Joachim Daum
Schering AG
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Publication
Featured researches published by Joachim Daum.
Journal of Biotechnology | 1997
Andre Koltermann; Werner Boidol; Joachim Daum; Peter Scholz; Peter Donner
An Escherichia coli K 12 strain has been constructed for efficient expression of recombinant biologically active human IL-8 (Interleukin-8). The development of a fermentation and purification process from the laboratory scale (cells from 15 l fermentation broth) to a production scale (cells from 200 l fermentation broth) is described. Material obtained from the laboratory scale was used for initial in vitro studies and for the development of a biological assay. An upscale purification process starting from 80 l fermentation broth resulted in larger amounts of IL-8 needed for preclinical studies. This process includes a fully automated control of the initial affinity chromatography step. Finally, a production process which differed markedly from the small-scale processes was tailor-made for GMP conformity and economic considerations. It consists of a cell disruption step followed by two crossflow diafiltrations with different molecular weight cut offs and filtration rates, one cation exchange chromatography and a final dialysis step. In order to enhance the overall yield of biologically active IL-8, conditions for a resolubilisation of insoluble IL-8 present in the remaining pellet after cell disruption were worked out.
Human Reproduction | 1994
Kristof Chwalisz; Marion Benson; Peter Scholz; Joachim Daum; Henning M. Beier; Christa Hegele-Hartung
Archive | 1982
Joachim Daum; Gerhard Siewert; Michael Dr. Töpert; Hartmut Seliger
Archive | 1980
Gerhard Siewert; Werner Boidol; Joachim Daum
Archive | 1980
Joachim Daum; Gerhard Siewert; Michael Dr. Töpert; Hartmut Seliger
Thrombosis and Haemostasis | 1997
Erika Glusa; Ellen Bretschneider; Joachim Daum; Christiane Noeske-Jungblut
FEBS Journal | 1989
Joachim Daum; Peter Donner; Wilhelm Geilen; Gisela Hübner‐Kosney; Marina Isernhagen; Harald Scheidecker; Hartmut Seliger; Werner Boidol; Gerhard Siewert
Archive | 1980
Gerhard Siewert; Werner Boidol; Joachim Daum
Archive | 1988
Harald Scheidecker; Joachim Daum; Peter Donner; Gerhard Siewert
Archive | 1980
Joachim Daum; Gerhard Siewert; Michael Dr. Töpert; Hartmut Seliger