Joan M. Eckerson

Effect of two and five days of creatine loading on anaerobic working capacity in women.

Law, YLL, Ong, WS, GillianYap, TL, Lim, SCJ, and Chia, EV. Effects of two and five days of creatine loading on muscular strength and anaerobic power in trained athletes. J Strength Cond Res 23(3): 906-914, 2009-The purpose of this study was to establish the effects of 2 and 5 days of creatine loading, coupled with resistance training, on muscular strength and anaerobic performance in trained athletes. Seventeen trained men were randomly assigned to a creatine or a placebo group. The creatine supplementation group consumed 20 g of creatine per day (4 doses of 5 g per day), whereas the placebo group was given a placebo similar in appearance and taste over the 5-day supplementation duration. Anaerobic power and strength performance measures, in addition to blood and urine analysis, were conducted in the morning before the supplementation began and on the third and sixth day to establish the effect of 2 and 5 days of creatine loading, respectively. The study found that a 5-day creatine loading regime coupled with resistance training resulted in significant improvements in both average anaerobic power, as measured by the 30-second Wingate test and back squat strength compared with just training alone. However, 2 days of supplementation was not sufficient to produce similar performance gains as that observed at the end of 5 days of loading in trained men, despite increases in creatine uptake in the body. The standard 5-day loading regime should hence be prescribed to individuals supplementing with creatine for enhanced strength and power.

Effect of Creatine Phosphate Supplementation on Anaerobic Working Capacity and Body Weight After Two and Six Days of Loading in Men and Women

The purpose of this study was to determine the effects of 2 and 6 days of creatine phosphate loading on anaerobic working capacity (AWC) and body weight (BW) in men and women. Sixty-one men (n = 31) and women (n = 30) randomly received 1 of 3 treatments (4 3 5 g·d-1 X 6 days) using a double blind design: (a) 18 g dextrose as placebo (PL); (b) 5.0 g Cr + 20 g dextrose (Cr); or (c) 5.0 g Cr + 18 g dextrose + 4 g of sodium and potassium phosphates (CrP). AWC was determined at baseline and following 2 and 6 days of supplementation using the Critical Power Test. BW increased significantly over time, and the mean value for the men was significantly greater compared to that for women, but there were no interactions (p > 0.05). There were gender-specific responses for AWC expressed in both absolute values (kJ) and relative to BW (kJ· kg-1), with the women demonstrating no significant interactions. For the men, CrP loading significantly increased AWC following 2 days (23.8%) and 6 days (49.8%) of supplementation vs. PL (kJ and kJ·kg-1). Cr supplementation increased AWC 13–15% in both genders compared to PL (1.1%–3.0% decline); although this result was not statistically significant, it may have some practical significance.

Fluid Tolerance while Running : Effect of Repeated Trials

This study assessed tolerance to fluid ingestion with repeated sessions of drinking while running. Runners (n = 7; age 22 +/- 2 yr; V O (2max) = 54.4 +/- 7.1 ml/kg/min) performed six 90-min runs (65 % V O (2max); separated by 7 - 11 days). During run 1, subjects drank a glucose-electrolyte solution AD LIBITUM for 1 min every 10 min. During runs 2 - 6, subjects drank a volume of the solution every 10 min equal to their sweat production over 10 min during run 1. Stomach comfort (1 - 4 scale) and gastrointestinal symptoms were also assessed every 10 min. Gastric emptying rate was determined in runs 2 and 6. Subjects consumed more (p < 0.05) fluid during runs 2 - 6 (mean +/- SD; 1247 +/- 162 ml), than during run 1 (508 +/- 476 ml). Stomach comfort improved (p < 0.05) on runs 5 and 6 (1.7 +/- 0.5 mean ranks) compared to run 2 (2.3 +/- 0.5 mean ranks). Gastric emptying rate was not different between runs 2 and 6 (12.0 +/- 1.9 ml/min vs. 12.3 +/- 2.3 ml/min, respectively). These results indicate repeated sessions of drinking at a rate matching sweat rate improves stomach comfort, however, gastric emptying rate does not change under such conditions.

