João J. Ferreira

The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci

Chromatin states have to be faithfully duplicated during DNA replication to maintain cell identity. It is unclear whether or how ATP-dependent chromatin-remodelling factors are involved in this process. Here we provide evidence that the Williams syndrome transcription factor (WSTF) is targeted to replication foci through direct interaction with the DNA clamp PCNA, an important coordinator of DNA and chromatin replication. WSTF, in turn, recruits imitation switch (ISWI)-type nucleosome-remodelling factor SNF2H to replication sites. These findings reveal a novel recruitment mechanism for ATP-dependent chromatin-remodelling factors that is fundamentally different from the previously documented targeting by sequence-specific transcriptional regulators. RNA-interference-mediated depletion of WSTF or SNF2H causes a compaction of newly replicated chromatin and increases the amount of heterochromatin markers, including HP1β. This increase in the amount of HP1β protein is mediated by progression through S phase and is not the result of an increase in HP1β mRNA levels. We propose that the WSTF–ISWI complex has a role in the maintenance of chromatin structures during DNA replication.

A model of entrepreneurial intention

Purpose – The field of entrepreneurship is dotted across different paradigms. Measuring entrepreneurial intention automatically needs to incorporate insights from the psychological approach and behavioural approach. The purpose of this study was to develop and test a comprehensive structural equation model which combines both psychological and behavioural perspectives aiming to identify what variables have influence on entrepreneurial intention of secondary students.Design/methodology/approach – This research was developed involving a sample of secondary students. The method of data collection was a survey by self‐administered questionnaire, to two secondary student classes, with several groups of questions related to demographic characteristics, behavioural and psychological constructs and entrepreneurial intention. Data was analysed using structural equation modelling (SEM).Findings – The results show that need for achievement, self‐confidence, and personal attitude positively affect entrepreneurial int...

SME Innovative Capacity, Competitive Advantage and Performance in a ‘Traditional’ Industrial Region of Portugal

This study aims to identify the factors that contribute to the building of a firm’s innovative capacity and to assess the way in which this contributes to our understanding of how much this contributes to improvements in the firm’s performance. A conceptual model is proposed consisting of five separate dimensions: the firm; the entrepreneur; the external business environment; the firm’s innovative capacity; and the firm’s performance. The study was based on questionnaire-based data from a sample of firms drawn from the manufacturing industry in the Beira Interior Region of Portugal. The results provided evidence regarding the factors influencing the innovative capacity of firms and permitted conclusions to be drawn regarding effects of superior innovative capacity on the construction of firms’ competitive advantage, which in turn contributes to improved performance.

Entrepreneurship Education: How Psychological, Demographic and Behavioural Factors Predict the Entrepreneurial Intention.

Purpose – Based on the Theory of Planned Behaviour (TPB), the purpose of this paper is to approach entrepreneurial intention (EI) and the factors preceding the founding of EI among secondary students, both studying general academic and specific professional programs, and thereby establish causal relationships between psychological, demographic and behavioural factors. Design/methodology/approach – A sample of secondary students, both studying general academic and specific professional programs, was chosen. Data were collected through questionnaires and analysed with resource to structural equation models (SEM) to measure the causal relationships among different constructs. Findings – The results show that the EI of secondary school students is influenced by such demographic, psychological and behavioural factors. However, the hypothesis of becoming entrepreneurs in the future remains independent of the fact of whether or not students had already experienced engagement with entrepreneurship‐related themes having no impact on EI. These results raise a series of pertinent questions and deserve being subject to a more profound reflection and not only among the scientific and academic community but also among political and government entities. Originality/value – The paper provides important evidence to expand entrepreneurship education specifically in the field of secondary school education. These students are already about to face choices over their professional careers. This remains valid whether or not students intend to proceed with higher education studies or to join the workforce. Therefore this research contributes towards meeting a better understanding of EI and the factors preceding the formation of this intention among secondary school students.

Entrepreneurship education and the propensity for business creation: testing a structural model

This study aims to identify the factors that contribute the most to the intention to start up a business. The research also aims to identify the profile of student who is a potential entrepreneur concerning several aspects: personal attributes, family, demographic variables and motivations. Based on a sample of university students, a structural model was tested. Research findings include the idea that entrepreneurship education is the most relevant factor in the propensity for business creation. On the other hand, personal characteristics have an important role in shaping the motivation to start up a business and perceived hurdles have a negative impact on the intention to start one up.

