João Bedendo

Torque teno virus among dialysis and renal-transplant patients

Patients who undergo dialysis treatment or a renal transplant have a high risk of blood-borne viral infections, including the Torque teno virus (TTV). This study identified the presence of TTV and its genome groups in blood samples from 118 patients in dialysis and 50 renal-transplant recipients. The research was conducted in a hospital in the city of Maringá, state of Paraná. The viral DNA, obtained from whole blood, was identified by using two nested Polymerase Chain Reactions (PCR). The frequencies of TTV were 17% and 36% in dialysis patients using the methodology proposed by Nishizawa et al . (1997) and Devalle and Niel (2004) , respectively, and 10% and 54% among renal-transplant patients. There was no statistically significant association between the frequency of the pathogen and the variables: gender, time in dialysis, time since transplant, blood transfusions, and the concomitant presence of hepatitis B, for either the dialysis patients or the renal-transplant recipients. Among dialysis patients and renal-transplant recipients, genogroup 5 was predominant (48% and 66% respectively), followed by genogroup 4 (37% and 48%) and genogroup 1 (23% and 25%). Genogroup 2 was present in both groups of patients. Some patients had several genogroups, but 46% of the dialysis patients and 51% of the renal-transplant recipients had only a single genogroup. This study showed a high prevalence of TTV in dialysis patients and renal-transplant recipients.

Phenotypic and genotypic profile of Staphylococcus aureus isolated in nursing students, 2008

Objective: To investigate the prevalence of nasal entrainment, phenotypic and genotypic profile of Staphylococcus aureus, as isolated from nursing students. Methods: A cross-sectional population of 101 students enrolled in the first three grades of the undergraduate nursing course in 2008. Staphylococcus aureus was isolated from biological material obtained from the swab through the nasal vestibules. Susceptibility to oxacillin and vancomycin was determined by minimum inhibitory concentration test. The mecA gene was identified by testing the polymerase chain reaction. Results: There was a 90.1% positive finding of Staphylococcus aureus. The frequency of oxacillin resistance was 9.8%; all samples were sensitive to vancomycin. The eight strains resistant to oxacillin carried the mecA gene. Conclusion: The prevalence of Staphylococcus aureus was high. Oxacillin resistance was significant, but all strains were sensitive to vancomycin. Isolates resistant to

Perfil fenotípico e genotípico de Staphylococcus aureus isolados de estudantes de enfermagem, 2008

Objective: To investigate the prevalence of nasal entrainment, phenotypic and genotypic profile of Staphylococcus aureus, as isolated from nursing students. Methods: A cross-sectional population of 101 students enrolled in the first three grades of the undergraduate nursing course in 2008. Staphylococcus aureus was isolated from biological material obtained from the swab through the nasal vestibules. Susceptibility to oxacillin and vancomycin was determined by minimum inhibitory concentration test. The mecA gene was identified by testing the polymerase chain reaction. Results: There was a 90.1% positive finding of Staphylococcus aureus. The frequency of oxacillin resistance was 9.8%; all samples were sensitive to vancomycin. The eight strains resistant to oxacillin carried the mecA gene. Conclusion: The prevalence of Staphylococcus aureus was high. Oxacillin resistance was significant, but all strains were sensitive to vancomycin. Isolates resistant to

Perfil epidemiológico dos óbitos em terapia renal substitutiva e custo do tratamento

Abstract Objective: Determine the epidemiological profile of patient deaths related to renal replacement therapy and the cost of treatment.Methods: Cross-sectional retrospective study carried out with data from the National Renal Replacement Therapy Database. It included 2009 deaths due to renal failure. Data were analyzed using statistical inference and nonparametric statistics.Results: A greater number of deaths was observed among white, male patients. The cost of treatment increased 6.7% in the state and 45.3% in the city.Conclusion: The highest number of deaths occurred among white males; there were marked variations in hospital mortality rates; and the cost of treatment increased over the past three years. Resumo Objetivo: Descrever o perfil epidemiologico dos obitos de pacientes em terapia renal substitutiva e o custo do tratamento.Metodos: Estudo transversal e retrospectivo realizado com os dados da Base Nacional de Terapia Renal Substitutiva. Foram incluidos 2029 obitos por insuficiencia renal. Os dados foram analisados por inferencia estatistica e estatistica nao parametrica.Resultados: Houve maior numero de obitos entre os pacientes do sexo masculino e da raca branca. O custo do tratamento aumentou 6,7% nos gastos do estado e 45,3% no municipio. Conclusao: O maior numero de obitos ocorreu no sexo masculino, raca branca, variacoes bruscas nas taxas de mortalidade hospitalar e aumentou o custo do tratamento nos ultimos tres anos.

