Jim J. Miller

Molecular subtypes of Campylobacter spp., Salmonella enterica, and Escherichia coli O157:H7 isolated from faecal and surface water samples in the Oldman River watershed, Alberta, Canada

Campylobacter spp., Salmonella enterica, and Escherichia coli O157:H7 isolated from 898 faecal, 43 sewage, and 342 surface water samples from the Oldman River were characterized using bacterial subtyping methods in order to investigate potential sources of contamination of the watershed. Among these pathogens, Campylobacter spp. were the most frequently isolated from faecal, sewage, and surface water samples (266/895, 11/43, and 91/342, respectively), followed by Salmonella (67/898, 8/43, and 29/342, respectively), and E. coli O157:H7 (16/898, 2/43, and 8/342, respectively). Salmonella Rubislaw was the most common serovar isolated from water. This serovar was also isolated from two wild bird species. Most other serovars isolated from water were either not isolated from animals or were isolated from multiple species. E. coli O157:H7 was predominantly isolated from cattle. The most common phage-types of this pathogen from cattle were also the most common among water isolates, and there were exact pulsed field gel electrophoresis and comparative genomic fingerprint matches between cattle, sewage, and water isolates. Campylobacters were commonly isolated from surface waters and faeces from most animal species. Restriction fragment length polymorphism of the Campylobacter flaA gene identified several location and host species-specific (cattle, goose, pig) fingerprints. Molecular subtyping of these bacterial pathogens shows considerable promise as a tool for determining the sources of faecal pollution of water.

Earthworm populations as affected by long-term tillage practices in southern Alberta, Canada

Abstract The number of earthworms under long-term (25 years) zero tillage and conventional tillage in a dryland wheat-fallow rotation were studied. Data were collected from the fallow phase of the rotation on the same field plots in both 1992 and 1994. Earthworm abundance was determined by handsorting. The results showed that there were significantly more earthworms found under zero tillage than under conventional tillage in both 1992 and 1994. The majority of earthworms were in the 5–15 cm depth in both years. All the earthworms were identified as the endogeic species Aporrectodea caliginosa (Savigny) according to Sims and Gerard (1985). We conclude that there is a significant effect of long-term dryland tillage practices on earthworms in southern Alberta, Canada.

Inactivation of Giardia Cysts and Cryptosporidium Oocysts in Beef Feedlot Manure By Thermophilic Windrow Composting

Effects of composting manure on viability of Giardia cysts (GC) and Cryptosporidium cysts (CO) were determined in a two-year study with manure from feedlot cattle bedded on barley straw or woodchips. Each year, manure was deposited in 8 m × 2.5 m × 2 m windrows (one per bedding type) on a sheltered concrete pad. On day 0, porous bags containing 100 g of feces from confirmed Giardia- and Cryptosporidium-positive cattle (9 bags per retrieval day in Year 1; 3 per day in Year 2) were implanted in the windrows. Replicate bags were placed on the concrete pads as uncomposted controls. Windrow temperature and water content were measured and compost was turned mechanically twice each week. Fecal bags were retrieved and subsampled for enumerations of total and viable cysts and cysts after 1, 2, 3, 7, 10, 17, 24 and 31 days in 1998 and after 1, 2, 3, 7, 9, 16, 23, 30, 42, 56, 70 and 98 days in 1999. Windrow temperatures (TEMP) exceeded 55°C during the fourth week of composting, and remained above 50°C for 4 wk thereafter in 1999. Bedding material did not affect overall mean temperature in either year, but TEMP was higher with straw than with woodchip from day 10 to day 17 in 1998 (P < 0.05) and on day 16 and day 42 in 1999 (P < 0.10). In 1998, moisture content decreased from 62.2 to 43.2% during composting of woodchip manure and from 67.3 to 39.3% during composting of straw manure. In 1999, moisture content decreased from 61.0 to 31.8% for woodchip and from 64.5 to 28.6% for straw compost. Percentage of viable CO declined gradually over the 31 days in 1998. The following year revealed a rapid decline in viability of GC and CO once compost temperature exceeded 55°C and viabilities of GC and CO were reduced to zero after 42 days (straw compost) and after 56 days (woodchip compost). Exposure of CO and GC to temperatures > 55°C for a period of 15 days appears to be an effective method of inactivating Giardia cysts and Cryptosporidium cysts in feedlot manure.

Spatial and temporal drivers of zoonotic pathogen contamination of an agricultural watershed.

