John A. Gatehouse
University of Newcastle
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Featured researches published by John A. Gatehouse.
Molecular Breeding | 2001
Shahina Bano Maqbool; Sheikh Riazuddin; Nguyen Thi Loc; Angharad M. R. Gatehouse; John A. Gatehouse; Paul Christou
We report the simultaneous introduction of three insecticidal genes (the Bt genes cry1Ac and cry2A, and the snowdrop lectin gene gna) into commercially important indica rice varieties M7 and Basmati 370, by particle bombardment. Transgenic plants expressed Cry1Ac, Cry2A and GNA at different levels, either singly or in combination at 0.03–1%, 0.01–0.5% and 0.01–2.5% of total soluble protein, respectively. The transgenes showed stable transmission and expression, and R1 transgenic plants provided significant (p<0.01) protection against three of the most important insect pests of rice: rice leaf folder (Cnaphalocrocis medinalis), yellow stemborer (Scirpophaga incertulas) and brown planthopper (Nilaparvata lugens). The triple transformants showed significantly (p<0.05) higher resistance to these insects than plants expressing single transgenes. Bioassays using the triple-transgenic plants showed 100% eradication of the rice leaf folder and yellow stem borer, and 25% reduction in the survival of the brown planthopper. The greatest reduction in insect survival, and the greatest reduction in plant damage, occurred in plants expressing all three transgenes. This approach maximises the utility of gene transfer technology to introduce combinations of genes whose products disrupt different biochemical or physiological processes in the same insect, providing a multi-mechanism defence.
Archive | 1987
Vaughan A. Hilder; Angharad Margaret Roscoe Gatehouse; John A. Gatehouse; David Boulter
Archive | 1984
Donald Boulter; Angharad Margaret Roscoe Gatehouse; John A. Gatehouse; Roger Bertram Cox
Archive | 1992
Vaughan Alan Hilder; Angharad M. R. Gatehouser; John A. Gatehouse; Donald Boulter; Richard F. Barker; Michael Bevan
Archive | 1991
Vaughan Alan Hilder; Angharad Margaret Ro Gatehouse; John A. Gatehouse; Donald Boulter
Archive | 2001
Nguyen Thi Loc; Paul Christou; John A. Gatehouse
British crop Protection Conference - Biotechnology in Crop Protection: Facts and Fallacies | 1998
Angharad M. R. Gatehouse; David P. Bown; H. S. Wilkinson; Rachel E. Down; Louise Ford; John A. Gatehouse; Howard A. Bell; John P. Edwards
Archive | 2003
Nguyen Thi Loc; Paul Christou; John A. Gatehouse
The BCPC Conference: Pests and diseases, Volume 2. Proceedings of an international conference held at the Brighton Hilton Metropole Hotel, Brighton, UK, 13-16 November 2000 | 2000
Angharad M. R. Gatehouse; Rachel E. Down; John A. Gatehouse; Paul Christou
Archive | 1995
Vaughan Alan Hilder; Angharad Margaret Ro Gatehouse; John A. Gatehouse; David Boulter