Joana Alves

Coexistence of carnivores in a heterogeneous landscape: habitat selection and ecological niches

Understanding distributional patterns and mechanisms used by species for habitat selection is crucial to adopt effective land management policies in terms of biodiversity conservation. A heterogeneous landscape may allow coexistence of species. That coexistence will be dependent on the availability of the resources in the habitat that has to be sufficient to fulfil their basic needs. The present study aimed to investigate habitat selection, niche breadth and niche overlap of three sympatric carnivore species (Vulpes vulpes,Genetta genetta and Martes foina) in a typically fragmented landscape from Central Portugal, using camera-trapping techniques. The results obtained revealed that the investigated species use the available habitats differently and in a non-random way. The red fox showed the most specialized behaviour, positively selecting coniferous forests. The common genet preferred eucalyptus, avoiding old-growth mixed woodland, in contrast with stone marten that exhibited a strong preference for this late habitat, avoiding eucalyptus. Concerning the niche breadth, the genet had the highest value while the red fox had the lowest one. The results obtained at the camera-trap level showed that the highest niche overlap occurred between the genet and the stone marten which suggests that these species can coexist and share the available resources. Regarding the habitat level, the greatest niche overlap was found for the stone marten and the red fox, indicating the exploration of the same general habitat conditions by both species. The results obtained in the present study support the concept that landscape complexity allows coexistence between species within the same trophic level.

The importance of lizards and small mammals as reservoirs for Borrelia lusitaniae in Portugal

Borrelia lusitaniae is a pathogen frequent in the Mediterranean area. Apart from lizards, evidence for birds and small mammals as competent reservoirs for this genospecies has been occasional. We collected questing ticks, skin biopsies and Ixodes sp. ticks feeding on lizards, birds and small mammals in a B. burgdorferi s.l. (sensu lato) enzootic area to assess their importance in the maintenance of B. lusitaniae. Borrelia lusitaniae was the most prevalent genospecies in questing ticks and was commonly found in larvae feeding on Psammodromus algirus. One biopsy infected with B. lusitaniae was collected from the tail of one Podarcis hispanica, which suggests systemic infection. Ixodes ricinus larvae feeding on Apodemus sylvaticus were infected with B. lusitaniae but with a lower prevalence. Our results reinforce the importance of lizards as reservoirs for B. lusitaniae, suggesting that P. algirus, in particular, acts as main reservoir for B. lusitaniae in Portugal.

Variation in the Abundance and Reproductive Characteristics of Great Tits Parus major in Forest and Monoculture Plantations

Abstract. Natural forests have been progressively replaced by other forest types, mainly monoculture plantations. We compared the abundance of Great Tits and its main food competitors, the Chaffinch Fringilla coelebs and other tits species (Blue Tit Cyanistes caeruleus, Coal Tit Periparus ater and Crested Tit Lophophanes cristatus), and breeding parameters of Great Tits in four forest types: a mixed forest, a native broadleaf forest, a Maritime Pine Pinus pinaster plantation and a Blue Gum Eucalyptus globulus plantation in Central Portugal. The breeding parameters (laying date, clutch size, egg volume, hatching success, fledging success and chick body mass) were collected from nest boxes placed in the four forest types. The censuses indicated a higher abundance index of Great Tit and its food competitors in the mixed and broadleaf forest, when compared to the monoculture plantations. Occupancy rate of nest boxes was not directly related with tit abundance due to differences in the availability of natural nest cavities among forest types, and differences in preference for nesting cavities among tit species. However, a Great Tit abundance index gave similar results to the densities described in the literature, except for the Great Tit in the Eucalyptus plantations where the placement of the nest boxes should explain our value of 2.1 birds ha-1, which is four times higher than that reported for other Eucalyptus plantations. Egg volume differed significantly among forest types; it was higher in the pine plantations and lower in the Eucalyptus plantations. Surprisingly, all other breeding parameters (laying date, clutch size, chick body mass, hatching and fledgling success) were similar among forest types and between years. On the other hand the number of eggs, hatchlings and fledglings were lower than those obtained in other studies in the Iberian Peninsula. Our results suggest that Great Tits have a high plasticity that enables them to adjust their breeding strategies even to forest plantations, if nest sites are available.

The effect of male age on patterns of sexual segregation in Siberian ibex

Sexual segregation is very common in sexually size dimorphic ungulates and may be the result of different habitat preferences and/or differential social behaviours of males and females. Various hypotheses have been put forward to explain this phenomenon. In the present research, we examined sexual segregation in a quite poorly understood species, the Siberian ibex. The species presents a marked sexual size dimorphism, with adult males weighing double as much as females. We use the Sexual Segregation and Aggregation Statistics (SSAS) to analyze the sex-age patterns of sexual segregation in this species, to understand the relevance of social factors. Our results show that adult Siberian ibex males were socially segregated from females all year round, except during the rutting season. Furthermore, the degree of segregation between females and males was influenced by the age of males. Moreover, the patterns of social segregation within males also increased with male age, reaching maximum values for males of 9 years-old and older, which means male age plays an important role in the sexual segregation of this species. This study clearly shows that social factors play a key role in the sexual segregation of Siberian ibex.

Repeated Mesenchymal Stromal Cell Treatment Sustainably Alleviates Machado-Joseph Disease

Machado-Joseph disease (MJD) or spinocerebellar ataxia type 3, the most common dominant spinocerebellar ataxia (SCA) worldwide, is caused by over-repetition of a CAG repeat in the ATXN3/MJD1 gene, which translates into a polyglutamine tract within the ataxin-3 protein. There is no treatment for this fatal disorder. Despite evidence of the safety and efficacy of mesenchymal stromal cells (MSCs) in delaying SCA disease progression in exploratory clinical trials, unanticipated regression of patients to the status prior to treatment makes the investigation of causes and solutions urgent and imperative. In the present study, we compared the efficacy of a single intracranial injection with repeated systemic MSC administration in alleviating the MJD phenotype of two strongly severe genetic rodent models. We found that a single MSC transplantation only produces transient effects, whereas periodic administration promotes sustained motor behavior and neuropathology alleviation, suggesting that MSC therapies should be re-designed to get sustained beneficial results in clinical practice. Furthermore, MSC promoted neuroprotection, increased the levels of GABA and glutamate, and decreased the levels of Myo-inositol, which correlated with motor improvements, indicating that these metabolites may serve as valid neurospectroscopic biomarkers of disease and treatment. This study makes important contributions to the design of new clinical approaches for MJD and other SCAs/polyglutamine disorders.