Jiayi Wang

Association of Interleukin-8 Gene Polymorphisms and Haplotypes with Oral Lichen Planus in a Chinese Population

Interleukin-8 (IL-8), a CXC chemokine with multiple biological functions, plays an important role in the pathogenesis of oral lichen planus (OLP). The aim of this study was to investigate the association of single nucleotide polymorphisms (SNPs) of IL-8 gene with OLP in a Chinese population. Four SNPs of the IL-8 gene at positions −845 T/C (rs2227532), −738 T/A, −251 A/T (rs4073) and +781 C/T (rs2227306) were analyzed in 109 patients with OLP and 101 normal controls using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method. The data revealed that the −251 AA genotype and −251 A allele frequency was significantly lower in the erosive OLP (eOLP) group than in the control group (P = 0.012 and P = 0.031, respectively). Haplotype analysis revealed that the −251 A/+781 C haplotype frequency was lower in the eOLP group than in the control group (P = 0.029) while the −251 T/+781 C haplotype frequency was higher in the eOLP patients than in the healthy controls (P = 0.028). The study suggests that the IL-8 polymorphisms may be associated with the severity of OLP in this Chinese cohort.

Association between -308 G/A polymorphism in TNF-α gene and lichen planus: a meta-analysis.

BACKGROUND Different studies have conflicting opinions on the association between the -308 G/A polymorphism in TNF-α gene and genetic risk of lichen planus (LP). OBJECTIVE The purpose of this meta-analysis is to comprehensively evaluate interactions on this polymorphism and LP risk. METHODS A meta-analysis was employed to assess genetic risk of -308 G/A polymorphism in TNF-α gene for lichen planus. Odds ratios (ORs) with 95% confidence intervals (CIs) were also included. RESULTS Five studies including 8 comparisons were involved in this meta-analysis. The result showed that no association was found between this polymorphism and LP risk in combined analyses (OR=1.42 and 95% CI=0.85-2.37, P=0.180 for AA+GA vs. GG model). In the subgroup analysis by subtypes of LP (cutaneous LP and OLP) and OLP (eOLP, neOLP and mixed), no significant connections of risks were obtained from the two groups for AA+GA vs. GG comparison. In the subgroup analysis by ethnicity, significant increased OLP risks were found among population with mixed ethnicity (OR=3.26, 95%CI=1.46-7.26, P=0.004), but not in Asians (OR=1.19, 95%CI=0.69-2.05, P=0.528) and Caucasians (OR=1.32, 95%CI=0.41-4.27, P=0.645) for AA+GA vs. GG comparison. For the population presence or absence of hepatitis C virus (HCV) infection, significant increased risk of OLP was found among patients without HCV infection (OR=2.16, 95%CI=1.05-4.43, P=0.037), but not in LP-HCV +ve patients (OR=0.48, 95%CI=0.13-1.69, P=0.251) and mixed HCV status LP patient (OR=1.24, 95%CI=0.62-2.50, P=0.546). However, the negative results could have been biased because some included papers were lack of some information, which mainly related to HCV-status and clinical variety. That is the limitation of this meta-analysis. CONCLUSIONS The -308 G/A polymorphism may be a risk factor for OLP patients without HCV infection and those with mixed ethnicity. More studies are needed to validate these associations.

The association between hypoxia-inducible factor-1 α gene G1790A polymorphism and cancer risk: a meta-analysis of 28 case–control studies

PurposeHypoxia-inducible factor-1 (HIF-1) is a key transcription factor that regulates the cellular adaptation to hypoxia. HIF-1α gene single nucleotide polymorphisms (SNPs) are implicated to be associated with cancer risks. However, results from the published studies remained inconclusive. The aim of this study is to investigate the relationship of HIF-1α gene G1790A polymorphism with cancer using meta-analysis.MethodsA comprehensive search in Pubmed, EMBASE and China National Knowledge Infrastructure (CNKI) was conducted to identify all publications on the association between this polymorphism and cancer until December 13, 2013. Odds ratios (OR) with 95% confidence intervals (95% CI) were used to evaluate the strength of this association. Association between lymph node metastasis and G1790A was also investigated.ResultsA total of 5985 cases and 6809 controls in 28 case–control studies were included in this meta-analysis. The A allele of HIF-1α gene G1790A polymorphism was found to be significantly associated with increased cancer risk in four genetic models: AA + AG vs. GG (dominant model OR = 1.85, 95% CI = 1.27-2.69), AA vs. AG + GG (recessive model OR = 5.69, 95% CI = 3.87-8.37), AA vs. GG (homozygote comparison OR = 6.63, 95% CI = 4.49-9.79), and AG vs. GG (heterozygote comparison OR = 2.39, 95% CI = 1.53-3.75). This variant was also significantly associated with higher risks of pancreatic cancer, head and neck cancer, lung cancer and renal cell carcinoma. However, the A allele of G1790A was not significantly associated with increased lymph node metastasis in the dominant model by overall meta-analysis.ConclusionsOur meta-analysis suggests that the substitution of G with A of HIF-1α gene G1790A polymorphism is a risk factor of cancer, especially for pancreatic cancer, lung cancer, renal cell carcinoma and head and neck cancer. The association is significant in Asian, Caucasian population and public based control subgroups. However, it’s not associated with risk of lymph node metastasis.