Knowledge Spillovers: Cooperation between Universities and KIBS

Knowledge is increasingly perceived as a fulcral factor to company competitiveness. As the transfer of knowledge is one of the key functions of knowledge intensive business services (KIBS), this research seeks to analyse how the transfer of knowledge takes place between the higher education sector and KIBS type firms. The empirical results show that cooperation between KIBS and universities occurs irrespective of the typology (whether professional or technological in focus). We also empirically establish how geographic proximity is the factor bearing greatest influence over cooperation while such cooperation proves to have a positive impact on the company capacity to innovate. We furthermore find that this innovation capacity also drives a positive company financial performance.

Integrating Metacognitive and Psychometric Decision-Making Approaches for Bank Customer Loyalty Measurement

Greater competition and the aftermath of the financial crisis have meant that banks are increasingly focused on improving service quality and achieving higher levels of customer loyalty. Doing so requires being able to identify, understand and measure the determinants of such loyalty, however; and given the variety of multi-faceted factors involved, and the interrelationships among them, this is a complex undertaking. This paper proposes and tests an integrated application of cognitive mapping and multiple criteria decision analysis (MCDA), thus combining metacognitive and psychometric decision-making approaches, to create a framework for the assessment of bank customer loyalty, which identifies its determinants and allows the trade-offs among them to be calculated. Practical advantages and limitations of our proposal are also discussed.

Assessing credit risk of mortgage lending using MACBETH: a methodological framework

– Considered the largest investment for most households, buying a house requires careful and transparent analysis by all parties involved in the transaction. The aim of this paper is to propose a methodological framework allowing for the readjustment of trade-offs among risk evaluation criteria, considered of extreme importance in the lending decision process of mortgage loans. , – Multiple criteria decision analysis (MCDA) has proved over the years to be effective and versatile in handling compensations among criteria. Measuring attractiveness is applied by a categorical based evaluation technique (MACBETH) to a pre-established structure of credit-scoring criteria for mortgage lending risk evaluation. This pre-established structure is currently used by one of the largest banks in Portugal. , – The framework allowed the authors to provide the credit experts who participated in the study with a more informed, transparent and accurate mortgage-lending risk-evaluation system. The sensitivity and robustness analyses carried out also helped in promoting discussion and supporting the readjustments made. , – The study shows the usefulness of using the MACBETH approach to assist credit analysts in making better informed decisions, and opens avenues for further research. However, due to the dependence on the participants involved, extrapolations without proper caution are discouraged. , – The credit analysts who participated in this study considered the framework more discerning in terms of Basel directives. , – The integration of MACBETH and credit-scoring mechanisms holds great potential for risk assessment and decision support. No prior work reporting the application of MACBETH in terms of mortgage-lending risk-evaluation is known.

Radio frequency identification: a case study of healthcare organisations

RFID technology is actually considered a hot topic in all scientific areas and has been described as a major enabling technology for the automation of many processes. Although it is not a new technology it has only recently come to the awareness of the public and is widely used in many sectors and particularly in healthcare. This paper aims to illustrate the utilisation of the RFID technology in healthcare, more precisely in infant security systems within the hospitals. To achieve this objective a case study about the experience of Portuguese hospitals in this system is presented and highlighted the main advantages reached with its utilisation.

A comparative benchmarking analysis of main Iberian container terminals: a DEA approach

Benchmarking is an important tool to organisations to improve their productivity, product quality, process efficiency or services. From Benchmarking the organisations could compare their performance with competitors and identify their strengths and weaknesses. This study intends to do a benchmarking analysis on the main Iberian Sea ports with a special focus on their container terminals efficiency. To attain this, the DEA (data envelopment analysis) is used since it is considered by several researchers as the most effective method to quantify a set of key performance indicators. In order to reach a more reliable diagnosis tool the DEA is used together with the data mining in comparing the sea ports operational data of container terminals during 2007. Taking into account that sea ports are global logistics networks the performance evaluation is essential to an effective decision making in order to improve their efficiency and, therefore, their competitiveness.