Jindra Vrzalova
Charles University in Prague
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Featured researches published by Jindra Vrzalova.
Transfusion | 2011
Daniel Lysák; Marcela Hrabětová; Jindra Vrzalova; Vladimír Koza; Jana Navrátilová; Tomáš Svoboda; Alexandra Jungová; Ondřej Topolčan
BACKGROUND: Peripheral blood stem cells are an important source of hematopoietic stem cells (HSCs) for allogeneic transplantations. Some allogeneic donors mobilize HSCs poorly in response to the granulocyte—colony‐stimulating factor (G‐CSF). The estimation of the mobilization result in an individual donor is difficult due to the absence of suitable predictive factors.
PLOS ONE | 2014
Lenka Belinova; Hana Kahleova; Hana Malinska; Ondrej Topolcan; Jindra Vrzalova; Olena Oliyarnyk; Ludmila Kazdova; Martin Hill; Terezie Pelikanova
Background The intake of meat, particularly processed meat, is a dietary risk factor for diabetes. Meat intake impairs insulin sensitivity and leads to increased oxidative stress. However, its effect on postprandial gastrointestinal hormone (GIH) secretion is unclear. We aimed to investigate the acute effects of two standardized isocaloric meals: a processed hamburger meat meal rich in protein and saturated fat (M-meal) and a vegan meal rich in carbohydrates (V-meal). We hypothesized that the meat meal would lead to abnormal postprandial increases in plasma lipids and oxidative stress markers and impaired GIH responses. Methods In a randomized crossover study, 50 patients suffering from type 2 diabetes (T2D) and 50 healthy subjects underwent two 3-h meal tolerance tests. For statistical analyses, repeated-measures ANOVA was performed. Results The M-meal resulted in a higher postprandial increase in lipids in both groups (p<0.001) and persistent postprandial hyperinsulinemia in patients with diabetes (p<0.001). The plasma glucose levels were significantly higher after the V-meal only at the peak level. The plasma concentrations of glucose-dependent insulinotropic peptide (GIP), peptide tyrosine-tyrosine (PYY) and pancreatic polypeptide (PP) were higher (p<0.05, p<0.001, p<0.001, respectively) and the ghrelin concentration was lower (p<0.001) after the M-meal in healthy subjects. In contrast, the concentrations of GIP, PYY and PP were significantly lower after the M-meal in T2D patients (p<0.001). Compared with the V-meal, the M-meal was associated with a larger increase in lipoperoxidation in T2D patients (p<0.05). Conclusion/Interpretation Our results suggest that the diet composition and the energy content, rather than the carbohydrate count, should be important considerations for dietary management and demonstrate that processed meat consumption is accompanied by impaired GIH responses and increased oxidative stress marker levels in diabetic patients. Trial Registration ClinicalTrials.gov NCT01572402
Pathobiology | 2013
Lada Eberlová; Zbyněk Tonar; Kirsti Witter; Věra Křížková; Lukáš Nedorost; Marie Korabecna; Pavel Tolinger; Kocová J; Ludmila Boudova; Vladislav Třeška; Karel Houdek; Jiří Moláček; Jindra Vrzalova; Martin Pesta; Ondřej Topolčan; Jiří Valenta
Objective: Abdominal aortic aneurysm (AAA) is a serious disease due to its covert nature, relatively high prevalence and fatal prognosis in the case of rupture. To obtain new insights into AAA pathogenesis, we examined the relationships between histopathology, multiplex in vitro immunoassay data, diameter and symptomatology. Methods: In a prospective, non-randomised study, we evaluated samples from 6 normal infrarenal aortae and 65 AAA patients (65 walls, 55 thrombi). The AAA patients were either asymptomatic (n = 44), symptomatic (n = 7) or with ruptured AAA (n = 14). The AAA diameter was classified as small (<5 cm, n = 18), medium (5–7 cm, n = 26) and large (>7 cm, n = 21). We quantified the histopathology of the AAA wall and the adjacent thrombus. We assessed the expression of proteins in the same samples. Results: Asymptomatic AAAs had walls with more abundant inflammatory infiltrates, lower amounts of PAI-1, a higher number of tPA-positive elements, a tendency towards decreased collagen content, whereas the adjacent thrombi had a greater concentration of VCAM-1 and MMP-2 when compared with symptomatic AAAs. Compared with the aneurysmatic aorta, the normal aorta contained less collagen and more elastin, actin, desmin and PAI-1-positive elements; in addition, it was more vascular. Medium-sized AAAs were the most actin and vimentin rich, and large AAAs were the most vascular. Conclusion: Our results show that asymptomatic AAA walls often have more potentially deleterious histopathological alterations than symptomatic AAA walls. This result indicates that a progression from an asymptomatic AAA to rupture can be expected and screening patients who are at risk of rupture could be beneficial.
