Jiří Klinot

Use of cyclodextrins in isotachophoresis : VI. Cyclodextrins as leading electrolyte additives for the separation of bile acids

Cyclodextrins (CDs) and some of their methyl derivatives have been used for the optimization of the isotachophoretic separation of bile acids in aqueous electrolyte systems. The addition of heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin to the leading electrolyte proved useful for both the solubilization and the structural differentiation of the solutes studied and led to the successful separation of their mixtures. Other CDs tested, even if they gave a satisfactory solubilization effect, did not support the resolution of bile acid mixtures.

Classical bile acids in animals, β-phocaecholic acid in ducks

Abstract 1. 1. Bile samples of different animals were analysed and the percentage content of classical bile acids was determined. 2. 2. Herbivorous birds mostly excreted a large proportion of chenodeoxycholic acid. 3. 3. The anteater (Myrmecophaga tridactyla) excreted deoxycholic acid most probably as a primary bile acid. 4. 4. In the bile of ducks (Anas platyrhynchos) a large amount of (23R)3α,7α,23-trihydroxy-5β-cholan-24-oic acid (β-phocaecholic acid) was found.