Tissue microarray analysis reveals the expression and prognostic significance of phosphorylated AktThr308 in oral squamous cell carcinoma

OBJECTIVES We aimed to investigate the association between the expression of phosphorylated Akt(Thr308) (p-Akt(Thr308)) in oral squamous cell carcinoma (OSCC) tissues and clinicopathological parameters of OSCC patients and to verify the validity of p-Akt(Thr308) as a prognostic biomarker. STUDY DESIGN One hundred and ninety-one patients with OSCC were recruited for the study. We tested the expression of p-Akt(Thr308) by immunohistochemistry (IHC) with tissue microarray (TMA) and analyzed with digital pathology analysis software. The clinicopathological parameters of all patients were collected from follow-up. RESULTS P-Akt(Thr308) was detected in 95.2% of OSCC patients. The expression of p-Akt(Thr308) was significantly correlated with local recurrence and five-year survival rate. High expression of p-Akt(Thr308) in OSCC was associated with poor prognosis. CONCLUSION P-Akt(Thr308) might be a candidate biomarker for the prediction of OSCC prognosis.

The Activation of NF-κB in Infiltrated Mononuclear Cells Negatively Correlates with Treg Cell Frequency in Oral Lichen Planus.

Oral lichen planus (OLP) is a T cell-mediated chronic inflammatory mucosal disease with persistent accumulation of T cells in the lamina propria. Nuclear factor-kappa B (NF-κB) is a major regulator of immune responses, and NF-κB-dependent cytokines and pro-inflammatory mediators can be detected in higher levels in the saliva and serum from patients with OLP. CD4+CD25+Foxp3+ regulatory T (Treg) cells play an important role in the prevention of autoimmune pathology by regulating the immune response. To explore the correlation between NF-κB p65 activation and accumulation of Treg cells in patients with OLP, 40 ethnic Chinese patients with OLP and 10 healthy volunteers were recruited. The nuclear expression of NF-κB p65 in infiltrated mononuclear cells and Treg cells in the OLP lesion and the normal oral mucosa (NOM) was analyzed by immunohistochemistry assay. Our results showed that both the nuclear expression of NF-κB p65 and the number of Foxp3+ Treg were higher in the OLP lesions. Furthermore, the frequency of Treg cells was negatively correlated with NF-κB nuclear expression in subepithelial lymphocytic infiltrate of the OLP lesion. This finding provides a new insight into the pathogenesis of OLP and may contribute to novel therapeutic strategies for the treatment of OLP by modulating the immune system.

Widespread purple bulla-like masses of the oral mucosa

CLINICAL PRESENTATION A 65-year-old man complained of an inflexible tongue and painless lesions affecting his whole oral cavity for 1 year. The condition had gotten worse in the past 6 months, and his submandibular region had been swollen for 5 months. The patient denied fatigue, headaches, weight change, fever, chills, or night sweats during this period. Nor did he have any skin lesions, sinus congestion, chest pain, palpitations, abdominal pain, diarrhea, malabsorption, dysuria, stool change, or musculoskeletal pain. According to his medical history, the patient had a lumbar spine injury 1 year before, a gastric ulcer, and moderate anemia for 3 months. He drinks alcohol occasionally and has smoked 10 cigarettes per day for 30 years. He denied any history of allergy or family history of diseases. Oral examination showed numerous purple and redpurple bulla-like masses on the labial and buccal mucosa (Figure 1, A and B). Most of these lesions were very soft, but a few of them were dense on palpation. Lesions on the tongue were similar to those on the labial and buccal mucosa, and the size of the tongue was normal (Figure 1, C). However, the lesions on the ventral surface of tongue were firm on palpation. This might explain the inflexibility of the tongue. A 7.5 4 3 cm, dense on palpation, painless swelling was found in the submandibular region (Figure 1, D).