Validity of bioelectrical impedance equations for estimating fat-free weight in lean males

The present study examined the validity of bioelectrical impedance (BIA) equations for estimating fat-free weight (FFW) in lean males (X +/- SD = 9.1 +/- 2.2% fat) by comparing the estimates with values obtained from underwater weighing. Sixty-eight Caucasian male volunteers served as subjects. Cross-validation analyses included examination of the constant error (CE), standard error of the estimate (SEE), r, and total error (TE). The results indicated that the equations of Oppliger et al. (16), which resulted in small TE (1.70 kg) and CE (-0.02 kg) values, most accurately estimated FFW. Simple linear regression showed that FFW was more highly correlated with body weight (BW) (r = 0.98, P < 0.0001) and resulted in a lower SEE (1.68 kg) than either height2/resistance (Ht2/R) (r = 0.81, P < 0.0001; SEE = 5.12 kg) or the independent variable (weight x resistance)/height2 [WR/Ht2] utilized by the manufacturer of the BIA analyzer (r = 0.15, P > 0.05; SEE = 8.59 kg). Multiple regression showed that when WR/Ht2, Ht2/R, resistance, body mass index, Ht2, and/or Ht was added to the prediction equation, which utilized BW alone, they accounted for less than 1% additional variance and reduced the SEE by < or = 0.16 kg. The results indicated that BW alone estimated FFW as accurately as any of the BIA equations in lean males.

The Effect of Fluid Replacement on Endurance Performance

The purpose of this study was to examine the effects of fluid replacement on power output (PO), rating of perceived exertion (RPE), heart rate (HR), body weight (BW), urine osmolarity (Uosm), and urine electrolyte concentrations ([UNa+], [UK+], [UCl-]) in physically active men (n = 4) and women (n = 7). The participants were asked to generate their highest possible PO during 60 minutes of cycling under 3 randomized conditions: ingestion of (a) no fluid (trial 1); (b) 1200 ml of distilled water (trial 2); and (c) 1200 ml of Gatorade (trial 3). BW and urine volume (Vu) were measured before and after the ride to determine sweat rate [(SR = ΔBW + Vfluid intake + Vu)/time]. The results indicated that there were no significant differences between trials for PO (123–127 W), RPE (14), HR (140–142 b·min−1), and SR (11.9–12.4 ml·min−1). However, [UNa+] was significantly (p < 0.05) lower postexercise for all 3 trials, and [UCl-] was significantly reduced following trials 2 and 3. There was a significant increase (p < 0.001) in BW postexercise for trials 2 and 3 when compared with the no-fluid trial; however, the effects of water and Gatorade were similar. These results suggest that fluid replacement during 1 hour of moderately intense cycling does not enhance performance in physically active men and women who are normally hydrated.

The Effects of Creatine Supplementation on Anaerobic Working Capacity

Anaerobic working capacity (AWC) estimated from the critical power test provides a theoretically and experimentally valid estimate of work capacity associated with muscle energy reserves adenosine triphosphate and phosphocreatine. Creatine monohydrate (CM) supplementation has been shown to increase phosphocreatine stores in skeletal muscle and, in theory, should increase AWC. Therefore, the purpose of this study was to examine the effects of supplementation with CM, CM plus carbohydrate (CHO), or CHO alone on AWC. Using a double-blind random design, 26 young men (mean age 6 SD, 19.9 6 1.6 years) were assigned to 1 of 3 treatment conditions: (a) 35 g of flavored CHO powder as a placebo (PL, n 5 8); (b) 5.25 g of CM and 1 g of CHO in a flavored powder blend (CM, n 5 9); and (c) 5.25 g of CM and 33 g of CHO in flavored powder blend (CM-CHO, n 5 9). The subjects completed 3 phases of testing on an electronically braked cycle ergometer: (a) familiarization (3 learning trials to establish power outputs for subsequent testing); (b) pretesting (4 bouts performed at power outputs selected to elicit fatigue in 1‐10 minutes); and (c) posttesting (4 bouts performed at the same power outputs as pretesting but completed after ingesting the supplements 4 times per day for 6 consecutive days). The results indicated that CM and CM-CHO supplementation significantly (p # 0.05) increased AWC by 9.4 and 30.7%, respectively. These data suggest that 33 g of CHO may augment the effects of CM supplementation on AWC.

Validity of percent body fat estimations in males

The present investigation examined the validity of selected skinfold (Sum3), near-infrared interactance (F5000 and F1000), and bioelectrical impedance (BIA) equations for estimating percent body fat (% fat) in young adult males (mean age +/- SD = 22 +/- 3 yr) by comparing the estimates with values obtained from underwater weighing. Fifty-seven Caucasian male (mean % fat +/- SD = 15.1 +/- 6.2) volunteers served as subjects. The statistical analyses included examination of constant error (CE), standard error of estimate (SEE), r, and total error (TE). The results indicated that the Sum3 equation had the lowest SEE (2.7% fat) and TE (3.6% fat) as well as the highest validity coefficient (r = 0.90). The F5000, however, had the lowest CE (-1.0% fat). Based on these findings, the Sum3 equation is recommended over the F5000 (TE = 4.2% fat), F1000 (TE = 6.1% fat), and BIA (TE = 5.0% fat) equations for estimating % fat in young adult males.

