Jianyu He

Identification and analysis of icCu/Zn-SOD, Mn-SOD and ecCu/Zn-SOD in superoxide dismutase multigene family of Pseudosciaena crocea

Superoxide dismutases (SODs) belong to a significant and ubiquitous family of metalloenzymes for eliminating excess reactive oxygen species (ROS). In this paper, the complete open reading frames (ORFs) of intracellular Cu/Zn-SOD (icCu/Zn-SOD), Mn-SOD and extracellular Cu/Zn-SOD (ecCu/Zn-SOD) were identified from the large yellow croaker (Pseudosciaena crocea, designated as LycSOD1, LycSOD2 and LycSOD3). The sequences were 465 bp, 678 bp and 645 bp (GenBank accession no. KJ908287, KJ908285 and KJ908286), encoding 154, 225 and 215 amino acid (aa) residues respectively. The deduced aa sequences of LycSOD1, LycSOD2 and LycSOD3 shared high identity to the known icCu/Zn-SODs, Mn-SODs and ecCu/Zn-SODs with BLASTp and Phylogenetic analysis. Two conserved Cu-/Zn-binding sites (H-44, H-47, H-64, H-121 for Cu binding and H-64, H-72, H-81, D-84 for Zn binding in LycSOD1, H-98, H-100, H-115, H-164 for Cu binding and H-115, H-163, H-166, D-169 for Zn binding in LycSOD3) and one conserved manganese coordinating sites (H-57, H-101, D-186, H-190 in LycSOD2) were identified. The total length of DNA sequences of LycSOD1, LycSOD2 and LycSOD3 were 3447 bp, 3387 bp and 3886 bp respectively, and there were 4 introns and 5 exons in Cu/Zn-SODs (LycSOD1 and LycSOD3), but only 3 exons and 2 introns in LycSOD3. Spatial expression analysis indicated the highest mRNA expression of three SODs all appeared in liver among eight detected tissues, the highest expression level was LycSOD1, then LycSOD2 and the lowest was LycSOD3 for almost each tissue. The expression of LycSOD1, LycSOD2 and LycSOD3 mRNA were all up-regulated in liver after Vibrio alginolyticus stimulation. The temporal expression peak of LycSOD1 and LycSOD2 were around 9-fold and 8-fold compared to control respectively, whereas, LycSOD3 got the highest level at 48 h post-injection (about 4.2-fold). All the results gave several new and useful evidences for further understanding the regulatory mechanism of superoxide dismutases in the innate immune system of sciaenidae fish.

The SREC-I and SREC-II associated with epidermal growth factor in scavenger receptor family are the potential regulative transmembrane receptors in Larimichthys crocea

In innate immunity, the regulation of the immunologic gene expression plays a vital role in defense against pathogenic threat. The class F scavenger receptors (SCARFs), a kind of crucial immunologic type I transmembrane receptors, mainly involve in the signal transmission and eliminating pathogens in host immune system. In this study, the SREC-I and SREC-II of SCARFs in Larimichthys crocea (designated as LycSREC1 and LycSREC2 respectively) were first identified, the potential genetic locus relationships with other species were depicted and the features of gene expression after Vibrio alginolyticus stimulation were tested. The results demonstrated that the complete ORF sequences of two candidates were 3024 bp and 2832 bp (KM884873 and KM884874) respectively including some important domains and motifs, such as EGF/EGF-like domains, TRAF2-binding consensus motif, generic motif and atipical motif. The gene location maps and genetic locus interpreted that the DNA sequences of LycSREC1 and LycSREC2 were 7603 bp and 4883 bp, and some locus had changed compared with human being, but three more crucial genetic locus were conservative among ten species. Furthermore, quantitative real-time PCR (qRT-PCR) analysis indicated that the highest mRNA expression of LycSREC1 and LycSREC2 were both in liver among eight detected tissues, and their expression were up-regulated by V. alginolyticus stimulation. All these findings would contribute to better understanding the biologic function of SCARFs in defending against pathogenic bacteria challenge and further exploring the innate immune of sciaenidae fish.

Abundant members of Scavenger receptors family and their identification, characterization and expression against Vibrio alginolyticus infection in juvenile Larimichthys crocea

Scavenger receptors (SRs) are crucial pattern recognition receptors (PRRs) to defense pathogen infection in fish innate immunity. In this paper, some members in SRs family of Larimichthys crocea were identified, including eight genes in the class A, B, D and F families. (G + C) % of all SRs members held 51% ∼ 59%, and these genes were no obvious codon bias by analyzing the distribution of A-, T-, G- and C-ended codons. The order of Enc for all SRs members by sequencing was LycCD68 > LycSCARA5 > LycSCARB1 > LycCD163 > LycMARCO > LycSREC1 > LycSCARA3 > LycSREC2. Moreover, different lengths and numbers of exons and introns led to the diverse mRNAs and respective functional domains or motifs, for example, an optional cysteine-rich (SRCR) domain in LycMARCO and LycSCARA5, an epidermal growth factor (EGF) and EGF-like domain in LycSREC1 and LycSREC2. The sub-cellular localization demonstrated SRs members mainly located in plasma membrane or extracellular matrix. Further, all of the SRs members in L. crocea were almost low expressed in heart, gill and intestine, whereas high in spleen and liver. After stimulation by Vibrio alginolyticus, the class A and F families were induced significantly, but the class B and D families expressed less even none after pathogenic infection. All the findings would pave the way to understand not only the evolution of the SR-mediated immune response, but also the complexity of fish immunity.

Corrigendum to “Anti-infective mannose receptor immune mechanism in large yellow croaker (Larimichthys crocea)” [Fish Shellfish Immunol. 54 (2016 Jul) 257–265]

Corrigendum to “Anti-infective mannose receptor immune mechanism in large yellow croaker (Larimichthys crocea)” [Fish Shellfish Immunol. 54 (2016 Jul) 257e265] Xiangli Dong , Jiji Li a, b, , Jianyu He , Wei Liu , Lihua Jiang , Yingying Ye , Changwen Wu a, * a National Engineering Research Center of Maricultural Facilities of China, College of Marine Science and Technology, Zhejiang Ocean University, Haida South Road 1, 316022, Zhoushan, Zhejiang, PR China b Universita degli Studi di Napoli “Federico II”, Parco Gussone 1, 80055, Portici, Naples, Italy c Enea CR Portici, P. le E. Fermi, 1, 80055, Portici, Naples, Italy