Jiřina Pospíšilová

DETERMINATION OF GENTIOPICROSIDE IN EXTRACTS OF CENTAURIUM ERYTHREAE AND GENTIANA LUTEA BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Micellar electrokinetic capillary chromatography has been developed as a promising method for the determination of gentiopicroside in plant samples. The separation conditions have been optimised with respect to the different parameters including SDS concentration, pH, and ion strength of the background electrolyte, and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.6 was found to be the most suitable electrolyte for this separation. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 20°C gave the best analysis of gentiopicroside. The linear detection range for concentration versus peak area for the assay is from 5 to 500 μg. mL−1 (correlation coefficient 0.9998) with a detection limit of 0.3 μg . mL−1. The inter-day reproducibility of the peak area was below 2.5% and the inter-day reproducibility of the migration time was below 0.16 %.

Humoral response in patients with chronic heart failure

AIM Correlation of five humoral markers with laboratory, echocardiographic and right heart catheterization parameters in patients with chronic heart failure. STUDY POPULATION 29 patients, heart failure NYHA II and III, ejection fraction below 40% with coronary artery disease or dilated cardiomyopathy. METHODS evaluation of thromboxane, prostaglandin F (PGF), tumor necrosis factor (TNF) alpha, endothelin-1 and big endothelin rest levels and their correlation with: (1) laboratory parameters: Sodium, urea, creatinine, fibrinogen, (2) chest X-ray: cardiothoracic index (CTI), pulmonary congestion, (3) right heart catheterization parameters at rest, hand-grip and bicycle ergometry: mean pulmonary artery pressure (AP), wedge pressure (WP), systemic and pulmonary vascular resistance (SVR, PVR) and cardiac index (CI), (4) echocardiographic parameters at rest, hand-grip and bicycle ergometry: end-diastolic volume (EDV), end-systolic volume (ESV), ejection fraction (EF), mitral flow E/A, filling period of left ventricle and time of duration of mitral regurgitation. RESULTS No correlation was found between thromboxane, prostaglandin F and tumor necrosis factor alpha with the above mentioned parameters. Endothelin-1 level correlated with E/A, PVR and MPA at rest and at hand-grip. Big endothelin level correlated with EDV and ESV, AP, WP and SVR at rest and at both types of exercise. The highest correlation was between big endothelin and rest AP (r=0.79), rest WP (r=0.78) and CTI (r=0.58), all P<0.01. CONCLUSIONS Big endothelin and partly endothelin-1 levels showed a close correlation with some parameters used for the evaluation of chronic heart failure severity.