Journal of Ophthalmology | 2015
Stepan Rusnak; Jindra Vrzalova; Marketa Sobotova; Lenka Hecova; Renata Ricarova; Ondrej Topolcan
Purpose. To determine the intraocular levels of growth factors and cytokines in patients with various degrees of severity of proliferative diabetic retinopathy (PDR) using multiplex xMAP technology. Methods. A prospective cohort study of 61 eyes from 56 patients who were divided into 3 groups based on the severity of PDR. Patients in group number 1 are those who presented PDR with no need of repeated surgical intervention; patients in group number 2 had repeated vitreous bleeding; and patients in group number 3 had refractory neovascular glaucoma. The concentrations of proangiogenic, antiangiogenic, inflammatory, and neurotrophic factors were measured in intraocular fluid. The results were also compared with levels of factors measured in 50 eyes from 50 patients prior to senile cataract surgery (control group). Results. Patients with refractory neovascular glaucoma (the highest clinical severity group) had higher levels of interleukin 6 (IL-6) (median1 37.19; median3 384.74; P = .00096), transforming growth factor beta 1 (TGFβ-1) (median1 49.00; median3 414.40; P = .0017), and vascular endothelial growth factor (VEGF) (median1 211.62; median3 352.82; P = .0454) compared with other PDR patients. Conclusions. Results of our study imply that levels of IL-6, TGFβ-1, and VEGF correlate with the severity of PDR.
American Journal of Reproductive Immunology | 2009
Zdenka Ulcova-Gallova; Jitka Gruberova; Jindra Vrzalova; Katarina Bibkova; Jana Peknicova; Zdenka Micanova; Ondrej Topolcan
Problem The aim of this study was to investigate seminal sperm‐agglutinating antibodies, intra‐acrosomal proteins, sperm head abnormalities, and cytokines (IL‐1β, IL‐2, IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, IL‐12p70 TNF‐α, and IFN‐γ) in men from infertile couples.
American Journal of Reproductive Immunology | 2009
Zdenka Ulcova-Gallova; Jitka Gruberova; Jindra Vrzalova; Katarina Bibkova; Jana Peknicova; Zdenka Micanova; Ondrej Topolcan
Problem The aim of this study was to investigate seminal sperm‐agglutinating antibodies, intra‐acrosomal proteins, sperm head abnormalities, and cytokines (IL‐1β, IL‐2, IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, IL‐12p70 TNF‐α, and IFN‐γ) in men from infertile couples.
