João Soares

Angiotensin II acutely decreases myocardial stiffness: a novel AT1, PKC and Na+/H+ exchanger-mediated effect.

Acute effects of angiotensin II (AngII) on diastolic properties of the myocardium were investigated. Increasing concentrations of AngII (10−9 to 10−5 M) were added to rabbit papillary muscles in the absence (n=11) or presence of: (i) AT1 receptor antagonists, losartan (10−6 M; n=7) or ZD‐7155 (10−7 M; n=8); (ii) ZD‐7155 (10−7 M) plus AT2 receptor antagonist PD‐123,319 (2 × 10−6 M; n=6); (iii) PKC inhibitor, chelerythrine (10−5 M; n=8); or (iv) Na+/H+ exchanger (NHE) inhibitor, 5‐(N‐methyl‐N‐isobutyl)‐amiloride (10−6 M; n=10). Passive length–tension relations were constructed before and after a single concentration of AngII (10−5 M, n=6). Effects of AngII infusion (10 μg kg−1 min−1) were evaluated in in situ rabbit hearts. AngII concentration dependently increased inotropy and resting muscle length (RL). At 10−5 M, active tension increased 43.3±6.25% and RL 1.96±0.4%. Correcting RL to its initial value resulted in a 46±4% decrease of resting tension, indicating decreased muscle stiffness, as confirmed by the right and downward shift of the passive length–tension relation promoted by AngII. In the intact heart, at matched systolic pressures of 112 mmHg, AngII decreased end‐diastolic pressures from 10.3±0.3 to 5.9±0.5 mmHg, and minimal diastolic pressures from 8.4±0.5 to 4.6±0.6 mmHg. AT1 blockade inhibited AngII effects on myocardial inotropy and stiffness, while PKC or NHE inhibition only significantly attenuated its effects on resting length and tension. In conclusion, AngII decreases myocardial stiffness, an effect that requires AT1 receptor activation and is mediated by PKC and NHE. This represents a novel mechanism of acute neurohumoral modulation of diastolic function, suggesting that AngII is a powerful regulator of cardiac filling.

Development and performance of an advanced ejector cooling system for a sustainable built environment

Ejector refrigeration is a promising technology for the integration into solar driven cooling systems because of its relative simplicity and low initial cost. The major drawback of such a system is associated to its relatively low coefficient of performance (COP) under variable operating conditions. In order to overcome this problem, an advanced ejector was developed that changes its geometrical features depending on the upstream and downstream conditions. This paper provides a short overview of the development process and results of a small cooling capacity (1.5 kW) solar driven cooling system using a variable geometry ejector. During the design steps, a number of theoretical works have been carried out, including the selection of the working fluid, the determination of the geometrical requirements and prototype design. Based on the analysis, R600a was selected as working fluid. A prototype was constructed with two independent variable geometrical factors: the area ratio and the nozzle exit position. A test rig was also assembled in order to test the ejector performance under controlled laboratory conditions and to elaborate a control algorithm for the variable geometry. Ejector performance was assessed by calculation of cooling cycle COP, entrainment ratio and critical back pressure. The results show that for a condenser pressure of 3 bar, an 80% increase in the COP was obtained when compared to the performance of a fixed geometry ejector. Experimental COP values varied between 0.4 and 0.8, depending on operating conditions. Currently the cooling cycle is being integrated into a solar driven demonstration site for long term “in situ” assessment.

Numerical simulation of a hybrid CSP/Biomass 5 MWel power plant

The fundamental benefit of using renewable energy systems is undeniable since they rely on a source that will not run out. Nevertheless, they strongly depend on meteorological conditions (solar, wind, etc.), leading to uncertainty of instantaneous energy supply and consequently to grid connection issues. An interesting concept is renewable hybridisation. This consists in the strategic combination of different renewable sources in the power generation portfolio by taking advantage of each technology. Hybridisation of concentrating solar power with biomass denotes a powerful way of assuring system stability and reliability. The main advantage is dispatchability through the whole extent of the operating range. Regarding concentrating solar power heat transfer fluid, direct steam generation is one of the most interesting concepts. Nevertheless, it presents itself technical challenges that are mostly related to the two-phase fluid flow in horizontal pipes, as well as the design of an energy storage system. Als...