyze NaIO(4) oxidation of eosine Y (R), which caused the room temperature phosphorescence (RTP) signal of R to quench sharply. The DeltaI(P) (= I(P2)-I(P1), I(P2) was RTP intensities of reagent blank and I(P1) was RTP intensities of test solution) of the system was directly proportional to the content of CLB. According to that academic thought, a new solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace CLB has been established. This method has high sensitivity (detection limit (LD): 0.021 zg spot(-1), corresponding concentration: 5.2x10(-20) g mL(-1)) and good selectivity (Er = +/-5%, interfering species were of no interference). It has been applied to the determination of residual CLB in the practical samples. The results were verified using HPLC and GC/MS methods. The reaction mechanism of catalytic SS-RTP for the determination of residual CLB was also discussed.

Exploitation of phosphorescent labelling reagent of fullerol-fluorescein isothiocyanate and new method for the determination of trace alkaline phosphatase as well as forecast of human diseases.

A new phosphorescent labelling reagent consisting of fullerol, fluorescein isothiocyanate and N,N-dimethylaniline (F-ol-(FITC)(n)-DMA) was developed. The mode of action is based on the reactivity of the active -OH group in F-ol with the -COOH group of FITC to form an F-ol-(FITC)(n)-DMA complex containing several FITC molecules. F-ol-(FITC)(n)-DMA increased the number of luminescent molecules in the biological target of WGA-AP-WGA-F-ol-(FITC)(n)-DMA (WGA and AP are wheat germ agglutinin and alkaline phosphatase, respectively) which improved the sensitivity using solid substrate room temperature phosphorimetry (SSRTP) detection. The proposed method provided high sensitivity and strong specificity for WGA-AP. The limit of detection (LD) was 0.15 ag AP spot(-1) for F-ol and 0.097 ag AP spot(-1) for FITC in F-ol-(FITC)(n)-DMA, which was lower than the method using single luminescent molecules of F-ol-DMA and FITC-DMA to label WGA (0.20 ag AP spot(-1) for F-ol-DMA and 0.22 ag AP spot(-1) for FITC-DMA). Results for the determination of AP in human serum were in good agreement with those obtained by enzyme-linked immunosorbent assay. The mechanism of F-ol-(FITC)(n)-DMA labelling of WGA was discussed.

Determination of Trace Alkaline Phosphatase by Solid‐Substrate Room‐Temperature Phosphorimetry Based on Triticum vulgare Lectin Labeled with Fullerenol

Fullerenol (F) shows a strong and stable room‐temperature phosphorescence (RTP) signal on the surface of nitrocellulose membrane (NCM) at λ

Affinity adsorption solid substrate-room temperature phosphorimetry for the determination of alkaline phosphatase

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Fullerol-fluorescein isothiocyanate phosphorescent labeling reagent for the determination of glucose and alkaline phosphatase

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Determination of Trace Deoxyribonucleic Acid Based on a Room Temperature Phosphorescent Probe of Alizarin Red-piperidine Self-ordered Ring

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