Johannes Reisch

Secondary metabolites produced by callus cultures of various Ruta species

Callus cultures were established from hypocotyl explants of R. bracteosa, R. chalepensis and R. macrophylla. Calli were maintained for more than three years on MS-medium supplemented with 1 mg l-1 of each 2,4-D and kinetin. Acridone and furoquinoline alkaloids and coumarins have been isolated from four week old calli grown on a hormone containing and hormone-free medium. A new chlorinated acridone alkaloid has been detected.

Natural Product Chemistry, 147. Synthesis of some coumarin derivatives

Two naturally occurring coumarins, dihydrosuberenol (5) and (E)-O-ethylsuberenol (6), and another possible natural product (E)-O-methylsuberenol (7), were synthesized from (E)-suberenol (1). The treatment of 1 with HBr gave the new menthadienediylbis(coumarin) 8.