RISK FACTORS ASSOCIATED WITH TEMPORARY CATHETER-RELATED INFECTION IN PATIENTS ON DIALYSIS TREATMENT

Este estudio tuvo como objetivo identificar los factores de riesgo asociados a la infeccion de cateter provisorio para hemodialisis en pacientes en tratamiento dialitico en un hospital escuela. Se trata de un estudio prospectivo, realizado en el periodo entre noviembre de 2012 y mayo de 2013, con 129 pacientes. Fueron colectados: registros sociodemograficos, clinicos y bioquimicos de los participantes se obtuvieron de los registros medicos. Entre los pacientes que fueron sometidos a la implantacion del cateter, el 48,8% presentaron infeccion relacionada con cateter, el 65% eran del sexo masculino, el 65% tenian edad igual a 60 anos o mas, el 85,3% utilizaron el cateter porque hubieran sido diagnosticados con insuficiencia renal aguda y el 88% fueron hospitalizados en la unidad de terapia intensiva. Hubo correlacion significativa entre las variables independientes y el resultado infeccion. Los factores de riesgo asociados a la infeccion relacionada a la implantacion del cateter temporal para hemodialisis fueron: tiempo de permanencia del cateter, la troca del dispositivo, obito y complicaciones durante la sesion de hemodialisis.

FACTORES DE RIESGO ASOCIADOS A LA INFECCIÓN DE CATÉTER PROVISORIO EN PACIENTES EN TRATAMIENTO DIALÍTICO

Este estudio tuvo como objetivo identificar los factores de riesgo asociados a la infeccion de cateter provisorio para hemodialisis en pacientes en tratamiento dialitico en un hospital escuela. Se trata de un estudio prospectivo, realizado en el periodo entre noviembre de 2012 y mayo de 2013, con 129 pacientes. Fueron colectados: registros sociodemograficos, clinicos y bioquimicos de los participantes se obtuvieron de los registros medicos. Entre los pacientes que fueron sometidos a la implantacion del cateter, el 48,8% presentaron infeccion relacionada con cateter, el 65% eran del sexo masculino, el 65% tenian edad igual a 60 anos o mas, el 85,3% utilizaron el cateter porque hubieran sido diagnosticados con insuficiencia renal aguda y el 88% fueron hospitalizados en la unidad de terapia intensiva. Hubo correlacion significativa entre las variables independientes y el resultado infeccion. Los factores de riesgo asociados a la infeccion relacionada a la implantacion del cateter temporal para hemodialisis fueron: tiempo de permanencia del cateter, la troca del dispositivo, obito y complicaciones durante la sesion de hemodialisis.

FATORES DE RISCO ASSOCIADOS À INFECÇÃO DE CATETER PROVISÓRIO EM PACIENTES SOB TRATAMENTO DIALÍTICO

Este estudio tuvo como objetivo identificar los factores de riesgo asociados a la infeccion de cateter provisorio para hemodialisis en pacientes en tratamiento dialitico en un hospital escuela. Se trata de un estudio prospectivo, realizado en el periodo entre noviembre de 2012 y mayo de 2013, con 129 pacientes. Fueron colectados: registros sociodemograficos, clinicos y bioquimicos de los participantes se obtuvieron de los registros medicos. Entre los pacientes que fueron sometidos a la implantacion del cateter, el 48,8% presentaron infeccion relacionada con cateter, el 65% eran del sexo masculino, el 65% tenian edad igual a 60 anos o mas, el 85,3% utilizaron el cateter porque hubieran sido diagnosticados con insuficiencia renal aguda y el 88% fueron hospitalizados en la unidad de terapia intensiva. Hubo correlacion significativa entre las variables independientes y el resultado infeccion. Los factores de riesgo asociados a la infeccion relacionada a la implantacion del cateter temporal para hemodialisis fueron: tiempo de permanencia del cateter, la troca del dispositivo, obito y complicaciones durante la sesion de hemodialisis.

Perfil fenotípico y genotípico del Staphylococcus aureus aislados de estudiantes de enfermería, 2008

Objective: To investigate the prevalence of nasal entrainment, phenotypic and genotypic profile of Staphylococcus aureus, as isolated from nursing students. Methods: A cross-sectional population of 101 students enrolled in the first three grades of the undergraduate nursing course in 2008. Staphylococcus aureus was isolated from biological material obtained from the swab through the nasal vestibules. Susceptibility to oxacillin and vancomycin was determined by minimum inhibitory concentration test. The mecA gene was identified by testing the polymerase chain reaction. Results: There was a 90.1% positive finding of Staphylococcus aureus. The frequency of oxacillin resistance was 9.8%; all samples were sensitive to vancomycin. The eight strains resistant to oxacillin carried the mecA gene. Conclusion: The prevalence of Staphylococcus aureus was high. Oxacillin resistance was significant, but all strains were sensitive to vancomycin. Isolates resistant to