Jiro Suzuki

The Production of Flocculating Substance(s) by Streptomyces griseus

Streptomyces griseus produced a certain kind of flocculating substance(s). The flocculating substance(s) could aggregate suspended kaolin particles in solution to form small floes and enables it to sediment those particles. The production of flocculating substance(s) by S. griseus was not parallel to the cell growth and a large number of flocculating substance(s) was released into the culture broth in the death phase. The flocculating substance(s) produced by S. griseus acted under acidic conditions and the maximum activity was observed at pH 4.

Ultrastructure of the acidophilic aerobic photosynthetic bacterium Acidiphilium rubrum.

Abstract. The ultrastructure of cells of Acidiphilium rubrum, which is an acidophilic aerobic photosynthetic bacterium containing zinc-complexed bacteriochlorophyll a, was studied by electron microscopy with the rapid substitution technique. Thin-section electron microscopy indicated that any type of internal photosynthetic membranes was not present in this organism despite a relatively high content of the photopigment. The majority of cells had poly-β-hydroxybutyrate granules and electron-dense spherical bodies identified as being polyphosphate granules. When the organism was grown chemotrophically with 0.1% FeSO4, it produced another group of electron-dense granules that were associated with the inner part of the cytoplasmic membrane. An energy-dispersive X-ray analysis showed that these membrane-bound, electron-dense granules contained iron.

Synthesis of an Affinity Adsorbent for Sialidase

Abstract The reaction of the sodium salt of methyl 5-acetamido-4,7,8,9-tetra-O-acetyl-3,5-dideoxy-2-thio-D-glycero-α-D-galacro-2-nonulopyranosonate (1) with styrene oxide, followed by deprotection, gave the diastereomeric mixture of (β-hydroxyphenethyl) 5-acetamido-3,5-dideoxy-2-thio-D-glycero-α-D-galycto-2-nonulopyranosidoic acid (3). The thioglycoside 3 was proved to be an inhibitor for Clostridium perfringens sialidase. An affinity adsorbent with immobilized sialic acid through a thioglycosidic linkage was prepared by treatment of 1 with epoxy-activated Sepharose 4B. The synthetic adsorbent was proved to adsorb Cl. perfringens sialidase specifically and to be capable of repeated operation.