Medical treatments for pregnant patients with oral lichen planus

Abstract Oral lichen planus (OLP) is a common chronic inflammatory disorder that manifests as papular, reticular, or erosive lesions. OLP seriously affects a patient’s quality of life, as it is associated with symptoms such as pain and a burning sensation. It is also accompanied by a risk of carcinogenic tendency. During pregnancy, the treatment will be more complicated because of the effect of medical treatment on both the mother and foetus. Thus, appropriate drugs for those pregnant patients will be more essential. This study aimed to review the safety of drugs used for the treatment of OLP during pregnancy and to establish an appropriate treatment plan for pregnant patients with OLP.

Human papillomavirus-32-associated focal epithelial hyperplasia accompanying HPV-16-positive papilloma-like lesions in oral mucosa.

AbstractHuman papillomavirus infection can cause a variety of benign or malignant oral lesions, and the various genotypes can cause distinct types of lesions. To our best knowledge, there has been no report of 2 different human papillomavirus-related oral lesions in different oral sites in the same patient before. This paper reported a patient with 2 different oral lesions which were clinically and histologically in accord with focal epithelial hyperplasia and oral papilloma, respectively. Using DNA extracted from these 2 different lesions, tissue blocks were tested for presence of human papillomavirus followed by specific polymerase chain reaction testing for 6, 11, 13, 16, 18, and 32 subtypes in order to confirm the clinical diagnosis. Finally, human papillomavirus-32-positive focal epithelial hyperplasia accompanying human papillomavirus-16-positive oral papilloma-like lesions were detected in different sites of the oral mucosa. Nucleotide sequence sequencing further confirmed the results. So in our clinical work, if the simultaneous occurrences of different human papillomavirus associated lesions are suspected, the multiple biopsies from different lesions and detection of human papillomavirus genotype are needed to confirm the diagnosis.

Associations Between Three Polymorphisms in the Interleukin-4 Receptor Gene and Risk of Cancer: a Meta-analysis

Interleukin-4 receptor (IL-4R) gene single nucleotide polymorphisms (SNPs) are implicated in cancer development. However, results from the published reports have remained inconclusive. The objective of this study was to conduct a meta-analysis investigating the association between polymorphisms in IL-4R gene and cancer risk. Pubmed, EMBASE and China National Knowledge Infrastructure (CNKI) were searched for case- control studies published up to October 30, 2012 that investigated IL-4R polymorphisms and cancer risk. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate the strength of any associations. Three IL-4R polymorphisms (Q576R, rs1801275; I75V, rs1805010; S503P, rs1805015) in 21 case-control studies were analyzed. Our meta-analysis indicated that these three polymorphisms are not associated with cancer risk when all studies were pooled together. In the subgroup analysis by tumor site, the results showed that Q576R G allele carriers were associated with a significantly decreased cervical cancer risk (recessive model: OR = 0.77, 95%CI = 0.60-0.98; homozygote comparison: OR = 0.76, 95%CI = 0.58-0.98). I75V G allele carriers were associated with a decreased risk of renal cancer (dominant model = 0.71, 95%CI = 0.57-0.89, heterozygote comparison: OR = 0.69, 95%CI = 0.55-0.87). When stratified by ethnicity, Q576R G allele carriers were associated with a decreased cancer risk in Caucasians (dominant model: OR = 0.90, 95%CI = 0.83-0.98; heterozygote comparison: OR = 0.89, 95%CI = 0.82-0.98). I75V G allele carriers were associated with a decreased cancer risk in Asians (heterozygote comparison: OR = 0.76, 95%CI = 0.62-0.94). S503P C allele carriers were also associated with a decreased cancer risk in Asians (CC VS TT: OR = 0.29, 95%CI = 0.08-0.99). Our results suggest that Q576R, I75V and S503P may be associated with a decreased cancer risk for certain types of cancers and in some specific ethnic groups. Future case-control studies with large sample size are needed to evaluate these associations in detail.

Correlation between prostate stem cell antigen gene expression and oral squamous cell carcinoma

The aetiology of oral squamous cell carcinoma (OSCC) remains unclear. Numerous single nucleotide polymorphisms (SNPs) associated with cancer have been identified using genome-wide association studies (GWAS). The present study was designed to identify common SNPs associated with cancer susceptibility and to evaluate their involvement in OSCC. Susceptible loci were identified by analysing a cancer GWAS catalogue. A multicentre case-control study using an OSCC and control population was performed for selected SNPs. The function of the selected locus and its associated gene was explored using a reverse transcription-polymerase chain reaction, enzyme linked immunosorbent assay and immunohistochemistry. The association between genotypes and clinical parameters was assessed in 76 patients with OSCC. Rs2294008 located in the prostate stem cell antigen gene (PSCA) was selected. It was identified that the rs2294008 polymorphism was associated with OSCC susceptibility and PSCA may be involved in the development, progression and prognosis of OSCC.