PLOS ONE | 2014
Hana Malinska; Hana Kahleova; Ondrej Topolcan; Jindra Vrzalova; Olena Oliyarnyk; Ludmila Kazdova; Lenka Belinova; Martin Hill; Terezie Pelikanova
Background Abnormal postprandial elevation of plasma glucose and lipids plays an important role in the pathogenesis of diabetes and strongly predicts cardiovascular mortality. In patients suffering from type 2 diabetes (T2D) postprandial state is associated with oxidative stress, cardiovascular risk and, probably, with impairment of both secretion and the effect of gastrointestinal peptides. Evaluating postprandial changes of gastrointestinal hormones together with changes in oxidative stress markers may help to understand the mechanisms behind the postprandial state in diabetes as well as suggest new preventive and therapeutical strategies. Methods A standard meal test has been used for monitoring the postprandial concentrations of gastrointestinal hormones and oxidative stress markers in patients with T2D (n = 50) compared to healthy controls (n = 50). Blood samples were drawn 0, 30, 60, 120 and 180 minutes after the standard meal. Results Both basal and postprandial plasma concentrations of glucose and insulin proved to be significantly higher in patients with T2D, whereas plasma concentrations of ghrelin showed significantly lower values during the whole meal test. In comparison with healthy controls, both basal and postprandial concentrations of almost all other gastrointestinal hormones and lipoperoxidation were significantly increased while ascorbic acid, reduced glutathione and superoxide dismutase activity were decreased in patients with T2D. A positive relationship was found between changes in GIP and those of glucose and immunoreactive insulin in diabetic patients (p<0.001 and p<0.001, respectively) and between changes in PYY and those of glucose (p<0.01). There was a positive correlation between changes in GIP and PYY and changes in ascorbic acid in patients with T2D (p<0.05 and p<0.001, respectively). Conclusion/Interpretation Apart from a positive relationship of postprandial changes in GIP and PYY with changes in ascorbic acid, there was no direct link observed between gastrointestinal hormones and oxidative stress markers in diabetic patients. Trial Registration ClinicalTrials.gov NCT01572402
Biomarkers in Medicine | 2013
Stepan Rusnak; Jindra Vrzalova; Lenka Hecova; Marketa Kozova; Ondrej Topolcan; Renata Ricarova
AIM Proliferative vitreoretinopathy is the major cause of retinal detachment surgery failure. Our prospective cohort study of 27 eyes aimed to determine intraocular levels of growth factors and cytokines in patients with retinal detachment with various degrees of severity of proliferative vitreoretinopathy using multiplex xMAP(®) Technology. PATIENTS & METHODS The concentrations of 12 proangiogenic, antiangiogenic, inflammatory and neurotrophic factors were measured from 0.05-ml samples of intraocular fluid using multiplex xMAP Technology. The results were compared with levels of various factors, which were measured in samples from the control group of 31 eyes prior to senile cataract surgery. RESULTS The concentration of the MCP-1 cytokine was found to be higher in eyes with retinal detachment compared with the control group. The concentration of VEGF was found to be higher in eyes with retinal detachment complicated with proliferative vitreoretinopathy compared with the uncomplicated retinal detachment group and the control group. CONCLUSION MCP-1 and VEGF may participate in pathogenesis of retinal detachment and proliferative vitreoretinopathy. Biomarkers in disease detection and management have become important tools in modern clinical medicine, and their application to retinal disease should be no exception.
American Journal of Reproductive Immunology | 2009
Zdenka Ulcova-Gallova; Jitka Gruberova; Jindra Vrzalova; Katarina Bibkova; Jana Peknicova; Zdenka Micanova; Ondrej Topolcan
Problem The aim of this study was to investigate seminal sperm‐agglutinating antibodies, intra‐acrosomal proteins, sperm head abnormalities, and cytokines (IL‐1β, IL‐2, IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, IL‐12p70 TNF‐α, and IFN‐γ) in men from infertile couples.
The Epma Journal | 2014
Radka Fuchsova; Ondrej Topolcan; Jindra Vrzalova; Milan Hora; Radek Kucera; Olga Dolejšová; Ondrej Hes; Jiri Ferda
Technological approaches The Immunoanalytical Laboratory of University Hospital in Pilsen examined sera of 76 patients from the Urology department of the University Hospital with suspected prostate cancer who have undergone TRUS biopsy. We assessed the levels of PSA and, if the interval of PSA was between 0-30 ng/mL, we also assessed the levels of freePSA, [-2]proPSA and we calculated %freePSA and Prostate Health Index (PHI). The monitored biomarkers were measured using the chemiluminescent DxI 800 instrument (Beckman Coulter, USA). The peripheral blood was drawn by VACUETTE ® (Greiner Bio-One, Austria). All specimens were immediately aliquoted, frozen and stored at -80°C. Samples were thaw only ones just before the processing. SAS 9.2 software was used for all statistical analysis.