Jl Crawford

Lacunocanalicular Flow in Osseous Repair and Regeneration

INTRODUCTION: Our hypothesis is that the tension stress of activation increases lacunocanalicular flow and upregulates the mechanotransductive-osteogenic pathway. Furthermore, we hypothesize improving vascularization by endothelial progenitor cell (EPC) mobilization enhances lacunocanalicular flow in the consolidation period, maintaining upregulation of the mechanotransductive pathway to accelerate osteogenesis. We develop strategies to manipulate this mechanism to recapitulate lacunocanalicular flow and optimize the biomimicry of tissueengineered implants.

Alloderm as a Topical Gene Therapy Matrix To Improve Graft Vascularity

INTRODUCTION: Alloderm acellular dermal matrix is widely used for structural or dermal replacement purposes. Given its highly biocompatible quality, its infection resistance, and its potential to vascularize, we explored the possibility of Alloderm to function as a siRNA delivery matrix. Specifically, we sought to improve Alloderm vascularization by siRNA mediated inhibition of Prolyl hydroxylase domain-2 (PHD2), a cytoplasmic protein that regulates the HIF-1α pathway of angiogenic factor upregulation and neovascularization.

Chemical Delay of Flaps through Endogenous Stem Cell Therapy

INTRODUCTION: Alloderm acellular dermal matrix is widely used for structural or dermal replacement purposes. Given its highly biocompatible quality, its infection resistance, and its potential to vascularize, we explored the possibility of Alloderm to function as a siRNA delivery matrix. Specifically, we sought to improve Alloderm vascularization by siRNA mediated inhibition of Prolyl hydroxylase domain-2 (PHD2), a cytoplasmic protein that regulates the HIF-1α pathway of angiogenic factor upregulation and neovascularization.