In regions where animal agriculture is prominent, such as southern Alberta, higher rates of gastrointestinal illness have been reported when compared with nonagricultural regions. This difference in the rate of illness is thought to be a result of increased zoonotic pathogen exposure through environmental sources such as water. In this study, temporal and spatial factors associated with bacterial pathogen contamination of the Oldman River, which transverses this region, were analyzed using classification and regression tree analysis. Significantly higher levels of fecal indicators; more frequent isolations of Campylobacter spp., Escherichia coli O157:H7, and Salmonella enterica spp.; and higher rates of detection of pig-specific Bacteroides markers occurred at downstream sites than at upstream sites, suggesting additive stream inputs. Fecal indicator densities were also significantly higher when any one of these three bacterial pathogens was present and where there were higher total animal manure units; however, occasionally pathogens were present when fecal indicator levels were low or undetectable. Overall, Salmonella spp., Campylobacter spp., and E. coli O157:H7 presence was associated with season, animal manure units, and total rainfall on the day of sampling and 3 d in advance of sampling. Several of the environmental variables analyzed in this study appear to influence pathogen prevalence and therefore may be useful in predicting water quality and safety and in the improvement of watershed management practices in this and other agricultural regions.

Influence of Streambank Fencing on the Environmental Quality of Cattle-Excluded Pastures

Limited information exists on the effect of streambank fencing on riparian zone pastures. The objective of this study was to test the hypothesis that 4 to 6 yr of streambank fencing would improve the environmental quality of the cattle-excluded pasture compared with the grazed pasture and cause the fenced pasture to act as a buffer or filter strip. Rangeland health, vegetative and soil properties, and rainfall simulation runoff were measured in the cattle-excluded and adjacent grazed native pastures along the fenced reach of the Lower Little Bow River in southern Alberta, Canada, for 3 yr (2005-2007). Rangeland health was improved (health score increase from 55 to 72%); vegetation cover (13-21%) and standing litter (38-742%) were increased; and bare soil (72-93%) and soil bulk density (6-8%) were decreased under cattle exclusion, indicating an improvement in environmental quality from streambank fencing. In contrast, other vegetation (total and live basal area, fallen litter) and soil properties (soil water and soil C, N, and P) were not improved by cattle exclusion. Cattle exclusion significantly (P </= 0.10) reduced surface runoff depth of water (21-32%) and mass loads of total N fractions (21-52%) in 2 of 3 yr compared with the grazed pasture, suggesting that this fenced pasture may act as a buffer for certain runoff variables. In contrast, other runoff variables (turbidity, electrical conductivity, pH, concentrations and loads of total suspended solids, and certain N and P fractions) in the cattle-excluded pasture were generally not improved by streambank fencing. Overall, streambank fencing improved the quality of certain environmental variables within the cattle-excluded pasture.

Barley dry matter yield, crop uptake, and soil nutrients under fresh and composted manure containing straw or wood-chip bedding

Limited information exists on the effect of fresh versus composted beef cattle manure containing straw or wood chips on barley (Hordeum vulgare) yield, nutrient uptake, and soil nutrient status in the Great Plains region of North America. Barley was grown on an irrigated clay loam soil in southern Alberta from 1999 to 2001. The treatments were three rates (13, 39, 77 Mg dry material per hectare) of fresh manure (FM) or composted manure (CM) containing either straw (ST) or wood-chip (WD) bedding, one inorganic (IN) fertilizer treatment (100 kg N ha-1, 17 kg P ha-1), and a control treatment; applied in the fall of 1998, 1999, and 2000. Dry matter yield was not significantly (P > 0.05) influenced by manure type or bedding material. Crop protein was 7% higher under FM (12.7 g kg-1) than CM (11.9 g kg-1) in 2001, and crop N uptake was 11 to 13% higher for CM-ST (171.3 kg ha-1) and FM-WD (174.9 kg ha-1) than CM-WD (154.7 kg ha-1) over the 3 yr. Soil available N was 20 to 261% higher for FM than CM at the 39 and...