An examination of the electromyographic fatigue threshold test

SummaryThe purpose of this investigation was to examine times to exhaustion at various percentages of the electromyographic fatigue threshold (EMGFT). Eight adult males [mean (SD), 21 (1) years] volunteered for the investigation. EMGFT was derived by determining the rate of rise in the electrical activity of the vastus lateralis [using integrated electromyography (iEMG)] over time (iEMG slope) for four fatiguing power outputs during cycle ergometry. The four power outputs were then plotted as a function of the four iEMG slope coefficients. The y-intercept of the power output versus iEMG slope coefficient graph was defined as the EMGFT. The intraclass correlation for repeated EMGFT tests was R=0.65 (SEE=7 W) and there was no significant (P>0.05) difference between the mean (SD) values for test [260 (11) W] versus retest [262 (32) W]. Actual times to exhaustion were determined for work bouts at power outputs equal to 85, 100, 115, 130, and 145% of EMGFT. The mean (SD) times to exhaustion for these work bouts were 495 (231), 225 (72), 135 (35), 94 (17), and 72 (14) s, respectively. A power curve was derived using the mean power outputs and mean times to exhaustion from the five rides at various percentages of EMGFT. The power curve provided estimates of the power outputs which could be maintained for 30 and 60 min. There were significant (P<0.05) differences between the mean EMGFT (260 W) and the power outputs which could be maintained for 30 (151 W) and 60 (125 W) min. EMGFT overpredicted the estimated power outputs that could be maintained for 30 and 60 min by 42% and 52%, respectively.

Effects of resistance exercise and creatine supplementation on myasthenia gravis: a case study

PURPOSE The purpose of this case study was to determine the effects of 15 wk of resistance exercise and creatine (Cr) supplementation on body composition, training volume, peak strength, and complete blood chemistry in a patient with myasthenia gravis (MG). METHODS The patient was a 26-yr-old man who was taking prednisone and azathioprine for his condition. The patient self-administered 5 g of Cr per day in addition to resistance exercise 3 times per week. Fasting blood samples were obtained and body weight (BW) and fat free mass (FFM; via hydrostatic weighing) were measured before and after training and Cr supplementation. In addition, isokinetic (Cybex II) peak strength for leg extension (LE), leg flexion (LF), and volume load (repetition x mass lifted) for the first and last resistance training session were determined. RESULTS After Cr supplementation and training, the results demonstrated increases in BW (6.8%), FFM (4.3%), upper body volume load (37.0%), lower body volume load (15.0%), and peak strength for LE (37.0%) and LF (12.5%). Moreover, blood chemistry values remained within normal limits for the duration of the 15-wk study. CONCLUSION These data suggest that resistance exercise plus Cr supplementation may promote gains in strength and FFM in patients with MG.

Validity of bioelectrical impedance equations for estimating percent fat in males

The present study examined the validity of selected bioelectrical impedance (BIA) equations for estimating percent fat (% fat) in males and compared their validity with that of a commonly used skinfold equation (Sum3). One-hundred twenty-two Caucasian males (X +/- SD = 12.5 +/- 5.8% fat, as determined by underwater weighing) served as subjects. Selection of the BIA equations was dependent upon meeting at least one of three criteria: 1) developed from a previous interlaboratory investigation, 2) derived on a large sample size (> 200), or 3) previously been shown to accurately estimate body composition when cross-validated against a criterion method. Cross-validation analyses included examination of the constant error, standard error of estimate (SEE), r, and total error (TE). The Sum3 equation which resulted in the lowest SEE and TE values (2.6% fat and 3.4% fat, respectively) and the highest validity coefficient (r = 0.90, P < 0.001), most accurately estimated % fat and, therefore, was recommended over BIA equations for estimating body composition in Caucasian males with lean to average body fatness. The fat-specific interlaboratory BIA equation of Segal et al. for males < or = 20% fat (N = 107) which resulted in a TE value of 3.6% fat and the generalized equation of Guo et al. (TE = 4.1% fat) may, however, be considered as acceptable alternatives.