Jiangyun Liu

New triterpenoid saponins from Patrinia scabiosaefolia.

Phytochemical investigation of the methanol extract from the whole plants of Patrinia scabiosaefolia Fisch. resulted in the isolation of four new triterpenoid saponins (1-4) along with six known compounds (5-10). On the basis of spectroscopic and chemical methods, the structures of the new compounds were established as 3-O-β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl-12β,30-dihydroxy-olean-28,13β-olide (1), 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl-12β,30-dihydroxy-olean-28,13β-olide (2), 3-O-β-D-xylopyranosyl-(1→2)-β-D-glucopyranosyl-12β, 30-dihydroxy-olean-28,13β-olide (3), and 3-O-β-D-glucopyranosyl-(1→4)-β-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-β-D-xylopyranosyl-oleanolic acid 28-O-β-D-glucopyranoside (4), respectively. Compounds 1-3 possess a novel 12β,30-dihydroxy-olean-28,13β-lactone aglycone and a 12β-hydroxy substituent that is rarely found in this kind of triterpenoid saponin.

Controlled acetylation of water-soluble glucomannan from Bletilla striata.

Glucomannans from Bletilla striata (bletillan) were used as excipient for controlled deliveries of drugs, genes and tissue engineering. In the present study, a controlled acetylation method was developed to improve water solubility of bletillan 70 (BT) firstly, by reacting with acetic anhydride (AA) in N,N-dimethylformamide solvent. The preparation parameters, such as reaction temperature, reaction time and molar ratio of BT/AA, were optimized based on degrees of acetyl group in addition. IR and (1)H NMR spectra were applied to elucidate the reaction process and substitution pattern, which indicated that the acetylation took place at C-6 and C-2 of the hexose units in a ratio of 2:1, with DS up to 0.83. Relative viscosity analysis revealed that the resulted products had improved water solubilities. This novel method is simple, economic and easily controlled.

Flavone C-glycosides from the flowers of Trollius chinensis and their anti-complementary activity

Phytochemical investigation of ethanol extract from the flowers of Trollius chinensis Bunge resulted in the isolation of two new flavone C-glycosides (1–2), along with 10 known compounds (3–12). The structures of the new compounds were established as 6‴-(3-hydroxy-3-methylglutaroyl)-2″-O-β-d-galactopyranosyl orientin (1) and 6‴-(3-hydroxy-3-methylglutaroyl)-2″-O-β-d-galactopyranosyl vitexin (2) on the basis of various spectroscopic analysis (including different 1D and 2D NMR spectroscopies, high-resolution electrospray ionization mass spectrometry) and chemical evidences. Bioassay showed that eight flavonoids inhibited complement activation on the classic pathway in vitro, with their IC50 values ranging from 0.88 to 4.02 mM, which may contribute to the applications of the herb in treatment of acute respiratory distress syndrome, etc.

Complete 1H and 13C data assignments of two new guaianolides isolated from Ainsliaea fragrans.

Two new guaianolides, 8α‐hydroxy‐11α,13‐dihydroglucozaluzanin C and 11α,13‐dihydroglucozaluzanin C, were isolated from Ainsliaea fragrans, along with two known guaianolides, 8α‐hydroxy‐11α,13‐dihydrozaluzanin C and glucozaluzanin C. The structures of the new compounds were unambiguously established by HR‐ESI‐MS, one‐dimensional (1D) (DEPT), two‐dimensional (2D) (1H1H COSY, HSQC, HMBC, ROESY) NMR experiments and by comparison with structurally related compounds. The known compounds were identified by comparison of spectral data with published references. Some NMR data of the known compounds were reported for the first time. Copyright

New triterpenoid saponins from Patrinia scabiosifolia

Phytochemical investigation of methanol extract from the whole plants of Patrinia scabiosifolia Fisch. resulted in the isolation of three new triterpenoid saponins (1–3) along with twelve known triterpenoids (4–15). The structures of the new compounds were established as 11α, 12α-epoxy-3-O-β-d-xylopyranosyl-olean-28, 13β-olide (1), 11α, 12α-epoxy-3-O-β-d-xylopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 2)-β-d-xylopyranosyl-olean-28, 13β-olide (2), and 3-O-β-d-xylopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 2)-β-d-xylopyranosyl oleanolic acid 28-O-β-d-glucopyranoside (3) on the basis of various spectroscopic analyses (including different 1D and 2D NMR spectroscopies and high-resolution electrospray ionization mass spectrometry) and chemical evidences.