Available nitrogen and phosphorus in soil amended with fresh or composted cattle manure containing straw or wood-chip bedding

Limited research exists on the effect of fresh versus composted beef cattle manure containing straw or wood chips on available N and P in the Great Plains region of North America. Barley was grown (1999-2007) on an irrigated clay loam soil in southern Alberta where organic amendments and fertilizer were applied annually for 9 yr from 1998 to 2006. The treatments were three rates (13, 39, 77 Mg dry wt. ha-1) of fresh manure (FM, stockpiled for up to 2 m) or composted manure (CM) containing either straw (ST) or wood chip (WD) bedding, one inorganic (IN) fertilizer treatment, and a unamended control. The soil was sampled in the fall of 1999 to 2002, 2004, 2006, and 2007 and was analyzed for soil inorganic N (SIN), NO3-N, NH4-N, and soil test P (STP). Soil mineralizable N was also determined on surface soil samples collected from 1999, 2002, 2004, and 2007. Manure type had a significant effect on SIN, NO3-N, STP, and soil mineralizable N, but the effects varied with year or bedding and rate (SIN), rate or yea...

Soil salinity and sodicity after application of fresh and composted manure with straw or wood-chips

Limited information exists on the effect of fresh versus composted beef cattle manure containing straw or wood chips on salinity (EC), soluble salts (Ca, Mg, Na, K, Cl, SO4), sodicity (sodium adsorption ratio, SAR), and potassium adsorption ratio (PAR) status of soil in the Great Plains region of North America. A site on a clay loam soil under irrigated barley (Hordeum vulgare) in southern Alberta was used for this study. The treatments were three rates (13, 39, 77 Mg ha-1 dry material) of fresh manure (FM) or composted manure (CM) containing either straw (ST) or wood-chip (WD) bedding, one inorganic (IN) fertilizer treatment, and a control (CON) treatment. All treatments were applied in the fall of 1998, 1999, and 2000. Soil sampling was conducted in the fall of 1998, 1999, 2000, and 2001 prior to manure and fertilizer application. Our results indicated that applying CM to irrigated barley for 3 yr at rates up to 77 Mg ha-1 should not cause an increase in any of the salinity variables compared with FM. C...

A methods comparison for the isolation and detection of thermophilic Campylobacter in agricultural watersheds

Campylobacter species contribute to an enormous burden of enteric illnesses around the world. This study compared two different methods for detecting Campylobacter species in surface water samples from agricultural watersheds across Canada. One method was based on membrane filtration (MF) of 500 ml water samples followed by selective microaerophilic enrichment at 42 degrees C in Bolton broth, isolation of Campylobacter on CCDA, and subsequent identification confirmation by a PCR assay. The second method was based on centrifugation (CF) of 1000 ml water samples, followed by selective microaerophilic enrichment at 42 degrees C in Bolton broth, isolation of Campylobacter on Modified Karmali Agar, and subsequent identification confirmation by a different PCR assay. Overall comparison of the CF and MF methods indicated that both methods found Camylobacterjejuni to be the most commonly detected Campylobacter species in 699 water samples from four agricultural watersheds across Canada, and that C. jejuni frequency of occurrence was similar by both methods. However, the CF method detected significantly higher frequencies of Campylobactercoli (17%) and other Campylobacter species (13%) than the MF method (11% and 3%, respectively). It was frequently found that one method would detect Campylobacter in a water sample when the other method would not for a simultaneously collected, duplicate water sample. This study indicates that methods can have significantly different recovery efficiencies for Campylobacter species, and that caution is needed when comparing studies that report on the frequency of occurrence of waterborne Campylobacter at the genus level when different detection methods are used.

UNSATURATED HYDRAULIC CONDUCTIVITY OF CONVENTIONAL AND CONSERVATION TILLAGE SOILS IN SOUTHERN ALBERTA

Conservation tillage is increasing on the Canadian prairies and its long-term effect on soil physical properties warrants investigation. Tension infiltrometer measurements were conducted on conventional tillage (CT), minimum tillage (MT) and no-till (NT) loam to clay loam soils in southern Alberta to determine if 26 yr of conservation tillage (MT, NT) modified the unsaturated hydraulic conductivity, K(ψ), relative to CT. Tillage of CT and MT plots was performed using a wide-blade cultivator. Measurements were performed on tillage treatments that were replicated on two adjacent parcels of land, with readings taken at the west parcel (Site 1) in 1993 and at the east parcel (Site 2) in 1994. Infiltration rates were determined at water potentials (ψ) of −0.3, −0.6 and −1.5 kPa. The K(ψ) values at −0.3, −0.6, −1.0 and −1.5 kPa (equivalent circular pore diameters of 1000, 500, 300 and 200 µm, respectively) were estimated from infiltration data using the nonlinear regression method of Logsdon and Jaynes. Tillage...