Joe Sakurai
St. Marianna University School of Medicine
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Joe Sakurai.
Surgery Today | 2006
Masafumi Katayama; Hiroshi Nakano; Atsuko Ishiuchi; Wenwen Wu; Ryuichi Oshima; Joe Sakurai; Hiroyuki Nishikawa; Susumu Yamaguchi; Takehito Otsubo
PurposeThe pivotal metastatic processes of colorectal cancer (CRC) have yet to be fully investigated by a comprehensive all-inclusive protein analysis. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) and liquid chromatography–tandem mass spectrometry (LC/MS/MS) to investigate the protein pattern changes during the metastasis of CRC. Two CRC cell lines were investigated: SW480 derived from the primary lesion and SW620 derived from lymph node metastasis in the same patient.MethodsThe two cell lines were compared using 2D-DIGE with a maleimide CyDye fluorescent protein labeling technique, which has an enhanced sensitivity for many proteins at a low concentration. A comprehensive proteomics analysis was performed by the dual-labeling method using Cy3 and Cy5 and by LC/MS/MS. In addition, an in vivo experiment of metastasis using nude mice was performed by the injection of the two cell lines into the spleen.ResultsAmong approximately 1 500 proteins, we detected 9 protein spots with definitively significant changes between the two cell lines. Three out of the nine proteins were validated by a Western blot analysis. Alpha-enolase and triosephosphate isomerase were significantly upregulated in SW620 in comparison to SW480. Annexin A2 (annexin II) was significantly downregulated in SW620 compared to SW480. Neither liver metastasis nor peritoneal dissemination was established in the metastatic experiment using SW480 but some liver and peritoneal metastases occurred in the experiment using SW620. An in vivo metastatic experiment using SW620 showed the expressions of alpha-enolase and triosephosphate isomerase to increase in the liver metastases in comparison to those in the splenic implanted lesion. The expressions of triosephosphate isomerase increased in the peritoneal lesions in comparison to those in the splenic implanted lesion.Conclusions2D-DIGE and LC/MS/MS techniques identified nine proteins that increased significantly more in SW620 than in SW480. The finding of our in vivo metastatic experiment suggests that alpha-enolase and triosephosphate isomerase, at least in part, may be associated with the metastatic process of these two cell lines.
Surgery Today | 2010
Shinjiro Kobayashi; Takeshi Asakura; Nobuyuki Ohike; Takeharu Enomoto; Joe Sakurai; Satoshi Koizumi; Taiji Watanabe; Hiroshi Nakano; Takehito Otsubo
The patient was a 75-year-old asymptomatic man, in whom a tumor mass in the pancreatic tail had been found 6 months earlier. Computed tomography revealed a mass 7 cm in diameter, and an enhancement with contrast medium was observed at the periphery and partially inside the mass, but not in most parts of the tumor. Endoscopic retrograde cholangiopancreatography showed a filling defect in the main pancreatic duct. A distal pancreatectomy was performed because of the possibility of a malignant tumor. The tumor consisted of a lobular invasive growth component and a component with intraductal growth into the main pancreatic duct, and histologically the tumor cells had solid acinar to partially trabecular/tubular patterns. Trypsin (an acinic cell marker) expression was widely observed, followed by the expression of chromogranin A (an endocrine cell marker) in about 30% of the tumor cells. The tumor was diagnosed as mixed acinar-endocrine carcinoma according to the WHO classification.
International Journal of Clinical Oncology | 2011
Kazuhiro Miura; Hiroshi Nakano; Joe Sakurai; Shinjiro Kobayashi; Satoshi Koizumi; Tatsuhiro Arai; Tsukasa Shimamura; Ryoji Makizumi; Kyoji Yamada; Nobuyoshi Miyajima; Takehito Otsubo; Junki Koike
World Journal of Surgery | 2014
Shinjiro Kobayashi; Ryuiti Ooshima; Satoshi Koizumi; Masafumi Katayama; Joe Sakurai; Taiji Watanabe; Hiroshi Nakano; Toshihide Imaizumi; Takehito Otsubo
Hepato-gastroenterology | 2008
Hiroshi Nakano; Takeshi Asakura; Joe Sakurai; Satoshi Koizumi; Takayuki Asano; Taiji Watanabe; Takehito Otsubo
Nihon Rinsho Geka Gakkai Zasshi (journal of Japan Surgical Association) | 2008
Natsuko Kamei; Joe Sakurai; Masafumi Katayama; Tadashi Suda; Satoshi Tsukikawa; Takehito Ohtsubo
Nihon Rinsho Geka Gakkai Zasshi (journal of Japan Surgical Association) | 2009
Tsukasa Shimamura; Shinjirou Kobayashi; Yuji Jinnouchi; Joe Sakurai; Naotaka Tobe; Ryoji Makizumi; Tadashi Suda; Satoshi Tsukikawa; Nobuyoshi Miyajima; Takehito Otsubo
Nihon Rinsho Geka Gakkai Zasshi (journal of Japan Surgical Association) | 2008
Ryuichi Oshima; Eiji Minato; Joe Sakurai; Kazuhiko Yoshida; Hisashi Shimada; Takehito Otsubo
Jpn J Gastroenterol Surg, Nihon Shokaki Geka Gakkai zasshi | 2008
Joe Sakurai; Takeharu Enomoto; Kouhei Segami; Akiyoshi Noda; Masafumi Katayama; Toshiyuki Suwa; Shinjirou Kobayashi; Hiroshi Nakano; Ichiro Tanaka; Takehito Otsubo
Nihon Rinsho Geka Gakkai Zasshi (journal of Japan Surgical Association) | 2000
Joe Sakurai; Kazuhiko Yoshida; Kyoji Yamada; Hisashi Shimada; Susumu Yamaguchi